Nucleic Acids Research, Vol. 18, No. 23 7135
Complete nucleotide cDNA
sequence of a
pigeon alpha-globin
Yukinori Eguchi, Yasutsugu Nakashima, Minoru Oshiro and Hiroshi Takei Department of Biochemistry, University of the Ryukyus, Faculty of Medicine, Nishihara-Cho, Okinawa 903-01, Japan EMBL accession no. X56349
Submitted October 31, 1990
Most adult aves have a major and a minor hemoglobin component associated with distinct alpha-chains. As only one type of hemoglobin is found in the adult pigeon, the possibility that the pigeon alpha-globin gene system differs from that of other avian species had to be considered. We constructed a cDNA library from the anemic red blood cells of adult pigeons and isolated an alpha-globin clone, using quail alpha-globin cDNA as a probe (1). Figure 1 presents the nucleotide sequence of the cDNA which contains an open reading frame encoding 141 amino acids of the alpha-chain. The deduced amino acid sequence shows some similarities to that of chicken alpha-globin (2-4). Pletnev et al. reported the partial nucleotide sequence of a pigeon alpha-globin cDNA which lacks the information near the carboxyterminal end of the polypeptide (5). The sequence largely matches our results.
REFERENCES 1. Oshiro,M., Eguchi,Y., Toda,T., Takei,H. and Nakashima,Y. (1989) Acta Med. Nagasaki 34, 18-23. 2. Matsuda,G., Takei,H., Wu,K.C. and Shiozawa,T. (1971) Int. J. Protein Res. 3, 173-174. 3. Deacon,N.J., Shine,J. and Naora,H. (1980) Nuci. Acids Res. 8, 1187-1199. 4. Liu,A.Y. and Salser,W. (1981) Gene 13, 409-415. 5. Pletnev,A.G., Scobeleva,N.A., Frolova,L.Y. and Kisselev,L.L. (1983) Gene
25, 93-100.
1
cgcgaaggtgacaccatg GTG CTG TCT GCC AAC GAC AAG AGC AAC GTG AAG Val Leu Ser Ala Asn Asp Lys Ser Asn Val Lys
52
12
GCC GTC FTC GCG AM ATC GGC GGC CAG GCC GGT GAC TTG GGT GGT GAA Ala Val Phe Ala Lys Ile Gly Gly Gln Ala Gly Asp Leu Gly Gly Glu
100 28
GCC CTG GAG AGG TTG TTC ATC ACC TAC CCC CAG ACC AAG ACC TAC FTC Ala Leu Glu Arg Leu Phe Ile Thr Tyr Pro Gln Thr Lys Thr Tyr Phe
148 44
CCC CAC FTC GAC CTG TCA CAT GGC TCC GCT CAG ATC MG GGG CAC GGC Pro His Phe Asp Leu Ser His Gly Ser Ala Gln Ile Lys Gly His Gly
196 MG MG GTG GCG GAG GCA CTG GTT GAG GCT GCC AAC CAC ATC GAT GAC 60 Lys Lys Val Ala Glu Ala Leu Val Glu Ala Ala Asn His Ile Asp Asp 244 72
ATC GCT GGT GCC CTC TCC MG CTG AGC GAC CTC CAC GCC CM AAG CTC Ile Ala Gly Ala Leu Ser Lys Leu Ser Asp Leu His Ala Gln Lys Leu
CGT GTG GAC CCC GTC MC TTC AM CTG CTG GGT CAC TGC TTC CTG Arg Val Asp Pro Val Asn Phe Lys Leu Leu Gly His Cys Phe Leu 340 GTC GTG GCC GTC CAC FTC CCC TCT CTC CTG ACC CCG GAG GTC CAT 108 Val Val Ala Val His Phe Pro Ser Leu Leu Thr Pro Glu Val His
292 92
GTG Val
GCT Ala
388 124
TCC CTG GAC MG FTTC GTG TTG GCC GTG GGC ACC GTC CTT ACT GCC AAG Ser Leu Asp Lys Phe Val Leu Ala Val Gly Thr Val Leu Thr Ala Lys
436 140
TAC CGT taagatgcggcaccatggctagagctggacacaacctgctgccagcceCtccaaca Tyr Arg
496 gtgagcaaccaaatgatctgaaataaaatctgttccatttgtgctccaaaaaaaaaaaaaaaaa
Figure 1. Nucleotide sequence of cDNA encoding the alpha chain of adult pigeon hemoglobin and the resultant deduced amino acid sequence. Asterisks above the nucleotide sequence indicate differences in our results and that of Pletnev et al. (5).
Complete nucleotide cDNA
sequence of a
pigeon alpha-globin
Yukinori Eguchi, Yasutsugu Nakashima, Minoru Oshiro and Hiroshi Takei Department of Biochemistry, University of the Ryukyus, Faculty of Medicine, Nishihara-Cho, Okinawa 903-01, Japan EMBL accession no. X56349
Submitted October 31, 1990
Most adult aves have a major and a minor hemoglobin component associated with distinct alpha-chains. As only one type of hemoglobin is found in the adult pigeon, the possibility that the pigeon alpha-globin gene system differs from that of other avian species had to be considered. We constructed a cDNA library from the anemic red blood cells of adult pigeons and isolated an alpha-globin clone, using quail alpha-globin cDNA as a probe (1). Figure 1 presents the nucleotide sequence of the cDNA which contains an open reading frame encoding 141 amino acids of the alpha-chain. The deduced amino acid sequence shows some similarities to that of chicken alpha-globin (2-4). Pletnev et al. reported the partial nucleotide sequence of a pigeon alpha-globin cDNA which lacks the information near the carboxyterminal end of the polypeptide (5). The sequence largely matches our results.
REFERENCES 1. Oshiro,M., Eguchi,Y., Toda,T., Takei,H. and Nakashima,Y. (1989) Acta Med. Nagasaki 34, 18-23. 2. Matsuda,G., Takei,H., Wu,K.C. and Shiozawa,T. (1971) Int. J. Protein Res. 3, 173-174. 3. Deacon,N.J., Shine,J. and Naora,H. (1980) Nuci. Acids Res. 8, 1187-1199. 4. Liu,A.Y. and Salser,W. (1981) Gene 13, 409-415. 5. Pletnev,A.G., Scobeleva,N.A., Frolova,L.Y. and Kisselev,L.L. (1983) Gene
25, 93-100.
1
cgcgaaggtgacaccatg GTG CTG TCT GCC AAC GAC AAG AGC AAC GTG AAG Val Leu Ser Ala Asn Asp Lys Ser Asn Val Lys
52
12
GCC GTC FTC GCG AM ATC GGC GGC CAG GCC GGT GAC TTG GGT GGT GAA Ala Val Phe Ala Lys Ile Gly Gly Gln Ala Gly Asp Leu Gly Gly Glu
100 28
GCC CTG GAG AGG TTG TTC ATC ACC TAC CCC CAG ACC AAG ACC TAC FTC Ala Leu Glu Arg Leu Phe Ile Thr Tyr Pro Gln Thr Lys Thr Tyr Phe
148 44
CCC CAC FTC GAC CTG TCA CAT GGC TCC GCT CAG ATC MG GGG CAC GGC Pro His Phe Asp Leu Ser His Gly Ser Ala Gln Ile Lys Gly His Gly
196 MG MG GTG GCG GAG GCA CTG GTT GAG GCT GCC AAC CAC ATC GAT GAC 60 Lys Lys Val Ala Glu Ala Leu Val Glu Ala Ala Asn His Ile Asp Asp 244 72
ATC GCT GGT GCC CTC TCC MG CTG AGC GAC CTC CAC GCC CM AAG CTC Ile Ala Gly Ala Leu Ser Lys Leu Ser Asp Leu His Ala Gln Lys Leu
CGT GTG GAC CCC GTC MC TTC AM CTG CTG GGT CAC TGC TTC CTG Arg Val Asp Pro Val Asn Phe Lys Leu Leu Gly His Cys Phe Leu 340 GTC GTG GCC GTC CAC FTC CCC TCT CTC CTG ACC CCG GAG GTC CAT 108 Val Val Ala Val His Phe Pro Ser Leu Leu Thr Pro Glu Val His
292 92
GTG Val
GCT Ala
388 124
TCC CTG GAC MG FTTC GTG TTG GCC GTG GGC ACC GTC CTT ACT GCC AAG Ser Leu Asp Lys Phe Val Leu Ala Val Gly Thr Val Leu Thr Ala Lys
436 140
TAC CGT taagatgcggcaccatggctagagctggacacaacctgctgccagcceCtccaaca Tyr Arg
496 gtgagcaaccaaatgatctgaaataaaatctgttccatttgtgctccaaaaaaaaaaaaaaaaa
Figure 1. Nucleotide sequence of cDNA encoding the alpha chain of adult pigeon hemoglobin and the resultant deduced amino acid sequence. Asterisks above the nucleotide sequence indicate differences in our results and that of Pletnev et al. (5).
