Carcinogenesis vol.13 no.10 pp.1835-1839, 1992
Detection and quantitation of dG-AAI and dA-AAI adducts by 32 P-postlabeling methods in urothelium and exfoliated cells in urine of rats treated with aristolochic acid I
R.Charles Fernando, Heinz H.Schmeiser, Werner Nicklas1 and Manfred Wiessler Institute of Toxicology and Chemotherapy and 'Central Animal Laboratories, German Cancer Research Center, Im Neuenheimer Feld 280, 6900 Heidelberg, Germany
Introduction Considerable evidence exists emphasizing that the formation of DNA adducts resulting from interactions of genotoxic chemical compounds with DNA is most probably the initiating event in chemical carcinogenesis (1-3). Analysis of DNA adducts of carcinogenic agents has emerged as a promising method widi which to monitor the exposure of humans to such compounds (4-6). Nevertheless, one of the main drawbacks in the •Abbreviations: AAI, aristolochic acid I; dG-AAI, 7(-deoxyguanosin-Af2-y!)— aristolactam I; dA-AAI, 7(-deoxyadenosin-A'6-yl)-aristolactam I; PBS, phosphate-buffered saline; PEI—cellulose, poly(ethylenimine)—cellulose; dAp, deoxyadenosine-3'-monophosphate; RAL, relative adduct labeling; CT-DNA, calf thymus DNA. © Oxford University Press
AAI Fig. 1. AAI, 8-methoxy-6-nitrophenanthro-[3,4-
Detection and quantitation of dG-AAI and dA-AAI adducts by 32 P-postlabeling methods in urothelium and exfoliated cells in urine of rats treated with aristolochic acid I
R.Charles Fernando, Heinz H.Schmeiser, Werner Nicklas1 and Manfred Wiessler Institute of Toxicology and Chemotherapy and 'Central Animal Laboratories, German Cancer Research Center, Im Neuenheimer Feld 280, 6900 Heidelberg, Germany
Introduction Considerable evidence exists emphasizing that the formation of DNA adducts resulting from interactions of genotoxic chemical compounds with DNA is most probably the initiating event in chemical carcinogenesis (1-3). Analysis of DNA adducts of carcinogenic agents has emerged as a promising method widi which to monitor the exposure of humans to such compounds (4-6). Nevertheless, one of the main drawbacks in the •Abbreviations: AAI, aristolochic acid I; dG-AAI, 7(-deoxyguanosin-Af2-y!)— aristolactam I; dA-AAI, 7(-deoxyadenosin-A'6-yl)-aristolactam I; PBS, phosphate-buffered saline; PEI—cellulose, poly(ethylenimine)—cellulose; dAp, deoxyadenosine-3'-monophosphate; RAL, relative adduct labeling; CT-DNA, calf thymus DNA. © Oxford University Press
AAI Fig. 1. AAI, 8-methoxy-6-nitrophenanthro-[3,4-
