THROMBOSIS
RESEARCH 65; 663-665,1992
0049-3848/92 $5.00+ .OOPrinted in the USA. Copyright (c) 1992 Pergamon Press Ltd. All rights reserved
i-1. anowska’ 2
R.
PO1
b.
,Allg~~si.).n i ak
I)epnrt.ment. Tnt.ernal
i.i1dley
Wi 1czyfi.ska’ , Cierniewski
I”.
SIawiiiski
2
i
.T. H.
Goch2,
, C. S.
of’ Ric3ptrysics. Pledical Scl1001 II i sease i HiI i t.ar-y Hedi cal
st.l-wt.,
Polallrl.
(Received 27.51991; accepted in revised form 18.11 .1991 by Editor S. Lopaciuk) (Received by Executive Editorial Off ice 7.1 .1992)
~~rtxriography is hasi c part, i CII 1al- 1 y i il Lhe esamj na t. i on, t.r-ea t.ment. for 1 i nit:, of qIral.ifyirlg pat.ient.s t.0 si~rgir:aJ iscilaemia. Strch an invnsi\.e \.asc111 ar- esami rla1.i on may resell t. in t.he damage of vascular euclotheliitm and t.hits j t. ma y .inf’l llence blood plasma and plat.elet. IlaemosLasjs. III report. we this rlesr:rihe plasma concent.rat.ion of some protzins released f'rorn l-11ood plat.el ets p 1at..el et. az;;,;; ~~~~-t.~~r~onibogl.ot~~.~l in, ,,” .i a 1-1cl endotlielia 1 cells C t.issi.re plasmi r~ogen and fihronect..in) afker a single inkaart.eria1 i n ject.j on of’ an ionic col1t.rasl. niediIun.
t.errrls
SIIR,.JECTS
-AND HFTHODS IL
performed on 26 III~II aged f’r-oirr 3i 1-m 73 years k==par-t.ment. of’ Per i pl-I~I-i3 1 Vasr:lI 1al- Di seases I.odz Flecii.c:w 1 sCt1001, Aia+1t,ern~rlert,th~l pat.j eni. i sr:llemir: syInpt,~ls j n Irgs rllle t,o peripllera 1 , : . occ:Illsj \'F? rl'i-ease and were i.11 t.he second or t.hi.rd st;age of t.i,e di sease ac:corri i IIQ t.0 t.he Pont.aine c:la.~sil’icai.ion. Arigi ography was rioIIe 1 (_I i ,*gei11sing Se (.ile t.er:f-ir1-i c111e (1). Cal.het.erwas .i r1t.rorl~lc:ecl tindera I or:3 1 aiiesi.lles.i t.liI-C>llgll t.lle f'ernorfll. ni--t.ery t.0 t.lle aor1.a at. the 1e\:el ol‘ lJ3e rerral art.erie.s. Then 00 1111 of 7% III-opal i11 sol.Ilt.j.or-1 (‘Z. F. Poll’s, St.ar-ogarrl.1) arr iori i.c contxast. med i iuri , was trncler in ject.ed short. geriera 1 anest,he.si a tisin,p an a1rLomat.i c under 6l.r i nge pressirre of 3 atmospheres. Sl.llcli
arIm i i.tzd
were
es
t.0
the
??
663
PLATELETS AND ARTERIOGRAPHY
664
Vol. 65. Nos. 415
t.aken from t.he a0rt.a arid t.he cuhi tal. vej n ii1 ood sampl.es were immediat.ely before t.lre in ject.ion of c:ont.ras(. mediilm and 3 t.0 5 mi.~~ af t.er t.he in jec:t.i 011. pJ.at.el et. fact.or4> Pl at.el.et, proteins ~.(3-t.l-~ronrho~J.ol~~~l j II and blood f ibronect.in were det.ermi ned i II plasma by and c2 , 3) . described previoiisl y radi oinmutnoassy a .s Tj sslle wa.s measured in 111ood plasma of pat..j.ents pl asm.inogan ac:t.i.vat,orf i,hr-ynoJ yt,ic assay Irsi ng pal yst.yrene best Ily a so1.i cl phase l..uhe.s coat.ed with radiaiodinated fillman f ihrj n (4). Data were arlalysed by Fisher’s F t,est. for variance analysis and compared Ilsing t.he tinpaired St.lltlent. t.-t..est,.
RESIILTS As it. i.s seen .i II Tat31 e 1 , Lhe in ject..iorl of contzast. macli.l~m catises a limj.t.ed act..i \:at.i.on of lrlood plat..el et.s docllment.ed kry an i ncrease i 1-1(2-t.l~roml~ogl ohrrl i n level i II 111ootl samples i.aken from \:eirl . There was al.so a significant. increase in t.issue plasminogen act.i.vat.orac:t.ivif.y in art.er-j al I31ood pi asma af t.er art..er-i ography Cp < 0. WJ> .
Tahle 1 PI asma Conc:erit.rat.i on of prot.ei 11,s r-e1 eased by pl at.el ets and endot.hel.ial cells af’t.ar arteri ogr-aptly. Amo1rrrt.s of Q-t.hronih0g.i obul in and PI at.el et. Fact,or S are expressed in ng4n1, fi hronect,j.n i II plasminogen $!g/ml , and kisslie act.i vat..or in pmoles of hydrol ysed ’ Z51-fihrin/per ml of plasma in 1 min. ARTERIAL Protein
RLOOD
Def’or~
p-TG
SE..8-+17.9 + 16. J-+.
F’FJ-
VENOUS
Af t.er 38. 9
s+13.4 +
J--n
+-l-s6 +-z35
302
L-PA
156
208-73
+68
$5
Aft.er
Ref ore
I’
13.ti-6.1
BLOOD
50.2
+I
P 23.6
p
PTAO: %. E. ~ MIJRDOCK, D. f;. . MESSMORE. H. I,. ; GTARDINA, J . ,r : SCANLOM, P . ..T. ; Risk of t.h romboerriho 1 i sm cluri ng i c and Cl i agrlost. irrt.ervent.iotlal carrli ac procerliu-es wi t.h ikoni oni c: coirt.ra.st. karl i 13Logon -L 1 74 453-4.57, rued i a .
19Yt3.
STORMORKEN, H. _ SKALPE, 1,. 0. , TESTARD. various cont..rast. metli a nn coaglrl at.i 011. plat,el et.. flinct.ion: an i n vi t.r0 ilIlCl Tnvest.. :34n-354. ) 1 RFlf;. RadioI. 31 LL Plat.ele(. endot.hel ium in 24, 1!390.
GRABOWSKT , E. C. vasc:lrlar 25, 123-l
adhesi
flowi
M. C. Ef fee t.?;; of f’il_lrinoJ ysi 5, and j. n v i vo st.lldy.
on/agregat..ion
II~ blood.
f.0 Invest..
irr jiir-ccl Rarl i 03..
GR4BOWSGT, E.G. : 4 hemat.ologj.st.‘s view of r:ont.rast. media, cl.ot.+t.ing in angi ogr-aphy duri ng syringes arlcl t.hromlx3s.i s coronary angiography. Am. ,J._Cardio1.. 6fi. 23-25. 1990.
RESEARCH 65; 663-665,1992
0049-3848/92 $5.00+ .OOPrinted in the USA. Copyright (c) 1992 Pergamon Press Ltd. All rights reserved
i-1. anowska’ 2
R.
PO1
b.
,Allg~~si.).n i ak
I)epnrt.ment. Tnt.ernal
i.i1dley
Wi 1czyfi.ska’ , Cierniewski
I”.
SIawiiiski
2
i
.T. H.
Goch2,
, C. S.
of’ Ric3ptrysics. Pledical Scl1001 II i sease i HiI i t.ar-y Hedi cal
st.l-wt.,
Polallrl.
(Received 27.51991; accepted in revised form 18.11 .1991 by Editor S. Lopaciuk) (Received by Executive Editorial Off ice 7.1 .1992)
~~rtxriography is hasi c part, i CII 1al- 1 y i il Lhe esamj na t. i on, t.r-ea t.ment. for 1 i nit:, of qIral.ifyirlg pat.ient.s t.0 si~rgir:aJ iscilaemia. Strch an invnsi\.e \.asc111 ar- esami rla1.i on may resell t. in t.he damage of vascular euclotheliitm and t.hits j t. ma y .inf’l llence blood plasma and plat.elet. IlaemosLasjs. III report. we this rlesr:rihe plasma concent.rat.ion of some protzins released f'rorn l-11ood plat.el ets p 1at..el et. az;;,;; ~~~~-t.~~r~onibogl.ot~~.~l in, ,,” .i a 1-1cl endotlielia 1 cells C t.issi.re plasmi r~ogen and fihronect..in) afker a single inkaart.eria1 i n ject.j on of’ an ionic col1t.rasl. niediIun.
t.errrls
SIIR,.JECTS
-AND HFTHODS IL
performed on 26 III~II aged f’r-oirr 3i 1-m 73 years k==par-t.ment. of’ Per i pl-I~I-i3 1 Vasr:lI 1al- Di seases I.odz Flecii.c:w 1 sCt1001, Aia+1t,ern~rlert,th~l pat.j eni. i sr:llemir: syInpt,~ls j n Irgs rllle t,o peripllera 1 , : . occ:Illsj \'F? rl'i-ease and were i.11 t.he second or t.hi.rd st;age of t.i,e di sease ac:corri i IIQ t.0 t.he Pont.aine c:la.~sil’icai.ion. Arigi ography was rioIIe 1 (_I i ,*gei11sing Se (.ile t.er:f-ir1-i c111e (1). Cal.het.erwas .i r1t.rorl~lc:ecl tindera I or:3 1 aiiesi.lles.i t.liI-C>llgll t.lle f'ernorfll. ni--t.ery t.0 t.lle aor1.a at. the 1e\:el ol‘ lJ3e rerral art.erie.s. Then 00 1111 of 7% III-opal i11 sol.Ilt.j.or-1 (‘Z. F. Poll’s, St.ar-ogarrl.1) arr iori i.c contxast. med i iuri , was trncler in ject.ed short. geriera 1 anest,he.si a tisin,p an a1rLomat.i c under 6l.r i nge pressirre of 3 atmospheres. Sl.llcli
arIm i i.tzd
were
es
t.0
the
??
663
PLATELETS AND ARTERIOGRAPHY
664
Vol. 65. Nos. 415
t.aken from t.he a0rt.a arid t.he cuhi tal. vej n ii1 ood sampl.es were immediat.ely before t.lre in ject.ion of c:ont.ras(. mediilm and 3 t.0 5 mi.~~ af t.er t.he in jec:t.i 011. pJ.at.el et. fact.or4> Pl at.el.et, proteins ~.(3-t.l-~ronrho~J.ol~~~l j II and blood f ibronect.in were det.ermi ned i II plasma by and c2 , 3) . described previoiisl y radi oinmutnoassy a .s Tj sslle wa.s measured in 111ood plasma of pat..j.ents pl asm.inogan ac:t.i.vat,orf i,hr-ynoJ yt,ic assay Irsi ng pal yst.yrene best Ily a so1.i cl phase l..uhe.s coat.ed with radiaiodinated fillman f ihrj n (4). Data were arlalysed by Fisher’s F t,est. for variance analysis and compared Ilsing t.he tinpaired St.lltlent. t.-t..est,.
RESIILTS As it. i.s seen .i II Tat31 e 1 , Lhe in ject..iorl of contzast. macli.l~m catises a limj.t.ed act..i \:at.i.on of lrlood plat..el et.s docllment.ed kry an i ncrease i 1-1(2-t.l~roml~ogl ohrrl i n level i II 111ootl samples i.aken from \:eirl . There was al.so a significant. increase in t.issue plasminogen act.i.vat.orac:t.ivif.y in art.er-j al I31ood pi asma af t.er art..er-i ography Cp < 0. WJ> .
Tahle 1 PI asma Conc:erit.rat.i on of prot.ei 11,s r-e1 eased by pl at.el ets and endot.hel.ial cells af’t.ar arteri ogr-aptly. Amo1rrrt.s of Q-t.hronih0g.i obul in and PI at.el et. Fact,or S are expressed in ng4n1, fi hronect,j.n i II plasminogen $!g/ml , and kisslie act.i vat..or in pmoles of hydrol ysed ’ Z51-fihrin/per ml of plasma in 1 min. ARTERIAL Protein
RLOOD
Def’or~
p-TG
SE..8-+17.9 + 16. J-+.
F’FJ-
VENOUS
Af t.er 38. 9
s+13.4 +
J--n
+-l-s6 +-z35
302
L-PA
156
208-73
+68
$5
Aft.er
Ref ore
I’
13.ti-6.1
BLOOD
50.2
+I
P 23.6
p
PTAO: %. E. ~ MIJRDOCK, D. f;. . MESSMORE. H. I,. ; GTARDINA, J . ,r : SCANLOM, P . ..T. ; Risk of t.h romboerriho 1 i sm cluri ng i c and Cl i agrlost. irrt.ervent.iotlal carrli ac procerliu-es wi t.h ikoni oni c: coirt.ra.st. karl i 13Logon -L 1 74 453-4.57, rued i a .
19Yt3.
STORMORKEN, H. _ SKALPE, 1,. 0. , TESTARD. various cont..rast. metli a nn coaglrl at.i 011. plat,el et.. flinct.ion: an i n vi t.r0 ilIlCl Tnvest.. :34n-354. ) 1 RFlf;. RadioI. 31 LL Plat.ele(. endot.hel ium in 24, 1!390.
GRABOWSKT , E. C. vasc:lrlar 25, 123-l
adhesi
flowi
M. C. Ef fee t.?;; of f’il_lrinoJ ysi 5, and j. n v i vo st.lldy.
on/agregat..ion
II~ blood.
f.0 Invest..
irr jiir-ccl Rarl i 03..
GR4BOWSGT, E.G. : 4 hemat.ologj.st.‘s view of r:ont.rast. media, cl.ot.+t.ing in angi ogr-aphy duri ng syringes arlcl t.hromlx3s.i s coronary angiography. Am. ,J._Cardio1.. 6fi. 23-25. 1990.
