213
Clinica Chimica Acta, 70 (1976) 213-217 @ Elsevier Scientific Publishing Company,
Amsterdam
- Printed in The Netherlands
BRIEF TECHNICAL NOTE CGA 7682
A METHOD FOR THE ESTIMATION OF 2-PHENYLETHYLAMINE IN HUMAN URINE BY GAS CHROMATOGRAPHY
G.P. REYNOLDS Department Kidderpore (Received
* and D-0. GRAY
of Botany and Biochemistry, Westfield Avenue, London NW3 7ST (U.K.) November
College (University
of London),
24,1976)
Summary A gas chromatographic procedure with flame ionisation detection has been developed to measure 2-phenylethylamine in human urine as the trifluoroacetyl derivative. The amine is purified by an ether extraction procedure and derivatised with trifluoroacetic anhydride. Quantification is obtained by comparing the peak height with that for an internal standard added to the urine before extraction. The normal 24-h excretion value for 2-phenylethylamine in human urine is found to be 6.8 * 2.9 pg (mean * S.D.). Introduction There is a developing interest in the biological activity of 2-phenylethylamine (phenethylamine, PE). PE has been implicated in the dietary triggering of migraine attacks [l] and there have been speculations that the compound is important in the aetiology of schizophrenia [2] and depression [3], as well as playing a role in normal brain function [4,5]. Abnormal levels of urinary PE have been reported in phenylketonuria [ 61, depression [ 71, schizophrenia [ 81 and cardiovascular disease [9]. Recent work has however cast doubt on previous methods of urinary PE quantification [lo] which were themselves inconsistent (Table I). Gas chromatography (GC) of perfluoroacyl derivatives has been widely and successfully used for the separation and analysis of biogenic amines [ 10-131. In this paper we present a simple and specific procedure for the extraction and quantification of urinary PE by GC of the trifluoroacetyl derivative. * To whom correspondence should be addressed.
214 TABLE I LEVELS JECTS PE in &24 Free
Clinica Chimica Acta, 70 (1976) 213-217 @ Elsevier Scientific Publishing Company,
Amsterdam
- Printed in The Netherlands
BRIEF TECHNICAL NOTE CGA 7682
A METHOD FOR THE ESTIMATION OF 2-PHENYLETHYLAMINE IN HUMAN URINE BY GAS CHROMATOGRAPHY
G.P. REYNOLDS Department Kidderpore (Received
* and D-0. GRAY
of Botany and Biochemistry, Westfield Avenue, London NW3 7ST (U.K.) November
College (University
of London),
24,1976)
Summary A gas chromatographic procedure with flame ionisation detection has been developed to measure 2-phenylethylamine in human urine as the trifluoroacetyl derivative. The amine is purified by an ether extraction procedure and derivatised with trifluoroacetic anhydride. Quantification is obtained by comparing the peak height with that for an internal standard added to the urine before extraction. The normal 24-h excretion value for 2-phenylethylamine in human urine is found to be 6.8 * 2.9 pg (mean * S.D.). Introduction There is a developing interest in the biological activity of 2-phenylethylamine (phenethylamine, PE). PE has been implicated in the dietary triggering of migraine attacks [l] and there have been speculations that the compound is important in the aetiology of schizophrenia [2] and depression [3], as well as playing a role in normal brain function [4,5]. Abnormal levels of urinary PE have been reported in phenylketonuria [ 61, depression [ 71, schizophrenia [ 81 and cardiovascular disease [9]. Recent work has however cast doubt on previous methods of urinary PE quantification [lo] which were themselves inconsistent (Table I). Gas chromatography (GC) of perfluoroacyl derivatives has been widely and successfully used for the separation and analysis of biogenic amines [ 10-131. In this paper we present a simple and specific procedure for the extraction and quantification of urinary PE by GC of the trifluoroacetyl derivative. * To whom correspondence should be addressed.
214 TABLE I LEVELS JECTS PE in &24 Free
