6424 Nucleic Acids Research, Vol. 18, No. 21
Cloning and nucleotide sequence of mink growth hormone
a
cDNA encoding the
Kazuaki Shoji*, Eiji Ohara, Masanori Watahiki and Yuko Yoneda Enzymological Research Laboratory, Nippon Gene Co. Ltd, 1-29, Tonya-machi, Toyama 930, Japan Submitted October 1, 1990
EMBL accession no. X56120
We report the nucleotide sequence of a cDNA clone encoding a complete amino acid sequence of mink growth hormone (mGH). The cDNA clone was isolated from a pUC19 cDNA library derived from mink pituitary poly(A)+ RNA using a porcine GH cDNA as a probe. The 783 base pairs (bp) cDNA clone has an open reading frame of 648 bp and the deduced amino acid sequence is 216 residues with a 24,469 daltons. Four cysteine residues (boxed) which may participate in the construction of structural features present in mGH at the same positions as those for other vertebrate GHs (1). The mGH shares 96%, 90%, 88%, 87% and 66% amino acid homology with the known mammalian sequences from porcine (2), mouse (3) or rat (4), bovine (5), goat (6) and human (7), respectively. The putative signal sequence is underlined and the polyadenylation signal is double underlined.
REFERENCES 1. Watahiki,M., Yamamoto,M., Yamakawa,M., Tanaka,M. and Nakashima,K. (1989) J. Biol. Chem. 264, 312-316. 2. Vize,P.D. and Wells,R.E. (1987) Gene 55, 339-344. 3. Linzer,D.I.H. and Talamantes,F. (1985) J. Biol. Chem. 260, 9574-9579. 4. Page,G.S., Smith,S. and Goodman,H.M. (1981) Nucl. Acids Res. 9, 2087-2104. 5. Woychik,R.P., Camper,S.A., Lyons,R.H., Horowitz,S., Goodwin,E.C. and Rottman,F.M. (1982) Nucl. Acids Res. 10, 7197-7210. 6. Yamamoto,Y., Oyabayashi,K., Okuno,M., Yato,M., Kioka,N., Manabe,E., Hashi,H., Sakai,H., Komano,T., Utsumi,K. and Iritani,A. (1988) FEBS Leu. 228, 301-304. 7. DeNoto,F.M., Moore,D.D. and Goodman,H.M. (1981) Nucl. Acids Res. 9, 3719-3730.
CTCAGGGTCCTGTGGACAGCTCACCTGGCTGCA
-1
ATGGCTGCTGGTCCTCGGAACTCCATGCTCCTGGTGTTCGCCCTGCTCAGCCTGCCCTGGCCTCAGGAGGTG
72 24
M
A
A
G
P
R
N
S
M
L
L
V
F
A
L
L
S
L
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W
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GGCGCCTTCCCGGCCATGCCCTTGTCCAGCCTGTTTGCCAACGCCGTGCTCCGGGCCCAGCACCTGCACCAG G
A
F
P
A
M
P
L
S
S
F
A
N
A
V
L
R
A
Q
H
L
H
Q
144 48
CTGGCTGCCGACACCTACAAAGACTTTGAGCGGGCGTACATCCCCGAGGGCCAGAGGTACTCCATCCAGAAC L
216 72
GCGCAGGCTGCCTTCTGCTTCTCGGAGACCATCCCGGCGCCCACCGGCAAGGACGAGGCCCAGCAGAGATCC A T
I
288 96
IJIAILAI 1 UAjtijlU-*U -TTTjIT I; D M E L L R F S L L
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360 120
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CATICAGTiTCGTGMGCTGGGGCCCGTGCAGTTCCTCAGCAGG I
Q
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W
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P
V
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L
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GTCTTCACCAACAGCCTGGTGTTCGGCACCTCGGACCGAGTCTACGAGAAGCTGAAGGACCTGGAGGAAGGC V F T
432 144
ATCCAAGCGCTGATGCGGGAGCTGGAAGACGGCAGCCCCCGGGCCGGGCCGATCCTGAAGCAAACCTACGAC I Q A L M R E
504 168
AAGTTTGACACAAACCTGCGCAGCGACGACGCGCTGCTCAAGAACTATGGGCTGCTCTCCTGCTTCAAGAAG K F D
576 192
GACCTGCACAAGGCCGAGACGTATCTGCGGGTCATGAAGTGTCGCCGCTTCGTGGAAAGCAGCTGTGCCTTC D
648 216
TAGTTCGCGGGCTTTCCTTTCAGCCCGGCCCCACAGCCTCCCCTGACCCTGGAAAGGGCCTCTCCCGGTGTC
720
N
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Cloning and nucleotide sequence of mink growth hormone
a
cDNA encoding the
Kazuaki Shoji*, Eiji Ohara, Masanori Watahiki and Yuko Yoneda Enzymological Research Laboratory, Nippon Gene Co. Ltd, 1-29, Tonya-machi, Toyama 930, Japan Submitted October 1, 1990
EMBL accession no. X56120
We report the nucleotide sequence of a cDNA clone encoding a complete amino acid sequence of mink growth hormone (mGH). The cDNA clone was isolated from a pUC19 cDNA library derived from mink pituitary poly(A)+ RNA using a porcine GH cDNA as a probe. The 783 base pairs (bp) cDNA clone has an open reading frame of 648 bp and the deduced amino acid sequence is 216 residues with a 24,469 daltons. Four cysteine residues (boxed) which may participate in the construction of structural features present in mGH at the same positions as those for other vertebrate GHs (1). The mGH shares 96%, 90%, 88%, 87% and 66% amino acid homology with the known mammalian sequences from porcine (2), mouse (3) or rat (4), bovine (5), goat (6) and human (7), respectively. The putative signal sequence is underlined and the polyadenylation signal is double underlined.
REFERENCES 1. Watahiki,M., Yamamoto,M., Yamakawa,M., Tanaka,M. and Nakashima,K. (1989) J. Biol. Chem. 264, 312-316. 2. Vize,P.D. and Wells,R.E. (1987) Gene 55, 339-344. 3. Linzer,D.I.H. and Talamantes,F. (1985) J. Biol. Chem. 260, 9574-9579. 4. Page,G.S., Smith,S. and Goodman,H.M. (1981) Nucl. Acids Res. 9, 2087-2104. 5. Woychik,R.P., Camper,S.A., Lyons,R.H., Horowitz,S., Goodwin,E.C. and Rottman,F.M. (1982) Nucl. Acids Res. 10, 7197-7210. 6. Yamamoto,Y., Oyabayashi,K., Okuno,M., Yato,M., Kioka,N., Manabe,E., Hashi,H., Sakai,H., Komano,T., Utsumi,K. and Iritani,A. (1988) FEBS Leu. 228, 301-304. 7. DeNoto,F.M., Moore,D.D. and Goodman,H.M. (1981) Nucl. Acids Res. 9, 3719-3730.
CTCAGGGTCCTGTGGACAGCTCACCTGGCTGCA
-1
ATGGCTGCTGGTCCTCGGAACTCCATGCTCCTGGTGTTCGCCCTGCTCAGCCTGCCCTGGCCTCAGGAGGTG
72 24
M
A
A
G
P
R
N
S
M
L
L
V
F
A
L
L
S
L
P
W
P
Q
E
V
GGCGCCTTCCCGGCCATGCCCTTGTCCAGCCTGTTTGCCAACGCCGTGCTCCGGGCCCAGCACCTGCACCAG G
A
F
P
A
M
P
L
S
S
F
A
N
A
V
L
R
A
Q
H
L
H
Q
144 48
CTGGCTGCCGACACCTACAAAGACTTTGAGCGGGCGTACATCCCCGAGGGCCAGAGGTACTCCATCCAGAAC L
216 72
GCGCAGGCTGCCTTCTGCTTCTCGGAGACCATCCCGGCGCCCACCGGCAAGGACGAGGCCCAGCAGAGATCC A T
I
288 96
IJIAILAI 1 UAjtijlU-*U -TTTjIT I; D M E L L R F S L L
L
360 120
A
Q
A A
D
A
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F
Y
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CATICAGTiTCGTGMGCTGGGGCCCGTGCAGTTCCTCAGCAGG I
Q
S
W
L
G
P
V
Q
F
L
S
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GTCTTCACCAACAGCCTGGTGTTCGGCACCTCGGACCGAGTCTACGAGAAGCTGAAGGACCTGGAGGAAGGC V F T
432 144
ATCCAAGCGCTGATGCGGGAGCTGGAAGACGGCAGCCCCCGGGCCGGGCCGATCCTGAAGCAAACCTACGAC I Q A L M R E
504 168
AAGTTTGACACAAACCTGCGCAGCGACGACGCGCTGCTCAAGAACTATGGGCTGCTCTCCTGCTTCAAGAAG K F D
576 192
GACCTGCACAAGGCCGAGACGTATCTGCGGGTCATGAAGTGTCGCCGCTTCGTGGAAAGCAGCTGTGCCTTC D
648 216
TAGTTCGCGGGCTTTCCTTTCAGCCCGGCCCCACAGCCTCCCCTGACCCTGGAAAGGGCCTCTCCCGGTGTC
720
N
S
L
V
F
L
T
L
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CTCTGTGCTTTCCTAATAAAATGAAATTGC
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To whom correspondence should be addressed
S
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