THROMBOSIS RESEARCH 61; 253-259,199l 0049-3848/91 $3.00 + .OO Printed in the USA. Copyright (c) 1991 Pergamon Press pk. All rights reserved.
COMPARISON FIBRINOPEPTIDE
OF
THROMBIN-ANTITHROHBIN A FOLLOWING THROMBIN HIRUDIN AS AN INHIBITOR
USING
A.K.
O.H.
Haaland,
Haematological
Research
Ullevil
(Received
Skjansberg, and H.C.
20.6.1990;
K.
(TAT) LEVELS AND OF HUMAN PLASMA FORMATION
Gravem,
R.
Ruyter
Godal
Laboratory,
Hospital,
accepted
COMPLEX INCUBATION OF TAT
N-0407
University Oslo
4,
Clinic,
Norway
in revised form 12.11 .1990 by Editor U. Abildgaard)
ABSTRACT Following thrombin, III was
incubation the interaction measured
concentration. on fibrinogen,
plasma
as thrombin-antithrombin Comparison was made to assayed
scattering
titrated of thrombin
of
as
with
with
human
antithrombin
complex (TAT) thrombin activity
fibrinopeptide
A
(FPA).
Light
were included to evaluate polymeriat a final concentration of Hirudin, zation of fibrin. effectively inhibited TAT generation at final 100 U/ml, thrombin concentrations below 0.4 NIH U/ml. Hirudin by itself did not affect the TAT ELISA analysis. TAT and
FPA
values
equally
studies
correlated represent
well
closely in
(r=0.83, p
COMPARISON FIBRINOPEPTIDE
OF
THROMBIN-ANTITHROHBIN A FOLLOWING THROMBIN HIRUDIN AS AN INHIBITOR
USING
A.K.
O.H.
Haaland,
Haematological
Research
Ullevil
(Received
Skjansberg, and H.C.
20.6.1990;
K.
(TAT) LEVELS AND OF HUMAN PLASMA FORMATION
Gravem,
R.
Ruyter
Godal
Laboratory,
Hospital,
accepted
COMPLEX INCUBATION OF TAT
N-0407
University Oslo
4,
Clinic,
Norway
in revised form 12.11 .1990 by Editor U. Abildgaard)
ABSTRACT Following thrombin, III was
incubation the interaction measured
concentration. on fibrinogen,
plasma
as thrombin-antithrombin Comparison was made to assayed
scattering
titrated of thrombin
of
as
with
with
human
antithrombin
complex (TAT) thrombin activity
fibrinopeptide
A
(FPA).
Light
were included to evaluate polymeriat a final concentration of Hirudin, zation of fibrin. effectively inhibited TAT generation at final 100 U/ml, thrombin concentrations below 0.4 NIH U/ml. Hirudin by itself did not affect the TAT ELISA analysis. TAT and
FPA
values
equally
studies
correlated represent
well
closely in
(r=0.83, p
