CHARACTERIZATION OF BOVINE FOLLICULAR OOCYTES AND THEIR ABILITY TO MATURE I N V I T R O 1 L. Leibfried and N. L. First

University o f Wisconsin 2, Madison 53706

SUMMARY

Three experiments were conducted to characterize the follicular oocytes of bovine ovaries and to assess the oocyte's ability to mature in vitro. Experiment 1 was designed to describe the oocytes from surface follicles of early luteal, luteal or follicular stage ovaries. Oocytes were also categorized on the basis of follicle size (1 to 3 or > 3 mm). Three classification schemes with numerical values assigned were developed to characterize investments of the oocyte, state of the ooplasm and stage of nuclear development. Characteristics typical of oocytes from nonatretic, vesicular follicles were given values of one. Characteristics with higher values were considered to be signs of degeneration. Oocytes from nine co~s per stage were evaluated. A higher proportion of nondegenerate investments was found in follicles 1 to 3 mm in size (P

Characterization of bovine follicular oocytes and their ability to mature in vitro.

CHARACTERIZATION OF BOVINE FOLLICULAR OOCYTES AND THEIR ABILITY TO MATURE I N V I T R O 1 L. Leibfried and N. L. First University o f Wisconsin 2, Ma...
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