Biotherapy 2: 21-32, 1990. © 1990 Kluwer Academic Publishers. Printed in the Netherlands.

Comparison between crude Interleukin-2 and recombinant Interleukin-2 in maintaining killing activity of cultured lymphocytes Masaki Nakanishi, Norimichi Kan, Takashi Okino, Kohei Satoh, Keiichi Mise, Yasufumi Teramura, Seiji Yamasaki, Kazuhisa Ohgaki & Takayoshi Tobe

Laboratory of Oncologic Surgery, First Department of Surgery, School of Medicine, Kyoto University, Kyoto, Japan Received 25 May 1989; accepted 21 June 1989

Key words: crude Interleukin-2, recombinant Interleukin-2, killing activity, IL-2 receptor expression Abstract

Peripheral blood lymphocytes, regional lymph node lymphocytes or malignant effusion lymphocytes from cancer patients were incubated with crude IL-2 (cIL-2) for 13 days. These effectors, which frequently expressed IL-2 receptor (IL-2R), proliferated well and possessed augmented killing activity against fresh autologous tumor cells and K562. However, when recombinant IL-2 (rIL-2) was added for the last 4 days of culture instead of cIL-2, IL-2R expression and killing activity against fresh autologous tumor cells decreased significantly (P < 0.05). Phenotypic analysis indicated that cIL-2 significantly promoted the expansion of the cytotoxic population (CD8+.llb-)(P

Comparison between crude interleukin-2 and recombinant interleukin-2 in maintaining killing activity of cultured lymphocytes.

Peripheral blood lymphocytes, regional lymph node lymphocytes or malignant effusion lymphocytes from cancer patients were incubated with crude IL-2 (c...
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