YEAST
0 0
00
VOL.
7: 993-995 (1 991)
0 0 0
x %YeastSequencing Reports
Cloning of a Saccharomyces cerevisiae Gene Encoding a Protein Homologous to Allantoicase of Neurospora crassa FANG-JEN S. LEE* AND JOEL MOSS Laboratory of Cellular Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, M D 20892, U . S . A .
Received and accepted 13 August 1991 KEY WORDS - Saccharomyces cerevisiae; chromosome 111; allantoicase; purine catabolism.
We have cloned a 4.5 kb genomic DNA from Saccharomyces cerevisiae h DASH library (Stratagene) which contains two open reading frames. The DNA has been sequenced (both strands) using Sequenase (US Biochemical) and appropriate overlapping sequence runs. The nucleotide sequence of one open reading frame, including flanking regions, and potential TATA box (underlined), is shown in Figure 1. It encodes a 343 amino acid protein product that is 50% identical and 83% homologous to allantoicase (EC 3.5.3.4) of Neurospora crassa (Figure 2 ) (Lee et al., 1990). The putative yeast allantoicase gene (ALCI) is located on chromosome 111 (data not shown), and inferred from its neighbouring sequences, is 900 bp downstream of the other open reading frame, the DAL7 gene (Figure 3) (Yo0 and Cooper, 1989). ALCI and DAL7 are transcribed in opposite directions. Both proteins are involved in purine catabolism. The DALS gene, also involved in purine catabolism, and DAL7 contain six and five copies, respectively, of the UAS pentanucleotide 5’-GATAA-3‘ or its reverse complement 5”-TTATC-3” (Yo0 and Cooper, 1989). The same
0749-503)
0 0
00
VOL.
7: 993-995 (1 991)
0 0 0
x %YeastSequencing Reports
Cloning of a Saccharomyces cerevisiae Gene Encoding a Protein Homologous to Allantoicase of Neurospora crassa FANG-JEN S. LEE* AND JOEL MOSS Laboratory of Cellular Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, M D 20892, U . S . A .
Received and accepted 13 August 1991 KEY WORDS - Saccharomyces cerevisiae; chromosome 111; allantoicase; purine catabolism.
We have cloned a 4.5 kb genomic DNA from Saccharomyces cerevisiae h DASH library (Stratagene) which contains two open reading frames. The DNA has been sequenced (both strands) using Sequenase (US Biochemical) and appropriate overlapping sequence runs. The nucleotide sequence of one open reading frame, including flanking regions, and potential TATA box (underlined), is shown in Figure 1. It encodes a 343 amino acid protein product that is 50% identical and 83% homologous to allantoicase (EC 3.5.3.4) of Neurospora crassa (Figure 2 ) (Lee et al., 1990). The putative yeast allantoicase gene (ALCI) is located on chromosome 111 (data not shown), and inferred from its neighbouring sequences, is 900 bp downstream of the other open reading frame, the DAL7 gene (Figure 3) (Yo0 and Cooper, 1989). ALCI and DAL7 are transcribed in opposite directions. Both proteins are involved in purine catabolism. The DALS gene, also involved in purine catabolism, and DAL7 contain six and five copies, respectively, of the UAS pentanucleotide 5’-GATAA-3‘ or its reverse complement 5”-TTATC-3” (Yo0 and Cooper, 1989). The same
0749-503)
