Annals of Tropical Medicine & Parasitology

ISSN: 0003-4983 (Print) 1364-8594 (Online) Journal homepage: http://www.tandfonline.com/loi/ypgh19

Demonstration of antibodies in giardiasis using the immunodiffusion technique with Giardia cysts as antigen V. K. Vinayak, P. Jain & S. R. Naik To cite this article: V. K. Vinayak, P. Jain & S. R. Naik (1978) Demonstration of antibodies in giardiasis using the immunodiffusion technique with Giardia cysts as antigen, Annals of Tropical Medicine & Parasitology, 72:6, 581-582, DOI: 10.1080/00034983.1978.11719366 To link to this article: http://dx.doi.org/10.1080/00034983.1978.11719366

Published online: 14 Apr 2016.

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Ann a ls ofT ropi ca ll\l ed icine a nd Pa rasi1 ology. \ 'ol. 7'2 , !\o. G ( 197B)

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Demonstration of antibodies in giardiasis using the immunodiffusion technique with Giardia cysts as antigen Gi ardi as is is quit e common in Indi a (Pra kas h a nd Taml on, 1966; Vin aya k et a/., 1967; T a ndo n eta!., 19 72 ) . Th e in cid ence o f th e infec tio n is a ppa rentl y in c reas ing a nd it seem s a lso th a t la tent infec tions a rc beco ming frequ ent (Ridl ey a nd Ridle y, 197G) . Serum a ntibodi es in g ia rdi asis p a ti ent s have r ecentl y been d em onstra ted b y the indirec t flu o resce nt a n tibod y tes t (Ridl ey a nd Ridl ey, 19 76) a nd we now report our prelimin a ry o bserva ti o ns o n th e prec ipitin tes t u sing Giardia Iamblia cys ts as a ntige n . Bl ood sa mples were ta ken fro m II g ia rdi as is pa ti ent s, wh o ha d a longs ta nding histo ry (m ore th a n two yea rs) o f vague a bd o min a l di stu r ba nces . Three su ccess ive stool spec imens from each pa ti ent showed G. Iamblia cys ts a nd th eir clu ocl cna l aspira tes a lso co nt a in ed Giardia trophozoit es. At th e sa me tim e, blood sa mpl es fro m 16 appa rent ly hea lth y S11bj cc ts were a lso tes ted . Th eir stoo ls were nega ti ve for Giardia cys ts or tro ph ozo it es. In onl y seve n o f th ese a ppa r ent ly hea lth y subj ec ts was th e exa mining du od ena l as pira tes poss ibl e and th ese were a ll nega tive for Giardia trop hozoit cs. F ift ee n incli viclu a ls w ho had o th er pa ras iti c infec ti ons were a lso studi ed . Seven ba tches o f a ntigen were prepa red fro m Giardia Iambiia cysts. Th e cysts were coll ec ted by Ro ta tion in 33 % zin c sulph a te (Cra ig a nd F a ust, 1 ~) 7 0). Th e upper Ro ta tion laye r cont a ined m a n y cys ts a nd littl e stool d ebri s. Th e cys ts were th oro ug hl y was hed in norm a l sa line. T he fi na l d epos it was fa irl y clea r a nd co nt ai ned a la rge number o f cys ts. Th e cys ts were subj ec ted to ultraso ni ca tio n a t 213 kc fo r 10- l :i minut es. Th e so ni ca ted ma teria l was centrifuged cold a t 12 000 X g fo r 30 minut es a nd th e supern a ta nt used as crud e a ntige n. T he pro tein co nt ent was es tim a ted as 2 mg/ ml. Antigen from a faeca l suspensio n from Giardia-nega ti ve cases w:1s p re pa red simil a rl y a nd used as a con 1rol. The d oubl e-diffusion m ethod was used as d escribed b y Vin aya k et a/. ( J

Demonstration of antibodies in giardiasis using the immunodiffusion technique with Giardia cysts as antigen.

Annals of Tropical Medicine & Parasitology ISSN: 0003-4983 (Print) 1364-8594 (Online) Journal homepage: http://www.tandfonline.com/loi/ypgh19 Demons...
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