European Journal of Clinical Investigation (1992) 22, 767-771
Can urinary pyridinium crosslinks and urinary oestrogens predict bone mass and rate of bone loss after the menopa use? P. A. MOLE, M. H. WALKINSHAW, S. P. ROBINS* & C. R. PATERSON Department of Biochemical Medicine, Ninewells Hospital and Medical School, Dundee and *Biochemical Sciences Division, Rowett Research Institute, Aberdeen, UK Received 4 December 1991 and in revised form 8 May 1992; accepted 21 May 1992
Abstract. We measured urinary pyridinoline and deoxy-pyridinoline by high performance liquid chromatography, and urinary oestrogens by radioimmunoassay, in 68 healthy postmenopausal women to evaluate these assays for the prediction soon after the menopause of the risk of developing osteoporotic fractures in later life. Change in forearm bone mineral content was assessed by single photon absorptiometry over 4 years. Although there was no significant correlation between the pyridinium crosslinks and urinary oestrogens, we found that up to 58% of the variation in the rate of loss of bone mineral content in women soon after the menopause could be explained by pyridinoline and oestradiol glucuronide assays together with body mass index. Measurement of the urinary pyridinium crosslinks and oestradiol glucuronide may make a significant contribution to a biochemical screening procedure for future osteoporotic fracture. Keywords. Bone density, bone loss, estrogens, hydroxypyridinium crosslinks, menopause, osteoporosis. Introduction
Measurements of the urinary excretion of the hydroxypyridinium crosslinks of collagen, pyridinoline (Pyd) and deoxy-pyridinoline (Dpd), may provide indices of bone resorption [l]. These crosslinks reflect the degradation of mature collagen and not of any intermediate components. Dpd is derived only from bone collagen but Pyd is also derived from collagen in a number of other tissues although not skin. For normal individuals values for Pyd are highly correlated with those for Dpd (r=0.83; P
Can urinary pyridinium crosslinks and urinary oestrogens predict bone mass and rate of bone loss after the menopa use? P. A. MOLE, M. H. WALKINSHAW, S. P. ROBINS* & C. R. PATERSON Department of Biochemical Medicine, Ninewells Hospital and Medical School, Dundee and *Biochemical Sciences Division, Rowett Research Institute, Aberdeen, UK Received 4 December 1991 and in revised form 8 May 1992; accepted 21 May 1992
Abstract. We measured urinary pyridinoline and deoxy-pyridinoline by high performance liquid chromatography, and urinary oestrogens by radioimmunoassay, in 68 healthy postmenopausal women to evaluate these assays for the prediction soon after the menopause of the risk of developing osteoporotic fractures in later life. Change in forearm bone mineral content was assessed by single photon absorptiometry over 4 years. Although there was no significant correlation between the pyridinium crosslinks and urinary oestrogens, we found that up to 58% of the variation in the rate of loss of bone mineral content in women soon after the menopause could be explained by pyridinoline and oestradiol glucuronide assays together with body mass index. Measurement of the urinary pyridinium crosslinks and oestradiol glucuronide may make a significant contribution to a biochemical screening procedure for future osteoporotic fracture. Keywords. Bone density, bone loss, estrogens, hydroxypyridinium crosslinks, menopause, osteoporosis. Introduction
Measurements of the urinary excretion of the hydroxypyridinium crosslinks of collagen, pyridinoline (Pyd) and deoxy-pyridinoline (Dpd), may provide indices of bone resorption [l]. These crosslinks reflect the degradation of mature collagen and not of any intermediate components. Dpd is derived only from bone collagen but Pyd is also derived from collagen in a number of other tissues although not skin. For normal individuals values for Pyd are highly correlated with those for Dpd (r=0.83; P
