Journal of
Lasers
in Medical Sciences
Original Article
J Lasers Med Sci 2016 Winter;7(1):21-25 http://www.journals.sbmu.ac.ir/jlms
doi 10.15171/jlms.2016.05
An In Vitro Comparison of Antimicrobial Effects of Curcumin-Based Photodynamic Therapy and Chlorhexidine, on Aggregatibacter actinomycetemcomitans Shamsoulmolouk Najafi1,2, Mina Khayamzadeh1, Mojgan Paknejad3, Golfam Poursepanj4, Mohammad Javad Kharazi Fard4, Abbas Bahador5* Department of Oral Medicine, International Campus, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran 2 Department of Dental Research Center and Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran 3 Department of Periodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. 4 International Campus, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran 5 Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 1
*Correspondence to
Abbas Bahador, PhD; Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Tel: +982164053210; Fax: +982188955810; Email: [email protected]
Published online 7 January 2016
Abstract Introduction: Considering the importance of prevention in periodontal diseases and the important role of Aggregatibacter actinomycetemcomitans in induction and progression of these diseases, the aim of the present in vitro study was to compare the antimicrobial effects of chlorhexidine digluconate (CHX), curcumin and light-emitting diode (LED) laser, on this bacterium. Methods: Antimicrobial activity of curcumin (5 mg/ml), CHX (2%), LED (120 J/cm2) and LED + curcumin (120 J/cm2 + 2.5 mg/ml) against A. actinomycetemcomitans were tested in vitro, using micro-broth dilution test. One-way analysis of variance (ANOVA) and Tukey’s HSD tests served for statistical analysis. Results: Regarding the minimum inhibitory concentration (MIC), CHX had a significantly lower MIC than curcumin (P
Lasers
in Medical Sciences
Original Article
J Lasers Med Sci 2016 Winter;7(1):21-25 http://www.journals.sbmu.ac.ir/jlms
doi 10.15171/jlms.2016.05
An In Vitro Comparison of Antimicrobial Effects of Curcumin-Based Photodynamic Therapy and Chlorhexidine, on Aggregatibacter actinomycetemcomitans Shamsoulmolouk Najafi1,2, Mina Khayamzadeh1, Mojgan Paknejad3, Golfam Poursepanj4, Mohammad Javad Kharazi Fard4, Abbas Bahador5* Department of Oral Medicine, International Campus, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran 2 Department of Dental Research Center and Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran 3 Department of Periodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. 4 International Campus, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran 5 Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 1
*Correspondence to
Abbas Bahador, PhD; Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Tel: +982164053210; Fax: +982188955810; Email: [email protected]
Published online 7 January 2016
Abstract Introduction: Considering the importance of prevention in periodontal diseases and the important role of Aggregatibacter actinomycetemcomitans in induction and progression of these diseases, the aim of the present in vitro study was to compare the antimicrobial effects of chlorhexidine digluconate (CHX), curcumin and light-emitting diode (LED) laser, on this bacterium. Methods: Antimicrobial activity of curcumin (5 mg/ml), CHX (2%), LED (120 J/cm2) and LED + curcumin (120 J/cm2 + 2.5 mg/ml) against A. actinomycetemcomitans were tested in vitro, using micro-broth dilution test. One-way analysis of variance (ANOVA) and Tukey’s HSD tests served for statistical analysis. Results: Regarding the minimum inhibitory concentration (MIC), CHX had a significantly lower MIC than curcumin (P
