β-caryophyllene, a dietary cannabinoid, complexed with β-cyclodextrin produced anti-hyperalgesic effect involving the inhibition of Fos expression in superficial dorsal horn Lucindo J. Quintans-J´unior, Adriano A.S. Ara´ujo, Renan G. Brito, Priscila L. Santos, Jullyana S.S. Quintans, Paula P. Menezes, Mairim R. Serafini, Gabriel F. Silva, Flavio M.S. Carvalho, Nicole K. Brogden, Kathleen A. Sluka PII: DOI: Reference:
S0024-3205(16)30099-6 doi: 10.1016/j.lfs.2016.02.049 LFS 14734
To appear in:
Life Sciences
Received date: Revised date: Accepted date:
25 October 2015 11 February 2016 11 February 2016
Please cite this article as: Quintans-J´ unior Lucindo J., Ara´ ujo Adriano A.S., Brito Renan G., Santos Priscila L., Quintans Jullyana S.S., Menezes Paula P., Serafini Mairim R., Silva Gabriel F., Carvalho Flavio M.S., Brogden Nicole K., Sluka Kathleen A., β-caryophyllene, a dietary cannabinoid, complexed with β-cyclodextrin produced anti-hyperalgesic effect involving the inhibition of Fos expression in superficial dorsal horn, Life Sciences (2016), doi: 10.1016/j.lfs.2016.02.049
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT β-caryophyllene, a dietary cannabinoid, complexed with β-cyclodextrin produced anti-hyperalgesic effect involving the inhibition of Fos expression in superficial
PT
dorsal horn
RI
Lucindo J. Quintans-Júnior1,3,#*, Adriano A.S. Araújo2,#, Renan G. Brito1,3, Priscila L.
SC
Santos1, Jullyana S.S. Quintans1, Paula P. Menezes2, Mairim R. Serafini2, Gabriel F.
2
Department of Physiology,
Department of Pharmacy.
3
Department of Food
MA
1
NU
Silva3, Flavio M.S. Carvalho4, Nicole K. Brogden5, Kathleen A. Sluka6
Engineering. Federal University of Sergipe, São Cristóvão, Sergipe, Brazil. Department of Mineralogy and Petrology, University of São Paulo, São Paulo, Brazil.
5
Department of Pharmaceutical Sciences and Experimental Therapeutics, College of
TE
D
4
Pharmacy, University of Iowa, Iowa City, Iowa, USA. Physical Therapy and Rehabilitation Science, Carver College of Medicine, University
AC CE P
6
of Iowa, Iowa City, Iowa, USA.
#
These authors contributed equally for the development of the paper.
*Author for correspondence: Lucindo J. Quintans-Júnior, Department of Physiology, Laboratory of Neuroscience and Pharmacological Assay. Federal University of Sergipe, Av. Marechal Rondon, s/n, São Cristóvão, Sergipe, Brazil (fax +55-79-2105-6640, Email: [email protected]; [email protected]).
Conflict of interest: The authors report no conflict of interest.
1
ACCEPTED MANUSCRIPT Abstract Aims: Evaluate the anti-hyperalgesic effect of the complex containing β-caryophyllene
PT
(βCP) and β-cyclodextrin (βCD) in a non-inflammatory chronic muscle pain mice model and investigated its action on superficial dorsal horn of the lumbar spinal cord.
RI
Main Methods: The βCP-βCD complex were prepared and characterized through the
SC
DSC, TG/DTG, FTIR, XRD and SEM. The model of chronic muscle pain was induced by two injections of pH 4.0 saline (20 µl) into left gastrocnemius 5 days apart. After
NU
confirming hyperalgesia, male mice were treated with βCP-βCD (10 or 20 mg/kg; p.o.)
MA
or vehicle (saline 0,9%, p.o.) daily for 9 days. 1 h after, the mechanical hyperalgesia, muscle withdrawal thresholds and motor performance were evaluated. To evaluate the βCP-βCD action on spinal cord, animals induced with chronic muscle pain were treated
TE
D
with βCP-βCD (20 mg/kg; p.o.) or vehicle (saline 0,9%, p.o.) and 90 min. after, were perfused, the lumbar spinal cord collected, crioprotected, cut and submitted in an
AC CE P
imunofluorescence protocol for Fos protein. Key findings: The characterization tests indicated that βCP were efficiently incorporated into βCD. The oral treatment with βCPβCD, at all doses tested, produced a significant (p
S0024-3205(16)30099-6 doi: 10.1016/j.lfs.2016.02.049 LFS 14734
To appear in:
Life Sciences
Received date: Revised date: Accepted date:
25 October 2015 11 February 2016 11 February 2016
Please cite this article as: Quintans-J´ unior Lucindo J., Ara´ ujo Adriano A.S., Brito Renan G., Santos Priscila L., Quintans Jullyana S.S., Menezes Paula P., Serafini Mairim R., Silva Gabriel F., Carvalho Flavio M.S., Brogden Nicole K., Sluka Kathleen A., β-caryophyllene, a dietary cannabinoid, complexed with β-cyclodextrin produced anti-hyperalgesic effect involving the inhibition of Fos expression in superficial dorsal horn, Life Sciences (2016), doi: 10.1016/j.lfs.2016.02.049
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT β-caryophyllene, a dietary cannabinoid, complexed with β-cyclodextrin produced anti-hyperalgesic effect involving the inhibition of Fos expression in superficial
PT
dorsal horn
RI
Lucindo J. Quintans-Júnior1,3,#*, Adriano A.S. Araújo2,#, Renan G. Brito1,3, Priscila L.
SC
Santos1, Jullyana S.S. Quintans1, Paula P. Menezes2, Mairim R. Serafini2, Gabriel F.
2
Department of Physiology,
Department of Pharmacy.
3
Department of Food
MA
1
NU
Silva3, Flavio M.S. Carvalho4, Nicole K. Brogden5, Kathleen A. Sluka6
Engineering. Federal University of Sergipe, São Cristóvão, Sergipe, Brazil. Department of Mineralogy and Petrology, University of São Paulo, São Paulo, Brazil.
5
Department of Pharmaceutical Sciences and Experimental Therapeutics, College of
TE
D
4
Pharmacy, University of Iowa, Iowa City, Iowa, USA. Physical Therapy and Rehabilitation Science, Carver College of Medicine, University
AC CE P
6
of Iowa, Iowa City, Iowa, USA.
#
These authors contributed equally for the development of the paper.
*Author for correspondence: Lucindo J. Quintans-Júnior, Department of Physiology, Laboratory of Neuroscience and Pharmacological Assay. Federal University of Sergipe, Av. Marechal Rondon, s/n, São Cristóvão, Sergipe, Brazil (fax +55-79-2105-6640, Email: [email protected]; [email protected]).
Conflict of interest: The authors report no conflict of interest.
1
ACCEPTED MANUSCRIPT Abstract Aims: Evaluate the anti-hyperalgesic effect of the complex containing β-caryophyllene
PT
(βCP) and β-cyclodextrin (βCD) in a non-inflammatory chronic muscle pain mice model and investigated its action on superficial dorsal horn of the lumbar spinal cord.
RI
Main Methods: The βCP-βCD complex were prepared and characterized through the
SC
DSC, TG/DTG, FTIR, XRD and SEM. The model of chronic muscle pain was induced by two injections of pH 4.0 saline (20 µl) into left gastrocnemius 5 days apart. After
NU
confirming hyperalgesia, male mice were treated with βCP-βCD (10 or 20 mg/kg; p.o.)
MA
or vehicle (saline 0,9%, p.o.) daily for 9 days. 1 h after, the mechanical hyperalgesia, muscle withdrawal thresholds and motor performance were evaluated. To evaluate the βCP-βCD action on spinal cord, animals induced with chronic muscle pain were treated
TE
D
with βCP-βCD (20 mg/kg; p.o.) or vehicle (saline 0,9%, p.o.) and 90 min. after, were perfused, the lumbar spinal cord collected, crioprotected, cut and submitted in an
AC CE P
imunofluorescence protocol for Fos protein. Key findings: The characterization tests indicated that βCP were efficiently incorporated into βCD. The oral treatment with βCPβCD, at all doses tested, produced a significant (p
