BIOLOGY
OF
REPRODUCTION
17,
Cytochemical
Studies
Suggested EDNA
787-795
A. ELFONT.
(1977)
of Acid
Role
for
Lysosomes
JOSEPH
Departments
Phosphatase
and
of Laboratory
Medicine
Sinai
of Detroit,
Detroit,
Michigan
Follicles:
A
in Steroidogenesis’
P. ROSZKA
Hospital
in Ovarian
MICHAEL and
J.
DIMINO2
Research,
48235
ABSTRACT Lysosomal activity in follides of ovaries of untreated and gonadotropic-treated immature rats was investigated using cytochemical techniques for acid phosphatase localization. The major site of acid phosphatase reaction product in ovaries of untreated immature rats was in autophagic vacuoles associated with numerous atretic follicles. Little reaction product was found in cells of intact preantral follicles. In contrast, antral follicles of rats treated with PMSG (20 IV of pregnant mare serum gonadotropin 54 h before killing) displayed more acid phosphatase reaction product than did preantral follicles, especially within their thecal steroidogenic cells. Craafian follicles of rats treated with PMSG-hCG (20 IV of PMSG plus 40 IV of human chorionic gonadotropin 54 h later and killed 16 h later) were characterized by large quantities of reaction product within both granulosa and thecal cells. However, follicular cells undergoing luteinization had more acid phosphatase reaction product than did any other cell type. The reaction product in all ovarian steroidogenic cells was associated predominantly with the active Golgi complex and the extensive agranular endoplasmic reticulum, with some reaction product within vesicles (
OF
REPRODUCTION
17,
Cytochemical
Studies
Suggested EDNA
787-795
A. ELFONT.
(1977)
of Acid
Role
for
Lysosomes
JOSEPH
Departments
Phosphatase
and
of Laboratory
Medicine
Sinai
of Detroit,
Detroit,
Michigan
Follicles:
A
in Steroidogenesis’
P. ROSZKA
Hospital
in Ovarian
MICHAEL and
J.
DIMINO2
Research,
48235
ABSTRACT Lysosomal activity in follides of ovaries of untreated and gonadotropic-treated immature rats was investigated using cytochemical techniques for acid phosphatase localization. The major site of acid phosphatase reaction product in ovaries of untreated immature rats was in autophagic vacuoles associated with numerous atretic follicles. Little reaction product was found in cells of intact preantral follicles. In contrast, antral follicles of rats treated with PMSG (20 IV of pregnant mare serum gonadotropin 54 h before killing) displayed more acid phosphatase reaction product than did preantral follicles, especially within their thecal steroidogenic cells. Craafian follicles of rats treated with PMSG-hCG (20 IV of PMSG plus 40 IV of human chorionic gonadotropin 54 h later and killed 16 h later) were characterized by large quantities of reaction product within both granulosa and thecal cells. However, follicular cells undergoing luteinization had more acid phosphatase reaction product than did any other cell type. The reaction product in all ovarian steroidogenic cells was associated predominantly with the active Golgi complex and the extensive agranular endoplasmic reticulum, with some reaction product within vesicles (
