ANIMAL MODEL OF HUMAN DISEASE I~~~~~~~~~
Diabetes Mlellitus
Animal Model: Sekoke: Diabetes of Nutritional Origin in Carp
Contributed by: Stewart Wolf, MD, Director of the Marine Biomedical Institute, University of Texas Medical Branch, Galveston, Texas 77550. Biologc Featur
Sekoke is a wasting disease identified by Japanese workers among carp in commercial fish farms.1 It has been found that the causative agent was nutritional, an excess of oxidized unsaturated fatty acids in the diet derived from feeding silkworm pupae.2'3 The investigators were able to induce the disease experimentally in carp by feeding a diet containing 10% oxidized saurv oil for 60 days, and could prevent the disease by prophylactic treatment with a-tocopherol. The "clinical" features of the disease include hyperglycemia and glycosuria that are relatively insulin resistant, decreased glucose tolerance, and occasionally, ketonuna. The lesions include most prominently a waxy degeneration of skeletal muscles 4 and a vascular proliferative retinopathy.5 Yokote, who made extensive histologic studies, demonstrated lesions of the pancreatic islets, the renal glomeruli (Figure 1), vitreal vessels (Figure 2), and peripheral nerves (Figure 3) that closely resemble those found in human diabetes.6 In addition, he observed defective gonadal development in affected carp of both sexes. Yokote compared the pathologic findings in Sekoke with diabetes induced in carp by alloxan 7 and hvdrocortisone.8 The former induced aB-cell degranulation in the pancreas and hyperglycemia together with muscle and glomerular lesions but no retinal lesions, in contrast to 2 weeks of hvdrocortisone injections, which reproduced most of the pathologic picture of Sekoke. Potential Usefuness of the Mod
The ability to produce a disease closely resembling human juvenile diabetes mellitus both clinically and histologically by dietarv manipulation offers far better flexibility to the experimenter than do pancreatic Publication sponsored by the Registry of Comparative Pathology of the Armed Forces Institute of b% Public Health Service Grant RR 00301 from the Division of Research Resources. US Department of Health. Education and Welfare. under the auspices of Universities Associated for Research and Education in Pathology. Inc All illustrations are bv Dr. M1otovoshi Yokote of the Freshwater Fisheries Research Laborator^Kino. Tokyo. Japan, and are published by his permission. 805
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Diabetes Mlellitus
Animal Model: Sekoke: Diabetes of Nutritional Origin in Carp
Contributed by: Stewart Wolf, MD, Director of the Marine Biomedical Institute, University of Texas Medical Branch, Galveston, Texas 77550. Biologc Featur
Sekoke is a wasting disease identified by Japanese workers among carp in commercial fish farms.1 It has been found that the causative agent was nutritional, an excess of oxidized unsaturated fatty acids in the diet derived from feeding silkworm pupae.2'3 The investigators were able to induce the disease experimentally in carp by feeding a diet containing 10% oxidized saurv oil for 60 days, and could prevent the disease by prophylactic treatment with a-tocopherol. The "clinical" features of the disease include hyperglycemia and glycosuria that are relatively insulin resistant, decreased glucose tolerance, and occasionally, ketonuna. The lesions include most prominently a waxy degeneration of skeletal muscles 4 and a vascular proliferative retinopathy.5 Yokote, who made extensive histologic studies, demonstrated lesions of the pancreatic islets, the renal glomeruli (Figure 1), vitreal vessels (Figure 2), and peripheral nerves (Figure 3) that closely resemble those found in human diabetes.6 In addition, he observed defective gonadal development in affected carp of both sexes. Yokote compared the pathologic findings in Sekoke with diabetes induced in carp by alloxan 7 and hvdrocortisone.8 The former induced aB-cell degranulation in the pancreas and hyperglycemia together with muscle and glomerular lesions but no retinal lesions, in contrast to 2 weeks of hvdrocortisone injections, which reproduced most of the pathologic picture of Sekoke. Potential Usefuness of the Mod
The ability to produce a disease closely resembling human juvenile diabetes mellitus both clinically and histologically by dietarv manipulation offers far better flexibility to the experimenter than do pancreatic Publication sponsored by the Registry of Comparative Pathology of the Armed Forces Institute of b% Public Health Service Grant RR 00301 from the Division of Research Resources. US Department of Health. Education and Welfare. under the auspices of Universities Associated for Research and Education in Pathology. Inc All illustrations are bv Dr. M1otovoshi Yokote of the Freshwater Fisheries Research Laborator^Kino. Tokyo. Japan, and are published by his permission. 805
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