Assembly of the Mammalian Muscle Acetylcholine Receptor in Transfected COS Cells
Yong Gu, John R. Forsayeth, Shahla Verrall, Xiao Mei Yu, and Zach W. Hall
Department ofPhysiology, School ofMedicine, University ofCalifornia, San Francisco, San Francisco, California 94143-0444
Abstract. We have investigated the mechanisms of as-
sembly and transport to the cell surface of the mouse muscle nicotinic acetylcholine receptor (AM) in transiently transfected COS cells. In cells transfected with all four subunit cDNAs, AChR was expressed on the surface with properties resembling those seen in mouse muscle cells (Gu, Y., A . F. Franco, Jr., P D. Gardner, J. B. Lansman, J. R. Forsayeth, and Z. W. Hall. 1990. Neuron . 5:147-157) . When incomplete combinations of AChR subunits were expressed, surface binding of 'III-a-bungarotoxin was not detected except in the case of aOy which expressed
Assembly of the mammalian muscle acetylcholine receptor in transfected COS cells.
We have investigated the mechanisms of assembly and transport to the cell surface of the mouse muscle nicotinic acetylcholine receptor (AChR) in trans...