Short Communications 436
Altered Cellular Distribution of Hexokinase in Skeletal Muscle after Exercise D. R. Van Houten ', J. M Davis, D. M Meyers, J. L. Durstine Department of Exercise Science, Exercise Biochemistry Laboratory, University of South Carolina, Columbia, South Carolina 29208
D. R. Van Houten, J. M Davis, D. M Meyers andf. L. Durstine, Altered Cellular Distribution of Hexokinase in Skeletal Muscle after Exercise. mt J Sports Mcd, Vol l3,No5,pp436—438, 1992.
fluenced by its distribution between the mitochondrial-bound and soluble forms (2, 11). The binding of HK to mitochondria can occur in the brain during changes of the in vivo energy sta-
tus (4) and in mitochondrial preparations obtained from skeletal muscle as the intracellular concentrations of ATP and glucose-6-phosphate are altered (13).
Accepted: February 28, 1992
Presently, it is not known whether the intracellular distribution of HK is affected by physical exercise.
The purpose of this study was to determine
This is in spite of the fact that physical exercise has been shown to increase the activity of total HK in skeletal muscle (10), and
the effects of a single period of swim exercise to exhaustion
it has been proposed that the insulin-like effect of exercise
on the cellular distribution of hexokinase in rat skeletal muscle. The gastrocnemius muscle from male rats exercised (n 16) to exhaustion or allowed to remain inactive (n = 14) was analyzed for hexokinase activity (HK) in the whole muscle homogenate and the mitochondrial-rich fraction. Although exercise produced a slight, non-significant,
increase in HK activity in the whole muscle homogenate (p
Altered Cellular Distribution of Hexokinase in Skeletal Muscle after Exercise D. R. Van Houten ', J. M Davis, D. M Meyers, J. L. Durstine Department of Exercise Science, Exercise Biochemistry Laboratory, University of South Carolina, Columbia, South Carolina 29208
D. R. Van Houten, J. M Davis, D. M Meyers andf. L. Durstine, Altered Cellular Distribution of Hexokinase in Skeletal Muscle after Exercise. mt J Sports Mcd, Vol l3,No5,pp436—438, 1992.
fluenced by its distribution between the mitochondrial-bound and soluble forms (2, 11). The binding of HK to mitochondria can occur in the brain during changes of the in vivo energy sta-
tus (4) and in mitochondrial preparations obtained from skeletal muscle as the intracellular concentrations of ATP and glucose-6-phosphate are altered (13).
Accepted: February 28, 1992
Presently, it is not known whether the intracellular distribution of HK is affected by physical exercise.
The purpose of this study was to determine
This is in spite of the fact that physical exercise has been shown to increase the activity of total HK in skeletal muscle (10), and
the effects of a single period of swim exercise to exhaustion
it has been proposed that the insulin-like effect of exercise
on the cellular distribution of hexokinase in rat skeletal muscle. The gastrocnemius muscle from male rats exercised (n 16) to exhaustion or allowed to remain inactive (n = 14) was analyzed for hexokinase activity (HK) in the whole muscle homogenate and the mitochondrial-rich fraction. Although exercise produced a slight, non-significant,
increase in HK activity in the whole muscle homogenate (p
