Anal Bioanal Chem (2014) 406:7275–7286 DOI 10.1007/s00216-014-8124-x
RESEARCH PAPER
Comparative evaluation of extraction methods for simultaneous mass-spectrometric analysis of complex lipids and primary metabolites from human blood plasma Do Yup Lee & Tobias Kind & Young-Ran Yoon & Oliver Fiehn & Kwang-Hyeon Liu
Received: 18 July 2014 / Revised: 17 August 2014 / Accepted: 19 August 2014 / Published online: 30 August 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract Metabolomic results on human blood plasma largely depend on the sample preparation protocols employed for protein precipitation and metabolite extraction. Five different extraction methods were examined, which can be grouped into two categories, liquid-liquid extraction and protein precipitation methods, including long-standing protocols such as the Folch extraction and Bligh-Dyer extraction in comparison to modern methods such as the Matyash protocol and two global metabolite extraction methods. Extracts were subjected to analysis of blood plasma lipids and primary metabolites by using chip-based direct infusion nanoelectrospray tandem mass spectrometry and gas chromatography coupled to timeof-flight mass spectrometry, respectively. Optimal extraction schemes were evaluated based on the number of identified metabolites, extraction efficiency, compound diversity,
Electronic supplementary material The online version of this article (doi:10.1007/s00216-014-8124-x) contains supplementary material, which is available to authorized users. D. Y. Lee The Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, South Korea D. Y. Lee : T. Kind : O. Fiehn (*) : K.
RESEARCH PAPER
Comparative evaluation of extraction methods for simultaneous mass-spectrometric analysis of complex lipids and primary metabolites from human blood plasma Do Yup Lee & Tobias Kind & Young-Ran Yoon & Oliver Fiehn & Kwang-Hyeon Liu
Received: 18 July 2014 / Revised: 17 August 2014 / Accepted: 19 August 2014 / Published online: 30 August 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract Metabolomic results on human blood plasma largely depend on the sample preparation protocols employed for protein precipitation and metabolite extraction. Five different extraction methods were examined, which can be grouped into two categories, liquid-liquid extraction and protein precipitation methods, including long-standing protocols such as the Folch extraction and Bligh-Dyer extraction in comparison to modern methods such as the Matyash protocol and two global metabolite extraction methods. Extracts were subjected to analysis of blood plasma lipids and primary metabolites by using chip-based direct infusion nanoelectrospray tandem mass spectrometry and gas chromatography coupled to timeof-flight mass spectrometry, respectively. Optimal extraction schemes were evaluated based on the number of identified metabolites, extraction efficiency, compound diversity,
Electronic supplementary material The online version of this article (doi:10.1007/s00216-014-8124-x) contains supplementary material, which is available to authorized users. D. Y. Lee The Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, South Korea D. Y. Lee : T. Kind : O. Fiehn (*) : K.
