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A novel HLA class II allele, HLA-DQB1*03:73 J. W. Zheng
| J. H. Xie | Y. Sun | Y. Q. Jiang | D. Xiang
Blood Typing Laboratory, Shanghai Blood Center, Shanghai, China Correspondence Dong Xiang, PhD, Blood Typing Laboratory, Shanghai Blood Center, No. 1191, HongQiao Road, ChangNing, Shanghai 200051, China. Email:
[email protected] The HLA-DQB1*03:73 allele differs from DQB1*03:01:01:01 by 1 nucleotide substitution at position 209. KEYWORDS
HLA-DQB1*03:73, new allele, sequence-based typing
The human leukocyte antigen (HLA) genes are amongst the most polymorphic in the human genome. In recent years, the number of sequences in the database increased on average by 29% each year.1 To date, 16 755 HLA alleles including those of 1079 HLA-DQB1 alleles have been reported according to IPD-IMGT/HLA database (release 3.28.0, April 2017). Now, we report a novel allele HLA-DQB1*03:73 identified from a registered donor of China Marrow Donor Program (CMDP). In our laboratory, HLA sequence-based typing (SBT) is the routine typing approach. We used DNA sequencing reagents (SeCore SBT Kits, Invitrogen, Madison, Wisconsin) run on the ABI 3730xl Genetic Analyzer (Applied Biosystems, Foster City, California), and analyzed with uTYPE6.0 analysis software (Invitrogen, Carlsbad, California). The sample originally was typed as HLA-A*02:01, 02:07; B*40:02, 46:01; DRB1*11:01, 12:02; C*01:02, 03:04; DQB1*03:01, 03:01v. To confirm the potentially novel allele, supplemental tests were applied using HLAssure SBT Typing KIT (Texas BioGene Inc, New Taipei City, Taiwan), which selectively amplified DQB1*03 by group-specific primers to accurately detect the 1 novel allele. All sequencing assays were repeated
3 times from independent PCR amplifications and the results were shown to be identical. Nucleotide sequence alignments with HLA-DQB1 alleles from the IPD-IMGT/HLA sequence database showed that the exon 2 sequence of the novel allele was identical to HLADQB1*03:01:01:01, except for 1 nucleotide change at nucleotide 209 where C>T (codon 38 GCA>GTA) (Figure 1), resulting in a coding change, Alanine 38 is changed to Valine. The nucleotide sequence is available at GenBank accession number KC970435. The new allele has been officially named DQB1*03:73 by the World Health Organization (WHO) Nomenclature Committee for factors of the HLA system in April 2013. This follows the agreed policy that subject to the conditions stated in the most recent Nomenclature Report,1 names will be assigned to new sequences as they are identified. The list of such new names will be published in the following WHO Nomenclature Report.
ACKNOWLEDGMENTS
This work was funded by the Public Health Academic Project awarded by Shanghai Municipal Health Bureau.
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Alignment of the sequence of exon 2 of DQB1*03:73 with the sequence of DQB1*03:01:01:01. Dashes indicate nucleotide identity with the DQB1*03:01:01:01 allele sequence. Numbers above the sequence correspond to codon position
FIGURE 1
Conflict of interest The authors have declared no conflicting interests. REFERENC E 1. Marsh SGE. Nomenclature for factors of the HLA system, update April 2013. Tissue Antigens. 2013;82:219-223.
How to cite this article: Zheng JW, Xie JH, Sun Y, Jiang YQ, Xiang D. A novel HLA class II allele, HLA-DQB1*03:73. HLA. 2017;90:261–262. https:// doi.org/10.1111/tan.13083