Clin. exp. Immunol. (1991) 84, 83-91
ADONIS
000991049100108V
Antibody penetration of viable human cells. I. Increased penetration of human lymphocytes by anti-RNP IgG J. MA, G. V. CHAPMAN, S.-L. CHEN*, G. MELICKt, R. PENNY & S. N. BREIT Centre for Immunology, St Vincent's Hospital and University of New South Wales, Sydney, NSW, Australia, *Department of Clinical Immunology, Ren Ji Hospital, Shanghai No. 2 Medical University and Shanghai Institute of Immunology, Shanghai, China, and tTissue Typing Laboratory, Australian Red Cross Society of Sydney, Sydney, NSW Australia (Acceptedfor publication 23 October 1990)
SUMMARY Antibody penetration of viable cells and interaction with intracellular antigens may have major consequences for immunopathological processes in connective tissue diseases. We have reported previously that antibody can penetrate viable human lymphocytes. To assess further the role of antinuclear antibodies in this process, peripheral blood lymphocytes (PBMC) were incubated with FITC-conjugated IgG fractions from sera containing anti-RNP (anti-RNP IgG), Ro(SS-A), La(SS-B) and dsDNA antibodies and control sera for 24 h. Using crystal violet to quench cell surface staining, intracellular fluorescence of viable lymphocytes was quantified on the flow cytometer. It was noted that anti-RNP IgG entered 46-4 + 7-2% of lymphocytes which was significantly higher than anti-Ro(SS-A) (29-9+4-1%, P
ADONIS
000991049100108V
Antibody penetration of viable human cells. I. Increased penetration of human lymphocytes by anti-RNP IgG J. MA, G. V. CHAPMAN, S.-L. CHEN*, G. MELICKt, R. PENNY & S. N. BREIT Centre for Immunology, St Vincent's Hospital and University of New South Wales, Sydney, NSW, Australia, *Department of Clinical Immunology, Ren Ji Hospital, Shanghai No. 2 Medical University and Shanghai Institute of Immunology, Shanghai, China, and tTissue Typing Laboratory, Australian Red Cross Society of Sydney, Sydney, NSW Australia (Acceptedfor publication 23 October 1990)
SUMMARY Antibody penetration of viable cells and interaction with intracellular antigens may have major consequences for immunopathological processes in connective tissue diseases. We have reported previously that antibody can penetrate viable human lymphocytes. To assess further the role of antinuclear antibodies in this process, peripheral blood lymphocytes (PBMC) were incubated with FITC-conjugated IgG fractions from sera containing anti-RNP (anti-RNP IgG), Ro(SS-A), La(SS-B) and dsDNA antibodies and control sera for 24 h. Using crystal violet to quench cell surface staining, intracellular fluorescence of viable lymphocytes was quantified on the flow cytometer. It was noted that anti-RNP IgG entered 46-4 + 7-2% of lymphocytes which was significantly higher than anti-Ro(SS-A) (29-9+4-1%, P
