HHS Public Access Author manuscript Author Manuscript
Analyst. Author manuscript; available in PMC 2017 September 19. Published in final edited form as: Analyst. 2016 February 07; 141(3): 947–955. doi:10.1039/c5an01969a.
A simple integrated microfluidic device for the multiplexed fluorescence-free detection of Salmonella enterica Briony C. Strachana,†, Hillary S. Sloanea,†, Eric Houptb, Jacob C. Leea, Daniel C. Miraniana, Jingyi Lia, Daniel A. Nelsona, and James P. Landersa,c,d,* aDept
of Chemistry, McCormick Road, University of Virginia, Charlottesville, VA 22904, USA
Author Manuscript
bDept
of Infectious Disease, University of Virginia Health Science Center, Charlottesville, VA 22908, USA
cDept
of Pathology, University of Virginia Health Science Center, Charlottesville, VA 22908, USA
dDept
of Mechanical Engineering, University of Virginia, Engineer’s Way, Charlottesville, VA 22904, USA
Abstract
Author Manuscript
Rapid, inexpensive and simplistic nucleic acid testing (NAT) is pivotal in delivering biotechnology solutions at the point-of-care (POC). We present a poly(methylmethacrylate) (PMMA) microdevice where on-board infrared-mediated PCR amplification is seamlessly integrated with a particle-based, visual DNA detection for specific detection of bacterial targets in less than 35 minutes. Fluidic control is achieved using a capillary burst valve laser-ablated in a novel manner to confine the PCR reagents to a chamber during thermal cycling, and a manual torque-actuated pressure system to mobilize the fluid from the PCR chamber to the detection reservoir containing oligonucleotide-adducted magnetic particles. Interaction of amplified products specific to the target organism with the beads in a rotating magnetic field allows for near instantaneous (
Analyst. Author manuscript; available in PMC 2017 September 19. Published in final edited form as: Analyst. 2016 February 07; 141(3): 947–955. doi:10.1039/c5an01969a.
A simple integrated microfluidic device for the multiplexed fluorescence-free detection of Salmonella enterica Briony C. Strachana,†, Hillary S. Sloanea,†, Eric Houptb, Jacob C. Leea, Daniel C. Miraniana, Jingyi Lia, Daniel A. Nelsona, and James P. Landersa,c,d,* aDept
of Chemistry, McCormick Road, University of Virginia, Charlottesville, VA 22904, USA
Author Manuscript
bDept
of Infectious Disease, University of Virginia Health Science Center, Charlottesville, VA 22908, USA
cDept
of Pathology, University of Virginia Health Science Center, Charlottesville, VA 22908, USA
dDept
of Mechanical Engineering, University of Virginia, Engineer’s Way, Charlottesville, VA 22904, USA
Abstract
Author Manuscript
Rapid, inexpensive and simplistic nucleic acid testing (NAT) is pivotal in delivering biotechnology solutions at the point-of-care (POC). We present a poly(methylmethacrylate) (PMMA) microdevice where on-board infrared-mediated PCR amplification is seamlessly integrated with a particle-based, visual DNA detection for specific detection of bacterial targets in less than 35 minutes. Fluidic control is achieved using a capillary burst valve laser-ablated in a novel manner to confine the PCR reagents to a chamber during thermal cycling, and a manual torque-actuated pressure system to mobilize the fluid from the PCR chamber to the detection reservoir containing oligonucleotide-adducted magnetic particles. Interaction of amplified products specific to the target organism with the beads in a rotating magnetic field allows for near instantaneous (
