6ene. 87 (1990) 133-137 Elsevier
133
GENE03~3
A highly efficient electroporation system for transformation of Yersinia (Electric field pulse; gene transfer; Enterobacteriaceae: Y. enterocolitica; Y. pestis; Y. pseudotuberculosis)
Ram6n F. Conehas and Elisabeth Carniei Unitd d'Ecologie Bactd6enne, Institut Pasteur, Paris (France) Received by G. Bernardi: 8 August 1989 Revised: 20 September 1989 Accepted: 2 October 1989
SUMMARY
The various pathogenic Yersinia species are not readily and efficiently transformed by classical methods. For this reason, the electroporation technique was applied for genetic transformation of these species. Using optimal conditions, we were able to transform the six Yersinia strains studied with the two most widely used groups of plasmids: pSU2718 (a pACYCI84 derivative) and pKl9 (a pUCI9 derivative). Only Yersinia enterocolitica (Y. e.) serotype 0:8 gave poor results (
133
GENE03~3
A highly efficient electroporation system for transformation of Yersinia (Electric field pulse; gene transfer; Enterobacteriaceae: Y. enterocolitica; Y. pestis; Y. pseudotuberculosis)
Ram6n F. Conehas and Elisabeth Carniei Unitd d'Ecologie Bactd6enne, Institut Pasteur, Paris (France) Received by G. Bernardi: 8 August 1989 Revised: 20 September 1989 Accepted: 2 October 1989
SUMMARY
The various pathogenic Yersinia species are not readily and efficiently transformed by classical methods. For this reason, the electroporation technique was applied for genetic transformation of these species. Using optimal conditions, we were able to transform the six Yersinia strains studied with the two most widely used groups of plasmids: pSU2718 (a pACYCI84 derivative) and pKl9 (a pUCI9 derivative). Only Yersinia enterocolitica (Y. e.) serotype 0:8 gave poor results (
