Proc. Natl. Acad. Sci. USA Vol. 88, pp. 10726-10729, December 1991 Medical Sciences

A broadly neutralizing monoclonal antibody that recognizes the V3 region of human immunodeficiency virus type 1 glycoprotein gpl20 TSUNEYA OHNO*t, MASAKI TERADA*, YUKIO YONEDA*, KEVIN W. SHEA*, REBECCA F. CHAMBERS*, DEBORAH M. STROKA*, MARIKO NAKAMURAt, AND DONALD W. KUFEt *Nissin Molecular Biology Institute, Inc., 20 Overland Street, Boston, MA 02215; tDepartment of Microbiology, tThe Jikei University School of Medicine, 3-25-8 Nishi Shinbashi Minatoku, Tokyo 105 Japan; and tThe Dana-Farber Capcer Institute, Harvard Medical School, Boston, MA 02115

Communicated by Michael H. Wigler, August 19, 1991

Previous studies have demonstrated that the ABSTRACT principal neutralizing determinant of human iunodeficiency virus type 1 (HIV-1) is located in the V3 loop of glycoprotein gpl20. Antibodies prepared against this region using gp120 or peptides as immunogens have been predonminantly HIV-1-isolate-specific. In the present studies, murine monoclonal antibodies (mAbs) were prepared against the HIV1MN strain. One mAb, designated NM-01, was selected for its ability to neutralize both the MN and TUB strains. Neutralization of H9-cell infectivity as determined by reverse transcriptase assay demonstrated an lDl5 of

A broadly neutralizing monoclonal antibody that recognizes the V3 region of human immunodeficiency virus type 1 glycoprotein gp120.

Previous studies have demonstrated that the principal neutralizing determinant of human immunodeficiency virus type 1 (HIV-1) is located in the V3 loo...
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