_~ ~ 360
©D) 1990 Oxford University Press
2822 Nucleic Acids Research, Vol. 18, No. 9
Complete cDNA sequence encoding rat high and low molecular weight MAP2 Stefan Kindler, Birgit Schwanke, Barbara Schulz and Craig C.Garner Center for Molecular Neurobiology, University of Hamburg, Martinistrasse 52, 2000 Hamburg 20, FRG Submitted February 22, 1990
Embryonic and early postnatal rat brain express a high and low molecular weight form of MAP2. Although the larger protein, MAP2b, is encoded on a 9 kb mRNA and the smaller form, MAP2c, by a 6 kb mRNA, both are transcribed from a single gene (1). Here, we present the nucleotide sequences spanning the coding regions for these two proteins. These were obtained by sequencing two sets of overlapping cDNA clones isolated from a postnatal day 5 rat brain cDNA library. The underlined nucleotide sequences are from the MAP2c cDNA clones. The derived amino acid sequence for the rat high molecular weight (HMW) MAP2 is 1830 amino acids long (Mr = 199.1 kDa), whereas MAP2c contains 467 amino acids (Mr = 42.3 kDa). MAP2c has an identical protein and nucleic acid sequence to the HMW MAP2 with the exception that an internal block of 1363 amino acids from HMW MAP2 have been deleted. This demonstrates that MAP2C is generated via alternative splicing of a primary MAP2 transcript. fCA!&TAACAAACGTCATTACTTT,ACAACGATTAGGAGACAGTACAGAGZATCTGAAGGk
60
=
_
CAGCTCTGCCTTAGCAGCTGAAGAAACAGTTAATCTGCCACCTTCCCCACCACCATCGC CAGCATCAGAACAAACAGCTGCACTGQAAGAAGATACTTACAGCCTCGAAGATGGAAT
TCCCTGAGCAGCAGAAATTGCCTTCCTCATTCGCTGAGCCTTTAGACAAGGAGGAAACGG AGTTTAAGATGCAAAGTAAGCCTGGTGAAGACTTTGAACACGCTGCCTTAGTTCCTCAGC CGGACACAAGTAAAACTCCCCAGGATAAAAAGGATCCCCAAGACATGGAAGGAGAAAAGT
CGCCTGCCAGTCCATTTGCGCAGACTTTCGGTACCAACCTGGAAGACATAAAACAGATCA CAGAACCAAGCATAACAGTACCTAGCATTGGCCTCTCCGCAGAGCCCCTAGCTCCAAAAG ATCAGAAAGACTGGTTCATCGAAATGCCCGTGGAATCAAAGAAGGATGAATGGGGTTTAG CTGCCCCAATATCTCCTGGCCCCTTGACACCCATGAGGGAAAAAGATGTGCTGGAGGATA
TCCCAAGATGGGAAGGAAAGCAGTTTGACTCTCCCATGCCTAGCCCCTTCCACGGTGGAA GTTTCACTCTTCCCTTAGATACTGTGAAAGATGAGAGAGTCACAGAAGGGTCACAACCCT
TTGCCCCTGTCTTCTTCCAATCAGATGACAAAATGTCTCTGCAGGACACCAGTGGTTCAG CTACTTCCAAAGAGAGTTCTAAAGATGAAGAGCCACAGAAAGATAAAGCAGACAAAGTGG CAGATGTTCCTGTCTCAGAAGCTACCACTGTACTGGGAGATGTTCACAGTCCAGCTGTGG AAGGCTTTGTCGGGGAGAACATTTCAGGAGAAGAAAAGGGTACCACAGATCAAGAGAAAA
AAGAGACTTCGACACCCAGTGTACAGGAACCTACACTCACTGAAACTGAACCACAGACAA AGCTTGAAGAGACATCAAAGGTTTCCATCGAAGAAACTGTGGCAAAAGAAGAGGAATCCT
TGAAATTAAAAGATGATAAAGCAGGTGTAATTCAGACTTCCACCGAGCAGTCTTTCTCCA AAGAAGACCAGAAAGGCCAAGAACAGACAATCGAAGCATTAAAACAAGACTCCTTTCCTA TAAGTCTAGAACAGGCAGTTACAGATGCAGCCATGGCCACCAAGACCTTGGAAAAGGTTA CGTCTGAGCCAGAGGCAGTAAGTGAAAAGAGAGAAATCCAGGGACTTTTTGAAGAGGATA TAGCTGACAAGAGTAAGCTCGAAGGCGCTGGGTCTGCAACAGTAGCCGAGGTTGAGATGC CATTTTATGAAGATAAATCAGGGATGTCCAAGTACTTTGAAACATCTGCATTGAAAGAAG ATGTGACCAGAAGCACTGGGTTGGGCAGTGATTACTACGAGCTGAGTGACTCAAGAGGAA ATGCCCAGGAATCTCTTGATACTGTATCTCCCAAGAACCAACAAGATGAAAAGGAACTTC TGGCAAAAGCTTCCCAGCCTAGTCCTCCAGCACACGAAGCAGGGTACAGCACTCTTGCCC AGAGTTATACATCTGATCATCCGTCCGAGTTACCTGAAGAACCAAGTTCTCCTCAAGAAA GAATGTTCACTATTGACCCCAAAGTTTATGGGGAGAAAAGGGACCTTCATAGTAAGAACA
AAGATGATCTGACACTTAGTCGAAGCTTGGGGCTGGGTGGAAGGTCTGCAATAGAACAGA GAAGCATGTCCATTAACTTGCCTATGTCTTGCCTTGATTCTATTGCCCTTGGGTTTAACT TTGGCCGGGGCCATGATCTTTCCCCTCTGGCTTCTGATATTCTAACCAACACTAGCGGAA GCATGGATGAAGGAGATGATTACCTGCCCCCCACCACACCTGCAGTGGAGAAGATTCCTT GCTTTCCAATAGAGAGCAAAGAGGAAGAAGATAAGACAGAGCAAGCAAAAGTGACTGGAG GGCAAACTACCCAAGTTGAAACATCCTCCGAGTCACCCTTCCCAGCCAAAGAATATTACA AAAATGGCACTGTCATGGCCCCTGACCTGCCTGAGATGCTAGATCTAGCAGGAACCAGGT CCAGATTAGCTTCTGTGAGTGCAGATGCTGAGGTTGCCAGGAGGAAATCAGTCCCATCGG AGGCTGTGGTTGCAGAGAGCAGTACTGGTTTGCCACCTGTTGCTGATGACAGCCAACCCG TAAAACCAGACAGTCAACTTGAAGACATGGGGTACTGTGTGTTCAACAAGTACACAGTCC CTCTCCCATCGCCAGTTCAAGACAGTGAGAATTTGTCAGGAGAGAGTGGTTCGTTTTATG AAGGAACCGATGACAAAGTCCGTAGAGATTTGGCCACTGACCTTTCACTAATTGAGGTAA AACTTGCAGCTGCTGGAAGAGTCAAAGATGAATTCACTGCTGAGAAAGAGGCATCTCCAC CCTCTTCTGCTGACAAATCAGGACTGAGTAGGGAGTTTGACCAAGACAGGAAAGCTAATG
The rat HMW MAP2 nucleotide and protein sequences were found to have 93.4% and 92% homology, respectively, to the mouse HMW MAP2 (2). Rat HMW MAP2 and MAP2c both contain the three 18 amino acid repeats found to be responsible for the microtubule binding of variant tau forms (3) and mouse HMW MAP2 (4).
ACKNOWLEDGEMENT This work forms part of a doctoral thesis of S.Kindler.
REFERENCES 1. 2. 3. 4.
Garner,C.C. and Matus,A. (1988) J. Cell Biol. 106, 779-783. Wang,D., Lewis,S.A. and Cowan,N.J. (1988) Nucl. Acids Res. 16, 11369. Lewis,S.A., Wang,D. and Cowan,N.J. (1988) Science 242, 936-939. Lee,G., Neve,R.L. and Kosik,K.S. (1989) Neuron, 2, 1615-1624.
ACAAGCTGGATACTGTCCTAGAAAAGAGCGAAGAGCATGTTGATTCAAAAGAACATGCCA 3000 AGGAGTCAGAAGAGGTTGGGGATAAAGTAGAGCTCTTOGGATTAGGTGTAACCTATGAGC 3060 AAACCTCTGCCAAAGAACTGATAACAACTAAAGAAACAGChCTGAGAGAGCAGAGAAAG 3120 GTCTCAGTTCAGTGCCAGAGGTAGCTGAGG'AGAMCAACCACAAAAGCTGACCAAGGTC 3180 ~~~~~~~~~~~~~~~~300 TAGATGTTGCTGCCAAGAAAGATGATCAGAGTCCATTAGATATAAAAGTCAGTGACTTTG 3240 GACAGATGGCTTCTGGGATGAGTGTAGATGCTGGGAAAACCATAGAGCTTAAGTTCGAGG 3300 42 0 TTGATCAGCAGCTGACTCTCTCATCCGAAGCACCTCAGGAAACAGATTCATTCATGGGTA 3360 480 TTGAGTCCAGCCACGTGAAGGATGGTGCCAAAGTCAGTGAAACAGAAGTCAAAGAGAAGG 3420 540 TGGCAAAGCCTGACTTGGTGCATCAGGAGGCTGTGGACAAAGAAGAGTCCTATGAGTCTA 3480 600 GTGGTGAGCATGAAAGCCTCACCATGGAGTCCCTGAAGCCTGATGAGGGCAAGAAAGAAA 3540 660 CATCTCCAGAGACATCACTGATACAAGATGAAGTTGCCCTCAAACTGTCTGTAGAAATCC 3600 720 CTTGCCCACCTCCAGTTTCCGAAGCTGATTCATCCATTGATGAGAAGGCGGAGGTCCAGA 3660 780 TGGAATTTATTCAGCTGCCAAAGGAAGAGAGCACAGAGACTCCGGATATACCTGCCATAC 3720 840 CTTCTGATGTCACCCAGCCACAGCCTGAAGCAGTTGTGTCCGAACCAGCAGAGGTTCGAG 3780 900 GTGAGGAAGAAGAGATCGAAGCTGAGGGAGAATATGACAAACTGCTCTTCCGCTCAGACA 3840 960 CCCTCCAGATCACCGACCTGCTTGTTCCAGGAAGTAGGGAGGAGTTTGTGGAGACCTGCC 3900 1020 CAGGGGAGCACAAAGGTGTGGTTGAGTCCGTGGTAACCATCGAGGATGATTTCATCACTG 3960 1080 TAGTACAAACCACGACTGATGAGGGAGAGTTGGGATCCCACAGTGTGCGCTTTGCAGCTC 4020 1140 CAGTTCAGCCTGAGGAAGAAAGGAGACCATACCCTCATGATGAAGAGCTTGAAGTACTGA 4080 1200 TGGCAGCAGAAGCCCAGGCAGAGCCCAAGGATGGCTCTCCAGATGCTCCAGCTACCCCTG 4140 1260 AGAAAGAAGAGGTTCCATTCTCAGAATATAAAACAGAAACCTACGACGATTACAAAGATG 4200 1320 AGACCACCATTGATGACTCCATTATGGATGCCGACAGCCTGTGGGTGGACACTCAAGATG 4260 1380 ATGATAGAAGCATCTTGACAGAGCAGTTAGAAACTATTCCTAAAGAGGAGAGAGCTGAGA 4320 1440 AGGAAGCTCGGAGACCGTCTCTCGAGAAACATAGAAAAGAAAAACCTTTTAAAACTGGGA 4380 1500 GAGGCAGAATTTCCACTCCTGAAAGAAGAGAAGTAGCTAAAAAGGAACCTAGCACGGTCT 4440 1560 CCAGGGATGAAGTGAGAAGGAAAAAAGCAGTTTATAAGAAGGCTGAACTTGCTAAAGAAT 4500 1620 CAGAAGTTCAGGCCCACTCTCCTTCCAGGAAACTCATTTTAAAACCTGCTATCAAATACA 4560 1680 CTAGACCAACTCATCTCTCCTGTGTTAAGCGGAAAACCACAGCAACAAGTGGTGAATCAG 4620 1740 CTCAGGCTCCCAGTGCGTTTAAACAGGCGAAGGACAAAGTCACTGATGGAATAACCAAGA 4680 1800 GCCCAG,AAAAACGTTCTTCCCTCCCAAGACCTTCCTCCATCCTCCCTCCTCGCAGGGGCG 4740 1860 TATCAGGAGACAGGGAGGAGAACTCGTTCTCTCTGAACAGCTCCATCTCTTCAGCACGAC 4800 1920 GGACCACCAGGTCAGAACCAATTCGCAGAGCAGGAAAAAGCGGCACCTCAACACCTACTA 4860 1980 CCCCTGGATCTACTGCAATCACCCCTGGCACTCCTCCAAGCTACTCTTCACGTACCCCAG 4920 2040 GCACCCCTGGAACCCCGAGCTATCCCAGGACACCAGGAACCCCCAAATCTGGCATCTTGG 4980 2100 TGCCCAGTGAGAAGAAAGTTGCCATCATTCGCACTCCTCCAAAGTCCCCAGCTACTCCCA 5040 2160 AGCAGCTTCGGCTCATTAACCAACCTCTGCCAGACCTGAAGAACGTCAAGTCCAAAATCG 5100 2220 GATCAACCGACAACATCAAATACCAGCCTAAGGGGGGTCAGGTACAA&TTGTTACTAAGA 5160 2280 AGATAGACTTAAGCCATGTGACTTCCAAATGTGGCTCTCT,AAAGAACATCCGTCACAGGC 5220 2340 CAGGTGGTGGACGCGTGAAGATTGAGAGTGTAAAGCTGGATTTCAAGGAGAAGGCCCAAG 5280 2400 CTAAAGTTGGCTCACTTGACAATGCTCACCATGTACCTGGAGGTGGrTAACGTGAAGATG 5340 2460 ACAGCCAAAAGCTGAACTTCCGAGAGCATGCAAAGGCCCGCGTCGAcCGGCTGAGA 5400 2520 TCATCACACAGTCGCCAAGCAGGTCAAGCGTGGCGTCTCCCCGGCGACTCAGCAATGTCT 5460 2580 CCTCTTCTGGAAGCATCAACCTGCTCGAA'CCCCTCAGCTGGCCACTTTGGCTGAGGACG 5520 2640 TCACTGCGGCGCTCGCTAAGCAGGGCTTGT-IATCCTTCTCATCTAGCACTGAKGTACCA 5580 2700 TTTAGGCATGAGCTCTTGGCAGGAGTGGGCTCTGAGCAGGTGTCATATTCATTCTTTACA 5640 2760 AACCATAGATAAATAATCTCATCCCCCAACTGTAGTAATTGTTACAATTTTATATAAAAA 5700 2820 5702 A& 2880 2940
_9CTGA _AAGACGAAGG&MGQCACCACACTGGACATCAGCCTCACTCACAG 120 AGGCAGCTGCACACCCCCACTCGCCAGAGATGAAGGACCAGGGTGGCTCAGGGGAAGGGC 180 TgAGCCGCAGCGCCAATGGATTTCC,ATA,GAGAGGAGGAGGAAGGCGCCTTTGGGGAGCw 2240 AAGCTG,TAGCAGTCCTGAAAGGTGAACAAGAGAAGGAGGCCCAACACAAGGATCAG,T
EMBL accession no. X51842
©D) 1990 Oxford University Press
2822 Nucleic Acids Research, Vol. 18, No. 9
Complete cDNA sequence encoding rat high and low molecular weight MAP2 Stefan Kindler, Birgit Schwanke, Barbara Schulz and Craig C.Garner Center for Molecular Neurobiology, University of Hamburg, Martinistrasse 52, 2000 Hamburg 20, FRG Submitted February 22, 1990
Embryonic and early postnatal rat brain express a high and low molecular weight form of MAP2. Although the larger protein, MAP2b, is encoded on a 9 kb mRNA and the smaller form, MAP2c, by a 6 kb mRNA, both are transcribed from a single gene (1). Here, we present the nucleotide sequences spanning the coding regions for these two proteins. These were obtained by sequencing two sets of overlapping cDNA clones isolated from a postnatal day 5 rat brain cDNA library. The underlined nucleotide sequences are from the MAP2c cDNA clones. The derived amino acid sequence for the rat high molecular weight (HMW) MAP2 is 1830 amino acids long (Mr = 199.1 kDa), whereas MAP2c contains 467 amino acids (Mr = 42.3 kDa). MAP2c has an identical protein and nucleic acid sequence to the HMW MAP2 with the exception that an internal block of 1363 amino acids from HMW MAP2 have been deleted. This demonstrates that MAP2C is generated via alternative splicing of a primary MAP2 transcript. fCA!&TAACAAACGTCATTACTTT,ACAACGATTAGGAGACAGTACAGAGZATCTGAAGGk
60
=
_
CAGCTCTGCCTTAGCAGCTGAAGAAACAGTTAATCTGCCACCTTCCCCACCACCATCGC CAGCATCAGAACAAACAGCTGCACTGQAAGAAGATACTTACAGCCTCGAAGATGGAAT
TCCCTGAGCAGCAGAAATTGCCTTCCTCATTCGCTGAGCCTTTAGACAAGGAGGAAACGG AGTTTAAGATGCAAAGTAAGCCTGGTGAAGACTTTGAACACGCTGCCTTAGTTCCTCAGC CGGACACAAGTAAAACTCCCCAGGATAAAAAGGATCCCCAAGACATGGAAGGAGAAAAGT
CGCCTGCCAGTCCATTTGCGCAGACTTTCGGTACCAACCTGGAAGACATAAAACAGATCA CAGAACCAAGCATAACAGTACCTAGCATTGGCCTCTCCGCAGAGCCCCTAGCTCCAAAAG ATCAGAAAGACTGGTTCATCGAAATGCCCGTGGAATCAAAGAAGGATGAATGGGGTTTAG CTGCCCCAATATCTCCTGGCCCCTTGACACCCATGAGGGAAAAAGATGTGCTGGAGGATA
TCCCAAGATGGGAAGGAAAGCAGTTTGACTCTCCCATGCCTAGCCCCTTCCACGGTGGAA GTTTCACTCTTCCCTTAGATACTGTGAAAGATGAGAGAGTCACAGAAGGGTCACAACCCT
TTGCCCCTGTCTTCTTCCAATCAGATGACAAAATGTCTCTGCAGGACACCAGTGGTTCAG CTACTTCCAAAGAGAGTTCTAAAGATGAAGAGCCACAGAAAGATAAAGCAGACAAAGTGG CAGATGTTCCTGTCTCAGAAGCTACCACTGTACTGGGAGATGTTCACAGTCCAGCTGTGG AAGGCTTTGTCGGGGAGAACATTTCAGGAGAAGAAAAGGGTACCACAGATCAAGAGAAAA
AAGAGACTTCGACACCCAGTGTACAGGAACCTACACTCACTGAAACTGAACCACAGACAA AGCTTGAAGAGACATCAAAGGTTTCCATCGAAGAAACTGTGGCAAAAGAAGAGGAATCCT
TGAAATTAAAAGATGATAAAGCAGGTGTAATTCAGACTTCCACCGAGCAGTCTTTCTCCA AAGAAGACCAGAAAGGCCAAGAACAGACAATCGAAGCATTAAAACAAGACTCCTTTCCTA TAAGTCTAGAACAGGCAGTTACAGATGCAGCCATGGCCACCAAGACCTTGGAAAAGGTTA CGTCTGAGCCAGAGGCAGTAAGTGAAAAGAGAGAAATCCAGGGACTTTTTGAAGAGGATA TAGCTGACAAGAGTAAGCTCGAAGGCGCTGGGTCTGCAACAGTAGCCGAGGTTGAGATGC CATTTTATGAAGATAAATCAGGGATGTCCAAGTACTTTGAAACATCTGCATTGAAAGAAG ATGTGACCAGAAGCACTGGGTTGGGCAGTGATTACTACGAGCTGAGTGACTCAAGAGGAA ATGCCCAGGAATCTCTTGATACTGTATCTCCCAAGAACCAACAAGATGAAAAGGAACTTC TGGCAAAAGCTTCCCAGCCTAGTCCTCCAGCACACGAAGCAGGGTACAGCACTCTTGCCC AGAGTTATACATCTGATCATCCGTCCGAGTTACCTGAAGAACCAAGTTCTCCTCAAGAAA GAATGTTCACTATTGACCCCAAAGTTTATGGGGAGAAAAGGGACCTTCATAGTAAGAACA
AAGATGATCTGACACTTAGTCGAAGCTTGGGGCTGGGTGGAAGGTCTGCAATAGAACAGA GAAGCATGTCCATTAACTTGCCTATGTCTTGCCTTGATTCTATTGCCCTTGGGTTTAACT TTGGCCGGGGCCATGATCTTTCCCCTCTGGCTTCTGATATTCTAACCAACACTAGCGGAA GCATGGATGAAGGAGATGATTACCTGCCCCCCACCACACCTGCAGTGGAGAAGATTCCTT GCTTTCCAATAGAGAGCAAAGAGGAAGAAGATAAGACAGAGCAAGCAAAAGTGACTGGAG GGCAAACTACCCAAGTTGAAACATCCTCCGAGTCACCCTTCCCAGCCAAAGAATATTACA AAAATGGCACTGTCATGGCCCCTGACCTGCCTGAGATGCTAGATCTAGCAGGAACCAGGT CCAGATTAGCTTCTGTGAGTGCAGATGCTGAGGTTGCCAGGAGGAAATCAGTCCCATCGG AGGCTGTGGTTGCAGAGAGCAGTACTGGTTTGCCACCTGTTGCTGATGACAGCCAACCCG TAAAACCAGACAGTCAACTTGAAGACATGGGGTACTGTGTGTTCAACAAGTACACAGTCC CTCTCCCATCGCCAGTTCAAGACAGTGAGAATTTGTCAGGAGAGAGTGGTTCGTTTTATG AAGGAACCGATGACAAAGTCCGTAGAGATTTGGCCACTGACCTTTCACTAATTGAGGTAA AACTTGCAGCTGCTGGAAGAGTCAAAGATGAATTCACTGCTGAGAAAGAGGCATCTCCAC CCTCTTCTGCTGACAAATCAGGACTGAGTAGGGAGTTTGACCAAGACAGGAAAGCTAATG
The rat HMW MAP2 nucleotide and protein sequences were found to have 93.4% and 92% homology, respectively, to the mouse HMW MAP2 (2). Rat HMW MAP2 and MAP2c both contain the three 18 amino acid repeats found to be responsible for the microtubule binding of variant tau forms (3) and mouse HMW MAP2 (4).
ACKNOWLEDGEMENT This work forms part of a doctoral thesis of S.Kindler.
REFERENCES 1. 2. 3. 4.
Garner,C.C. and Matus,A. (1988) J. Cell Biol. 106, 779-783. Wang,D., Lewis,S.A. and Cowan,N.J. (1988) Nucl. Acids Res. 16, 11369. Lewis,S.A., Wang,D. and Cowan,N.J. (1988) Science 242, 936-939. Lee,G., Neve,R.L. and Kosik,K.S. (1989) Neuron, 2, 1615-1624.
ACAAGCTGGATACTGTCCTAGAAAAGAGCGAAGAGCATGTTGATTCAAAAGAACATGCCA 3000 AGGAGTCAGAAGAGGTTGGGGATAAAGTAGAGCTCTTOGGATTAGGTGTAACCTATGAGC 3060 AAACCTCTGCCAAAGAACTGATAACAACTAAAGAAACAGChCTGAGAGAGCAGAGAAAG 3120 GTCTCAGTTCAGTGCCAGAGGTAGCTGAGG'AGAMCAACCACAAAAGCTGACCAAGGTC 3180 ~~~~~~~~~~~~~~~~300 TAGATGTTGCTGCCAAGAAAGATGATCAGAGTCCATTAGATATAAAAGTCAGTGACTTTG 3240 GACAGATGGCTTCTGGGATGAGTGTAGATGCTGGGAAAACCATAGAGCTTAAGTTCGAGG 3300 42 0 TTGATCAGCAGCTGACTCTCTCATCCGAAGCACCTCAGGAAACAGATTCATTCATGGGTA 3360 480 TTGAGTCCAGCCACGTGAAGGATGGTGCCAAAGTCAGTGAAACAGAAGTCAAAGAGAAGG 3420 540 TGGCAAAGCCTGACTTGGTGCATCAGGAGGCTGTGGACAAAGAAGAGTCCTATGAGTCTA 3480 600 GTGGTGAGCATGAAAGCCTCACCATGGAGTCCCTGAAGCCTGATGAGGGCAAGAAAGAAA 3540 660 CATCTCCAGAGACATCACTGATACAAGATGAAGTTGCCCTCAAACTGTCTGTAGAAATCC 3600 720 CTTGCCCACCTCCAGTTTCCGAAGCTGATTCATCCATTGATGAGAAGGCGGAGGTCCAGA 3660 780 TGGAATTTATTCAGCTGCCAAAGGAAGAGAGCACAGAGACTCCGGATATACCTGCCATAC 3720 840 CTTCTGATGTCACCCAGCCACAGCCTGAAGCAGTTGTGTCCGAACCAGCAGAGGTTCGAG 3780 900 GTGAGGAAGAAGAGATCGAAGCTGAGGGAGAATATGACAAACTGCTCTTCCGCTCAGACA 3840 960 CCCTCCAGATCACCGACCTGCTTGTTCCAGGAAGTAGGGAGGAGTTTGTGGAGACCTGCC 3900 1020 CAGGGGAGCACAAAGGTGTGGTTGAGTCCGTGGTAACCATCGAGGATGATTTCATCACTG 3960 1080 TAGTACAAACCACGACTGATGAGGGAGAGTTGGGATCCCACAGTGTGCGCTTTGCAGCTC 4020 1140 CAGTTCAGCCTGAGGAAGAAAGGAGACCATACCCTCATGATGAAGAGCTTGAAGTACTGA 4080 1200 TGGCAGCAGAAGCCCAGGCAGAGCCCAAGGATGGCTCTCCAGATGCTCCAGCTACCCCTG 4140 1260 AGAAAGAAGAGGTTCCATTCTCAGAATATAAAACAGAAACCTACGACGATTACAAAGATG 4200 1320 AGACCACCATTGATGACTCCATTATGGATGCCGACAGCCTGTGGGTGGACACTCAAGATG 4260 1380 ATGATAGAAGCATCTTGACAGAGCAGTTAGAAACTATTCCTAAAGAGGAGAGAGCTGAGA 4320 1440 AGGAAGCTCGGAGACCGTCTCTCGAGAAACATAGAAAAGAAAAACCTTTTAAAACTGGGA 4380 1500 GAGGCAGAATTTCCACTCCTGAAAGAAGAGAAGTAGCTAAAAAGGAACCTAGCACGGTCT 4440 1560 CCAGGGATGAAGTGAGAAGGAAAAAAGCAGTTTATAAGAAGGCTGAACTTGCTAAAGAAT 4500 1620 CAGAAGTTCAGGCCCACTCTCCTTCCAGGAAACTCATTTTAAAACCTGCTATCAAATACA 4560 1680 CTAGACCAACTCATCTCTCCTGTGTTAAGCGGAAAACCACAGCAACAAGTGGTGAATCAG 4620 1740 CTCAGGCTCCCAGTGCGTTTAAACAGGCGAAGGACAAAGTCACTGATGGAATAACCAAGA 4680 1800 GCCCAG,AAAAACGTTCTTCCCTCCCAAGACCTTCCTCCATCCTCCCTCCTCGCAGGGGCG 4740 1860 TATCAGGAGACAGGGAGGAGAACTCGTTCTCTCTGAACAGCTCCATCTCTTCAGCACGAC 4800 1920 GGACCACCAGGTCAGAACCAATTCGCAGAGCAGGAAAAAGCGGCACCTCAACACCTACTA 4860 1980 CCCCTGGATCTACTGCAATCACCCCTGGCACTCCTCCAAGCTACTCTTCACGTACCCCAG 4920 2040 GCACCCCTGGAACCCCGAGCTATCCCAGGACACCAGGAACCCCCAAATCTGGCATCTTGG 4980 2100 TGCCCAGTGAGAAGAAAGTTGCCATCATTCGCACTCCTCCAAAGTCCCCAGCTACTCCCA 5040 2160 AGCAGCTTCGGCTCATTAACCAACCTCTGCCAGACCTGAAGAACGTCAAGTCCAAAATCG 5100 2220 GATCAACCGACAACATCAAATACCAGCCTAAGGGGGGTCAGGTACAA&TTGTTACTAAGA 5160 2280 AGATAGACTTAAGCCATGTGACTTCCAAATGTGGCTCTCT,AAAGAACATCCGTCACAGGC 5220 2340 CAGGTGGTGGACGCGTGAAGATTGAGAGTGTAAAGCTGGATTTCAAGGAGAAGGCCCAAG 5280 2400 CTAAAGTTGGCTCACTTGACAATGCTCACCATGTACCTGGAGGTGGrTAACGTGAAGATG 5340 2460 ACAGCCAAAAGCTGAACTTCCGAGAGCATGCAAAGGCCCGCGTCGAcCGGCTGAGA 5400 2520 TCATCACACAGTCGCCAAGCAGGTCAAGCGTGGCGTCTCCCCGGCGACTCAGCAATGTCT 5460 2580 CCTCTTCTGGAAGCATCAACCTGCTCGAA'CCCCTCAGCTGGCCACTTTGGCTGAGGACG 5520 2640 TCACTGCGGCGCTCGCTAAGCAGGGCTTGT-IATCCTTCTCATCTAGCACTGAKGTACCA 5580 2700 TTTAGGCATGAGCTCTTGGCAGGAGTGGGCTCTGAGCAGGTGTCATATTCATTCTTTACA 5640 2760 AACCATAGATAAATAATCTCATCCCCCAACTGTAGTAATTGTTACAATTTTATATAAAAA 5700 2820 5702 A& 2880 2940
_9CTGA _AAGACGAAGG&MGQCACCACACTGGACATCAGCCTCACTCACAG 120 AGGCAGCTGCACACCCCCACTCGCCAGAGATGAAGGACCAGGGTGGCTCAGGGGAAGGGC 180 TgAGCCGCAGCGCCAATGGATTTCC,ATA,GAGAGGAGGAGGAAGGCGCCTTTGGGGAGCw 2240 AAGCTG,TAGCAGTCCTGAAAGGTGAACAAGAGAAGGAGGCCCAACACAAGGATCAG,T
EMBL accession no. X51842
