doi: 10.1111/jop.12212
J Oral Pathol Med © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd wileyonlinelibrary.com/journal/jop
ANGPTL4 regulates the metastatic potential of oral squamous cell carcinoma Junichi Tanaka, Tarou Irie, Gou Yamamoto, Rika Yasuhara, Tomohide Isobe, Chie Hokazono, Tetsuhiko Tachikawa, Yohko Kohno, Kenji Mishima Division of Pathology, Department of Oral Diagnostic Sciences, School of Dentistry, Showa University, Shinagawa-ku, Tokyo, Japan
Lymph node metastasis is a major factor for poor prognosis in oral squamous cell carcinoma (OSCC). However, the molecular mechanisms of lymph node metastasis are unclear. We determined that angiopoietin-like protein 4 (ANGPTL4) mRNA and protein expression were increased in OSCC cells established from the primary site in metastatic cases. In addition, ANGPTL4 expression in biopsy specimens was correlated with the presence of lymph node metastasis. Therefore, our initial findings suggest that OSCC cells expressing ANGPTL4 may possess metastatic ability. Furthermore, cell culture supernatants from OSCC cells that metastasized to the lymph node contain ANGPTL4 and promote invasive ability. These findings suggest that secreted ANGPTL4 may affect the invasive ability of OSCC. Moreover, the rates of positive ANGPTL4 expression at the primary site were significantly higher in the lymph node metastasis group. These results demonstrate that ANGPTL4 contributes to OSCC metastasis by stimulating cell invasion. Therefore, ANGPTL4 is a potential therapeutic target for preventing cancer metastasis. J Oral Pathol Med (2014) Keywords: angiopoietin-like protein 4; cancer therapy; invasion; lymph node metastasis; oral squamous cell carcinoma
Introduction Oral squamous cell carcinoma (OSCC) is the most common head and neck neoplasm and the 10th most diagnosed cancer worldwide (1–3). The prognosis of advanced cases is poor, and 5-year survival rates of OSCC are
J Oral Pathol Med © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd wileyonlinelibrary.com/journal/jop
ANGPTL4 regulates the metastatic potential of oral squamous cell carcinoma Junichi Tanaka, Tarou Irie, Gou Yamamoto, Rika Yasuhara, Tomohide Isobe, Chie Hokazono, Tetsuhiko Tachikawa, Yohko Kohno, Kenji Mishima Division of Pathology, Department of Oral Diagnostic Sciences, School of Dentistry, Showa University, Shinagawa-ku, Tokyo, Japan
Lymph node metastasis is a major factor for poor prognosis in oral squamous cell carcinoma (OSCC). However, the molecular mechanisms of lymph node metastasis are unclear. We determined that angiopoietin-like protein 4 (ANGPTL4) mRNA and protein expression were increased in OSCC cells established from the primary site in metastatic cases. In addition, ANGPTL4 expression in biopsy specimens was correlated with the presence of lymph node metastasis. Therefore, our initial findings suggest that OSCC cells expressing ANGPTL4 may possess metastatic ability. Furthermore, cell culture supernatants from OSCC cells that metastasized to the lymph node contain ANGPTL4 and promote invasive ability. These findings suggest that secreted ANGPTL4 may affect the invasive ability of OSCC. Moreover, the rates of positive ANGPTL4 expression at the primary site were significantly higher in the lymph node metastasis group. These results demonstrate that ANGPTL4 contributes to OSCC metastasis by stimulating cell invasion. Therefore, ANGPTL4 is a potential therapeutic target for preventing cancer metastasis. J Oral Pathol Med (2014) Keywords: angiopoietin-like protein 4; cancer therapy; invasion; lymph node metastasis; oral squamous cell carcinoma
Introduction Oral squamous cell carcinoma (OSCC) is the most common head and neck neoplasm and the 10th most diagnosed cancer worldwide (1–3). The prognosis of advanced cases is poor, and 5-year survival rates of OSCC are
