RESEARCH ARTICLE
Bacteriophage preparation lytic for Shigella significantly reduces Shigella sonnei contamination in various foods Nitzan Soffer*, Joelle Woolston, Manrong Li, Chythanya Das, Alexander Sulakvelidze Intralytix, Inc., Baltimore, Maryland, United States of America * [email protected]
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OPEN ACCESS Citation: Soffer N, Woolston J, Li M, Das C, Sulakvelidze A (2017) Bacteriophage preparation lytic for Shigella significantly reduces Shigella sonnei contamination in various foods. PLoS ONE 12(3): e0175256. https://doi.org/10.1371/journal. pone.0175256 Editor: Mikael Skurnik, University of Helsinki, FINLAND Received: January 16, 2017
Abstract ShigaShield™ is a phage preparation composed of five lytic bacteriophages that specifically target pathogenic Shigella species found in contaminated waters and foods. In this study, we examined the efficacy of various doses (9x105-9x107 PFU/g) of ShigaShield™ in removing experimentally added Shigella on deli meat, smoked salmon, pre-cooked chicken, lettuce, melon and yogurt. The highest dose (2x107 or 9x107 PFU/g) of ShigaShield™ applied to each food type resulted in at least 1 log (90%) reduction of Shigella in all the food types. There was significant (P
Bacteriophage preparation lytic for Shigella significantly reduces Shigella sonnei contamination in various foods Nitzan Soffer*, Joelle Woolston, Manrong Li, Chythanya Das, Alexander Sulakvelidze Intralytix, Inc., Baltimore, Maryland, United States of America * [email protected]
a1111111111 a1111111111 a1111111111 a1111111111 a1111111111
OPEN ACCESS Citation: Soffer N, Woolston J, Li M, Das C, Sulakvelidze A (2017) Bacteriophage preparation lytic for Shigella significantly reduces Shigella sonnei contamination in various foods. PLoS ONE 12(3): e0175256. https://doi.org/10.1371/journal. pone.0175256 Editor: Mikael Skurnik, University of Helsinki, FINLAND Received: January 16, 2017
Abstract ShigaShield™ is a phage preparation composed of five lytic bacteriophages that specifically target pathogenic Shigella species found in contaminated waters and foods. In this study, we examined the efficacy of various doses (9x105-9x107 PFU/g) of ShigaShield™ in removing experimentally added Shigella on deli meat, smoked salmon, pre-cooked chicken, lettuce, melon and yogurt. The highest dose (2x107 or 9x107 PFU/g) of ShigaShield™ applied to each food type resulted in at least 1 log (90%) reduction of Shigella in all the food types. There was significant (P
