Gen Thorac Cardiovasc Surg (2014) 62:202–203 DOI 10.1007/s11748-013-0369-3
RAPID COMMUNICATION
Collagen fleece failed to prevent intrapleural adhesions in a canine model: failure of murine models to translate into a large animal model Masatsugu Hamaji • Fumitsugu Kojima Tatsuo Nakamura
•
Received: 4 December 2013 / Accepted: 20 December 2013 / Published online: 9 January 2014 Ó The Japanese Association for Thoracic Surgery 2014
Abstract Intrapleural and anti-adhesive materials were investigated in murine models, but not in a large animal model before clinical application. Our results failed to show any anti-adhesion effect of collagen fleece and suggested the thoracic physiology in murine models would not translate into a large animal model.
Keywords
Thoracotomy Reoperation
Although re-operative thoracic surgery is not uncommon, no intrapleural anti-adhesive materials were applied to clinical practice. The anti-adhesive properties of biode-
Fig. 1 Representative thoracoscopic photos in the left intrapleural space. No collagen fleece was placed on the anterior incision (a); collagen fleece placed on the posterior incision (b). Moderate intrapleural adhesions noted 6 months afterwards on both the control side (c, arrow) and the collagen fleece side (d, arrow)
M. Hamaji (&) F. Kojima T. Nakamura Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan e-mail: [email protected]
123
gradable materials have been only described in murine thoracotomy models [1–3]. A 3-cm anterior mini-thoracotomy and another 3-cm posterior incision were made on 5 adult Beagle dogs. The
Gen Thorac Cardiovasc Surg (2014) 62:202–203
collagen fleece, TachoCombÒ (CSL Behring, King of Prussia, PA, USA) was prepared. No collagen fleece was placed on an anterior incision, while the collagen fleece was placed on the posterior incision (Fig. 1a, b), via OlympusÒ video thoracoscope. Six months later, moderate intrapleural adhesions developed on both incisions (Fig. 1c, d). Our negative results would not completely deny the anti-adhesive effects of collagen fleece, but suggested that thoracic physiology of murine models do not always translate into that of larger animal models. Conflict of interest interest.
203
References 1. Komatsu K, Fujii A, Higami T. Haemostatic fleece (TachoComb) to prevent intrapleural adhesions after thoracotomy: a rat model. Thorac Cardiovasc Surg. 2007;55(6):385–90. 2. Izumi Y, Takahashi Y, Kohno M, Nomori H. Cross-linked poly (gamma-glutamic acid) attenuates pleural and chest wall adhesions in a mouse thoracotomy model. Eur Surg Res. 2012;48(2):93–8. 3. Tanaka A, Abe T, Matsuura A. Prevention of postoperative intrapleural adhesion of the thoracotomy incision by a bioresorbable membrane in the rat adhesion model. Ann Thorac Cardiovasc Surg. 2000;6(3):151–60.
All the authors have declared no competing
123
RAPID COMMUNICATION
Collagen fleece failed to prevent intrapleural adhesions in a canine model: failure of murine models to translate into a large animal model Masatsugu Hamaji • Fumitsugu Kojima Tatsuo Nakamura
•
Received: 4 December 2013 / Accepted: 20 December 2013 / Published online: 9 January 2014 Ó The Japanese Association for Thoracic Surgery 2014
Abstract Intrapleural and anti-adhesive materials were investigated in murine models, but not in a large animal model before clinical application. Our results failed to show any anti-adhesion effect of collagen fleece and suggested the thoracic physiology in murine models would not translate into a large animal model.
Keywords
Thoracotomy Reoperation
Although re-operative thoracic surgery is not uncommon, no intrapleural anti-adhesive materials were applied to clinical practice. The anti-adhesive properties of biode-
Fig. 1 Representative thoracoscopic photos in the left intrapleural space. No collagen fleece was placed on the anterior incision (a); collagen fleece placed on the posterior incision (b). Moderate intrapleural adhesions noted 6 months afterwards on both the control side (c, arrow) and the collagen fleece side (d, arrow)
M. Hamaji (&) F. Kojima T. Nakamura Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan e-mail: [email protected]
123
gradable materials have been only described in murine thoracotomy models [1–3]. A 3-cm anterior mini-thoracotomy and another 3-cm posterior incision were made on 5 adult Beagle dogs. The
Gen Thorac Cardiovasc Surg (2014) 62:202–203
collagen fleece, TachoCombÒ (CSL Behring, King of Prussia, PA, USA) was prepared. No collagen fleece was placed on an anterior incision, while the collagen fleece was placed on the posterior incision (Fig. 1a, b), via OlympusÒ video thoracoscope. Six months later, moderate intrapleural adhesions developed on both incisions (Fig. 1c, d). Our negative results would not completely deny the anti-adhesive effects of collagen fleece, but suggested that thoracic physiology of murine models do not always translate into that of larger animal models. Conflict of interest interest.
203
References 1. Komatsu K, Fujii A, Higami T. Haemostatic fleece (TachoComb) to prevent intrapleural adhesions after thoracotomy: a rat model. Thorac Cardiovasc Surg. 2007;55(6):385–90. 2. Izumi Y, Takahashi Y, Kohno M, Nomori H. Cross-linked poly (gamma-glutamic acid) attenuates pleural and chest wall adhesions in a mouse thoracotomy model. Eur Surg Res. 2012;48(2):93–8. 3. Tanaka A, Abe T, Matsuura A. Prevention of postoperative intrapleural adhesion of the thoracotomy incision by a bioresorbable membrane in the rat adhesion model. Ann Thorac Cardiovasc Surg. 2000;6(3):151–60.
All the authors have declared no competing
123
