Vol. 182, No. 2, 1992 January 31, 1992
Alkaline
BIOCHEMICALAND
BIOPHYSICALRESEARCH
Phosphatase Activity during Promyelocytic Leukemia
Lillian L-L.Weil,Eric
Differentiation of the Human Cell Line, HL-60
K-W. HuiZ,J.S.Weil
1 School of Medical Technology
and
November
20,
Y-M. Yungz*
Benjamin
and 2 Department
Chang Gung Medical College,
Received
COMMUNICATIONS Pages 487-494
of Pharmacology,
Taiwan,
R.O.C.
1991
ABSTRACT The differentiation mature myelocytic cells induced by a quantitative
similar increase
The potentiation
of RA-induced
of HL-60 promyelocytic cells toward by retinoic acid (RA) was accompanied in alkaline phosphatase differentiation
ALP activity
by sphinganine
seemed
combination
of RA and sphinganine
(ALP) activity.
and the enhancement
to correlate
of
with each other.
The
increased in parallel the percentage Short exposures (4-8h) of HL-60 of mature cells and the ALP activities. cells to RA promoted differentiation and ALP activity to a fraction (about 50%) of their maximums
which were achieved in cells after 24h or longer
RA exposure. Our results indicate ALP activity and the differentiation 24h of incubation.
Alkaline isozymes
Q 1992 Academic
phosphatases that
proteins
that
inositol
anchor
the enzyme
(2).
family
the
to the cellular
The physiological
knowledge in various
ALPS) comprise
hydrolysis
at an alkaline pH (1).
are bound
spite of thorough
P,,,,, lnC.
(EC 3.1.3.1.,
catalyzes
phosphomonoesters
that the key step for the induction of by RA probably takes place within
of
a
wide
a group
of
variety
of
They belong to a category membrane
function
animal species
via a phosphatidyl-
of ALP is not known,
of the gene structure (3-6).
of
of many members Nevertheless,
in of
it has
*
To whom reprint requests should be addressed at Dept. of Pharmacology, Chang Gung Medical College, 259 Wen-Hwa 1st Rd, Kwei-San, Tao-Yuan 333, Taiwan, R.O.C. 0006-291X/92 487
All
$1.50
Copyright 0 1992 by Academic Press, Inc, rights of reproduction in any form reserved.
Vol.
182,
No.
2, 1992
been
BIOCHEMICAL
suggested
differentiation
that
these
(7-I I).
adenocarcinoma
and
correspondingly appearance
that
cells
exhibit rates
recently
been shown
(7).
The promyelocytic capacity (12). cells
to differentiate The extent
is known
differentiations respectively
to
with increases
be dose-
differentiation
(13).
(13,141.
modulated
RA-treated
with which and induction
(13).
to study the relationship of differentiation.
and time-dependent
changes
between
of HL-60
cells can
sphinganine
a good in vitro
the increased
This communication in ALP activity
(2h) of HL-
of its maximal
by a protein kinase C inhibitor,
HL-60 cells thus constitute
HL-60
of 1.0 81 IOpM
(
Alkaline
BIOCHEMICALAND
BIOPHYSICALRESEARCH
Phosphatase Activity during Promyelocytic Leukemia
Lillian L-L.Weil,Eric
Differentiation of the Human Cell Line, HL-60
K-W. HuiZ,J.S.Weil
1 School of Medical Technology
and
November
20,
Y-M. Yungz*
Benjamin
and 2 Department
Chang Gung Medical College,
Received
COMMUNICATIONS Pages 487-494
of Pharmacology,
Taiwan,
R.O.C.
1991
ABSTRACT The differentiation mature myelocytic cells induced by a quantitative
similar increase
The potentiation
of RA-induced
of HL-60 promyelocytic cells toward by retinoic acid (RA) was accompanied in alkaline phosphatase differentiation
ALP activity
by sphinganine
seemed
combination
of RA and sphinganine
(ALP) activity.
and the enhancement
to correlate
of
with each other.
The
increased in parallel the percentage Short exposures (4-8h) of HL-60 of mature cells and the ALP activities. cells to RA promoted differentiation and ALP activity to a fraction (about 50%) of their maximums
which were achieved in cells after 24h or longer
RA exposure. Our results indicate ALP activity and the differentiation 24h of incubation.
Alkaline isozymes
Q 1992 Academic
phosphatases that
proteins
that
inositol
anchor
the enzyme
(2).
family
the
to the cellular
The physiological
knowledge in various
ALPS) comprise
hydrolysis
at an alkaline pH (1).
are bound
spite of thorough
P,,,,, lnC.
(EC 3.1.3.1.,
catalyzes
phosphomonoesters
that the key step for the induction of by RA probably takes place within
of
a
wide
a group
of
variety
of
They belong to a category membrane
function
animal species
via a phosphatidyl-
of ALP is not known,
of the gene structure (3-6).
of
of many members Nevertheless,
in of
it has
*
To whom reprint requests should be addressed at Dept. of Pharmacology, Chang Gung Medical College, 259 Wen-Hwa 1st Rd, Kwei-San, Tao-Yuan 333, Taiwan, R.O.C. 0006-291X/92 487
All
$1.50
Copyright 0 1992 by Academic Press, Inc, rights of reproduction in any form reserved.
Vol.
182,
No.
2, 1992
been
BIOCHEMICAL
suggested
differentiation
that
these
(7-I I).
adenocarcinoma
and
correspondingly appearance
that
cells
exhibit rates
recently
been shown
(7).
The promyelocytic capacity (12). cells
to differentiate The extent
is known
differentiations respectively
to
with increases
be dose-
differentiation
(13).
(13,141.
modulated
RA-treated
with which and induction
(13).
to study the relationship of differentiation.
and time-dependent
changes
between
of HL-60
cells can
sphinganine
a good in vitro
the increased
This communication in ALP activity
(2h) of HL-
of its maximal
by a protein kinase C inhibitor,
HL-60 cells thus constitute
HL-60
of 1.0 81 IOpM
(
