The Journal of Rheumatology 14-3-3η Autoantibodies: Diagnostic Use in Early Rheumatoid Arthritis Walter P. Maksymowych, Gilles Boire, Dirkjan van Schaardenburg, Stephanie Wichuk, Samina Turk, Maarten Boers, Katherine A. Siminovitch, Vivian Bykerk, Ed Keystone, Paul Peter Tak, Arno W. van Kuijk, Robert Landewé, Desiree van der Heijde, Mairead Murphy and Anthony Marotta DOI: 10.3899/jrheum.141385 http://www.jrheum.org/content/early/2015/07/10/jrheum.141385 1. Sign up for our monthly e-table of contents http://www.jrheum.org/cgi/alerts/etoc 2. Information on Subscriptions http://jrheum.com/subscribe.html 3. Have us contact your library about access options [email protected] 4. Information on permissions/orders of reprints http://jrheum.com/reprints.html The Journal of Rheumatology is a monthly international serial edited by Earl D. Silverman featuring research articles on clinical subjects from scientists working in rheumatology and related fields.

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14-3-3η Autoantibodies: Diagnostic Use in Early Rheumatoid Arthritis

Walter­P.­Maksymowych,­Gilles­Boire,­Dirkjan­van­Schaardenburg,­Stephanie­Wichuk,­ Samina­Turk,­Maarten­Boers,­Katherine­A.­Siminovitch,­Vivian­Bykerk,­Ed­Keystone,­ Paul­Peter­Tak,­Arno­W.­van­Kuijk,­Robert­Landewé,­Desiree­van­der­Heijde,­Mairead­Murphy, and­Anthony­Marotta ABSTRACT. Objective. To­describe­the­expression­and­diagnostic­use­of­14-3-3η autoantibodies­in­early­rheumatoid arthritis­(RA). Methods. 14-3-3η autoantibody­levels­were­measured­using­an­electrochemiluminescent­multiplexed assay­in­500­subjects­(114­disease-modifying­antirheumatic­drug-naive­patients­with­early­RA,­135 with­established­RA,­55­healthy,­70­autoimmune,­and­126­other­non-RA­arthropathy­controls).­14-3-3η protein­levels­were­determined­in­an­earlier­analysis.­Two-tailed­Student­t­tests­and­Mann-Whitney­U tests­ compared­ differences­ among­ groups.­ Receiver-operator­ characteristic­ (ROC)­ curves­ were generated­and­diagnostic­performance­was­estimated­by­area­under­the­curve­(AUC),­as­well­as­specificity,­sensitivity,­and­likelihood­ratios­(LR)­for­optimal­cutoffs. Results. Median­serum­14-3-3η autoantibody­concentrations­were­significantly­higher­(p­