Journal of Dermatological Science, 4 (1992)

166- 17 1

0 1992 Elsevier Science Publishers B.V. All rights reserved. 0923-1811/92/$05.00

166

DESC 00161

Detection

of human papillomavirus DNA in seborrheic polymerase chain reaction Wen-Yuan

“Department

Zhu”, Craig Leonardib

keratosis by

and Neal S. Penneys b

of Dermatology, Nanjing Medical College, Nanjing, People’s Republic of China and bDepartment of Demlatology and Cutaneous Surgerv. University of Miami School of Medicine, Miami, FL, USA

(Received 7 May 1992; accepted 20 August 1992)

Key words: Human papillomavirus;

DNA; Seborrheic keratosis; Polymerase chain reaction

Abstract

We searched for HPV DNA in 72 cases of seborrheic keratosis (nongenital SK 29, genital SK 43) using polymerase chain reaction. Amplification of HPV DNA was found in 24 (nongenital, 1; genital, 23). HPV DNA type 6 was present in 18 cases and type 11 in four and type 33 in two. Twenty-three (53%) cases of genital region SK and one (3%) case of nongenital region SK contained HPV DNA sequences. Histopathologically, the perinuclear vacuolization of epithelial cells, mitosis and dyskeratotic cells were more marked in HPV DNA-positive SK than those in negative SK (P