Ch inJIn teg r Med2014Feb ;20 (2 ) :123 -129

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OR IG INALART ICLE Bea rB i lePowde r(熊胆粉)InducesApop tos iso f HumanHepa toce l lu la rCa rc inomaCe l lsv ia M i tochond r ion -Dependen tPa thway 1 ,2 3 1 1 ZHAOJ in -yan(赵锦燕) ,CHENZh i -hong( 陈志鸿) ,L IN We i( 林 薇) ,ZHONGX iao -yong( 钟小勇) , 1 1 1 CHENXu -zheng(陈旭征),PENGJun( 彭 军),andHONGZhen feng( 洪振丰)

j e c t i v e:Toe ABSTRACT Ob va lua tethee f fe c to fBea rB i lePowde r( 熊胆粉,BBP )ontheg row thandapop to s i s o f HepG2humanhepa to ce l lu la rca r c inomace l l s ,andin ve s t iga tethepo s s ib le mo le cu la r me chan i sm s med ia t ing e th od s: HepG2ce s: i t san t i can ce ra c t i v i t y .M l l s we ret rea ted w i th0 .4–1 .0 mg /mLo fBBPfo r24 ,48and72h .The v iab i l i tyo f HePG2ce l ls wasde te rm inedby MTTassay . Ce l lu la r mo rpho logy wasobse rvedv iaphase -con t ras t m i c ro s cop y .F luo re s cen ce -a c t i va tedce l lso r t ingana l y s i sw i thAnne x in -V /p rop id ium idod ideand5 ,5 ' ,6 ,6 ' te t ra ch lo ro 1 ,1 ' ,3 ,3 ' te t rae th y l -ben z im ida zo l ca rbo c yan ineiod ide( JC -1 )s ta in ing wa spe r fo rmedtode te rm inece l lapop to s i s andthelo s so fm i to chond r ia l memb ranepo ten t ia l ,re spe c t i ve l y .A c t i va t iono fca spa se -9and-3 wa se va lua tedb y e su l t s:Thet aco lo r ime t r i ca s sa y .R rea tmen tw i th0 .4–1 mg /mLo fBBPfo r24 ,48 ,o r72hre spe c t i ve l yredu cedce l l P