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Analysis of breath volatile organic compounds in children with chronic liver disease compared to healthy controls
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2015 J. Breath Res. 9 026002 (http://iopscience.iop.org/1752-7163/9/2/026002) View the table of contents for this issue, or go to the journal homepage for more
Download details: IP Address: 130.194.20.173 This content was downloaded on 05/05/2015 at 01:33
Please note that terms and conditions apply.
IOP Publishing
J. Breath Res. 9 (2015) 026002
doi:10.1088/1752-7155/9/2/026002
Paper
received
1 August 2014
Analysis of breath volatile organic compounds in children with chronic liver disease compared to healthy controls
re vised
14 November 2014 accep ted for publication
2 December 2014 published
20 April 2015
Katharine Eng1, Naim Alkhouri1,2, Frank Cikach2,3, Nishaben Patel1, Chen Yan1, David Grove3,4, Rocio Lopez5, Ellen Rome6 and Raed A Dweik3,4 1
3 4 5 6 2
Department of Pediatric Gastroenterology and Hepatology, Cleveland Clinic, Cleveland, OH, USA Digestive Disease Institute, Cleveland Clinic, Cleveland, OH, USA Department of Pulmonary, Allergy and Critical Care Medicine, Respiratory Institute, Cleveland Clinic, Cleveland, OH, USA Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA Department of Quantitative Health Sciences, Cleveland, Clinic, Cleveland, OH, USA Department of General Pediatrics, Cleveland Clinic, Cleveland, OH, USA
E-mail: [email protected] Keywords: breath testing, pediatric, chronic liver disease, biomarker, volatile organic compounds
Abstract Breath testing is increasingly being used as a non-invasive diagnostic tool for disease states across medicine. The purpose of this study was to compare the levels of volatile organic compounds (VOCs) as measured by mass spectrometry in healthy children and children with chronic liver disease (CLD). Patients between the ages of 6 and 21 were recruited for the study. Control subjects were recruited from a general pediatric population during well-child visits, while patients with CLD were recruited from pediatric gastroenterology clinic visits. The diagnosis of CLD was confirmed by clinical, laboratory, and/or histologic data. A single exhaled breath was collected and analyzed by means of selected-ion flow-tube mass spectrometry per protocol. A total of 104 patients were included in the study (49 with CLD and 55 healthy controls). Of the patients with CLD, 20 had advanced liver fibrosis (F3–F4). In the CLD cohort, levels of exhaled 1-decene, 1-heptene, 1-octene and 3 methylhexane were found to be significantly higher when compared to the control population (p
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My IOPscience
Analysis of breath volatile organic compounds in children with chronic liver disease compared to healthy controls
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2015 J. Breath Res. 9 026002 (http://iopscience.iop.org/1752-7163/9/2/026002) View the table of contents for this issue, or go to the journal homepage for more
Download details: IP Address: 130.194.20.173 This content was downloaded on 05/05/2015 at 01:33
Please note that terms and conditions apply.
IOP Publishing
J. Breath Res. 9 (2015) 026002
doi:10.1088/1752-7155/9/2/026002
Paper
received
1 August 2014
Analysis of breath volatile organic compounds in children with chronic liver disease compared to healthy controls
re vised
14 November 2014 accep ted for publication
2 December 2014 published
20 April 2015
Katharine Eng1, Naim Alkhouri1,2, Frank Cikach2,3, Nishaben Patel1, Chen Yan1, David Grove3,4, Rocio Lopez5, Ellen Rome6 and Raed A Dweik3,4 1
3 4 5 6 2
Department of Pediatric Gastroenterology and Hepatology, Cleveland Clinic, Cleveland, OH, USA Digestive Disease Institute, Cleveland Clinic, Cleveland, OH, USA Department of Pulmonary, Allergy and Critical Care Medicine, Respiratory Institute, Cleveland Clinic, Cleveland, OH, USA Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA Department of Quantitative Health Sciences, Cleveland, Clinic, Cleveland, OH, USA Department of General Pediatrics, Cleveland Clinic, Cleveland, OH, USA
E-mail: [email protected] Keywords: breath testing, pediatric, chronic liver disease, biomarker, volatile organic compounds
Abstract Breath testing is increasingly being used as a non-invasive diagnostic tool for disease states across medicine. The purpose of this study was to compare the levels of volatile organic compounds (VOCs) as measured by mass spectrometry in healthy children and children with chronic liver disease (CLD). Patients between the ages of 6 and 21 were recruited for the study. Control subjects were recruited from a general pediatric population during well-child visits, while patients with CLD were recruited from pediatric gastroenterology clinic visits. The diagnosis of CLD was confirmed by clinical, laboratory, and/or histologic data. A single exhaled breath was collected and analyzed by means of selected-ion flow-tube mass spectrometry per protocol. A total of 104 patients were included in the study (49 with CLD and 55 healthy controls). Of the patients with CLD, 20 had advanced liver fibrosis (F3–F4). In the CLD cohort, levels of exhaled 1-decene, 1-heptene, 1-octene and 3 methylhexane were found to be significantly higher when compared to the control population (p
