Funct Integr Genomics (2014) 14:717–730 DOI 10.1007/s10142-014-0394-z
ORIGINAL PAPER
ABI-like transcription factor gene TaABL1 from wheat improves multiple abiotic stress tolerances in transgenic plants Dong-Bei Xu & Shi-Qing Gao & You-Zhi Ma & Zhao-Shi Xu & Chang-Ping Zhao & Yi-Miao Tang & Xue-Yin Li & Lian-Cheng Li & Yao-Feng Chen & Ming Chen
Received: 16 March 2014 / Revised: 11 August 2014 / Accepted: 19 August 2014 / Published online: 25 October 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract The phytohormone abscisic acid (ABA) plays crucial roles in adaptive responses of plants to abiotic stresses. ABA-responsive element binding proteins (AREBs) are basic leucine zipper transcription factors that regulate the expression of downstream genes containing ABA-responsive elements (ABREs) in promoter regions. A novel ABI-like (ABAinsensitive) transcription factor gene, named TaABL1, containing a conserved basic leucine zipper (bZIP) domain was cloned from wheat. Southern blotting showed that three copies were present in the wheat genome. Phylogenetic analyses indicated that TaABL1 belonged to the AREB subfamily of the bZIP transcription factor family and was most closely related to ZmABI5 in maize and OsAREB2 in rice. Expression of TaABL1 was highly induced in wheat roots, stems, and leaves
by ABA, drought, high salt, and low temperature stresses. TaABL1 was localized inside the nuclei of transformed wheat mesophyll protoplast. Overexpression of TaABL1 enhanced responses of transgenic plants to ABA and hastened stomatal closure under stress, thereby improving tolerance to multiple abiotic stresses. Furthermore, overexpression of TaABL1 upregulated or downregulated the expression of some stressrelated genes controlling stomatal closure in transgenic plants under ABA and drought stress conditions, suggesting that TaABL1 might be a valuable genetic resource for transgenic molecular breeding. Keywords Triticum aestivum L. . bZIP transcription factor . ABA . Drought . High salt . Low temperature
Dong-Bei Xu and Shi-Qing Gao contributed equally to this work. Electronic supplementary material The online version of this article (doi:10.1007/s10142-014-0394-z) contains supplementary material, which is available to authorized users. D.
ORIGINAL PAPER
ABI-like transcription factor gene TaABL1 from wheat improves multiple abiotic stress tolerances in transgenic plants Dong-Bei Xu & Shi-Qing Gao & You-Zhi Ma & Zhao-Shi Xu & Chang-Ping Zhao & Yi-Miao Tang & Xue-Yin Li & Lian-Cheng Li & Yao-Feng Chen & Ming Chen
Received: 16 March 2014 / Revised: 11 August 2014 / Accepted: 19 August 2014 / Published online: 25 October 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract The phytohormone abscisic acid (ABA) plays crucial roles in adaptive responses of plants to abiotic stresses. ABA-responsive element binding proteins (AREBs) are basic leucine zipper transcription factors that regulate the expression of downstream genes containing ABA-responsive elements (ABREs) in promoter regions. A novel ABI-like (ABAinsensitive) transcription factor gene, named TaABL1, containing a conserved basic leucine zipper (bZIP) domain was cloned from wheat. Southern blotting showed that three copies were present in the wheat genome. Phylogenetic analyses indicated that TaABL1 belonged to the AREB subfamily of the bZIP transcription factor family and was most closely related to ZmABI5 in maize and OsAREB2 in rice. Expression of TaABL1 was highly induced in wheat roots, stems, and leaves
by ABA, drought, high salt, and low temperature stresses. TaABL1 was localized inside the nuclei of transformed wheat mesophyll protoplast. Overexpression of TaABL1 enhanced responses of transgenic plants to ABA and hastened stomatal closure under stress, thereby improving tolerance to multiple abiotic stresses. Furthermore, overexpression of TaABL1 upregulated or downregulated the expression of some stressrelated genes controlling stomatal closure in transgenic plants under ABA and drought stress conditions, suggesting that TaABL1 might be a valuable genetic resource for transgenic molecular breeding. Keywords Triticum aestivum L. . bZIP transcription factor . ABA . Drought . High salt . Low temperature
Dong-Bei Xu and Shi-Qing Gao contributed equally to this work. Electronic supplementary material The online version of this article (doi:10.1007/s10142-014-0394-z) contains supplementary material, which is available to authorized users. D.
