Scand J Clin Lab Invest 1990; 50: 587-593
Distribution of bronchoalveolar cells and fibronectin levels in bronchoalveolar lavage fluids from patients with lung disorders Scand J Clin Lab Invest Downloaded from informahealthcare.com by York University Libraries on 01/02/15 For personal use only.
B . NAKSTAD, N. P. BOYE* & T. LYBERG Departments of Pathology and Surgery and *Medicine, Ullevll University Hospital, 0407 Oslo 4, Norway
Nakstad B, Boye NP, Lyberg T. Distribution of bronchoalveolar cells and fibronectin levels in bronchoalveolar lavage fluids from patients with lung disorders. Scand J Clin Lab Invest 1990; SO: 587-593. Differential cell counts and fibronectin levels were recorded in bronchoalveolar lavage fluids (BALF) from patients with lung cancer, idiopathic pulmonary fibrosis (IPF), sarcoidosis, pneumonia, acquired immunodeficiency syndrome (AIDS), and chronic obstructive lung disease (COLD). In all groups fibronectin levels were significantly higher than in the control group; patients with sarcoidosis had a six-fold higher fibronectin level (mean values), AIDS 5.4-fold, pneumonia 4.4-fold, lung cancer, IPF and COLD 2.4-3.0-fold. In control smokers the fibronectin level was significantly higher compared to healthy nonsmokers (pO. I ) (90) 40+22 (6.5) 53? 19 ( I 10)
TOT NS
3x63 t 7 s 7 ( p0.01). No proteolytic enzyme inhibitors were added to the fresh fluid. However, all lavages were kept on ice and immediately frozen at -70 "C after sampling. Freezing and thawing up to five times did not give significantly different results when measuring fibronectin and albumin concentrations in BALF. The mean level of fibronectin was significantly higher in all patient groups than in the control group, irrespective of whether data were expressed as absolute fibronectin concentrations (nglml) or as nglmg albumin, as we have chosen. No significant differences in fibronectin levels, albumin or cell distributions were observed when the 3 x 2 0 ml lavage was compared to the total recovered lavage (3x20 m1+3x50 ml) in control subjects. In groups with COLD, IPF, and sarcoidosis the levels of albumin, fibronectin, or distribution of cells were not influenced by steroid treatment or reduced pulmonary function. The only exception was the cell distributions in patients with C O L D , as in this group a slight negative correlation (r=-0.71) between fibronectin and values of pulmonary function tests was observed. In this group (COLD), patients (n=15) with FEV,
Distribution of bronchoalveolar cells and fibronectin levels in bronchoalveolar lavage fluids from patients with lung disorders Scand J Clin Lab Invest Downloaded from informahealthcare.com by York University Libraries on 01/02/15 For personal use only.
B . NAKSTAD, N. P. BOYE* & T. LYBERG Departments of Pathology and Surgery and *Medicine, Ullevll University Hospital, 0407 Oslo 4, Norway
Nakstad B, Boye NP, Lyberg T. Distribution of bronchoalveolar cells and fibronectin levels in bronchoalveolar lavage fluids from patients with lung disorders. Scand J Clin Lab Invest 1990; SO: 587-593. Differential cell counts and fibronectin levels were recorded in bronchoalveolar lavage fluids (BALF) from patients with lung cancer, idiopathic pulmonary fibrosis (IPF), sarcoidosis, pneumonia, acquired immunodeficiency syndrome (AIDS), and chronic obstructive lung disease (COLD). In all groups fibronectin levels were significantly higher than in the control group; patients with sarcoidosis had a six-fold higher fibronectin level (mean values), AIDS 5.4-fold, pneumonia 4.4-fold, lung cancer, IPF and COLD 2.4-3.0-fold. In control smokers the fibronectin level was significantly higher compared to healthy nonsmokers (pO. I ) (90) 40+22 (6.5) 53? 19 ( I 10)
TOT NS
3x63 t 7 s 7 ( p0.01). No proteolytic enzyme inhibitors were added to the fresh fluid. However, all lavages were kept on ice and immediately frozen at -70 "C after sampling. Freezing and thawing up to five times did not give significantly different results when measuring fibronectin and albumin concentrations in BALF. The mean level of fibronectin was significantly higher in all patient groups than in the control group, irrespective of whether data were expressed as absolute fibronectin concentrations (nglml) or as nglmg albumin, as we have chosen. No significant differences in fibronectin levels, albumin or cell distributions were observed when the 3 x 2 0 ml lavage was compared to the total recovered lavage (3x20 m1+3x50 ml) in control subjects. In groups with COLD, IPF, and sarcoidosis the levels of albumin, fibronectin, or distribution of cells were not influenced by steroid treatment or reduced pulmonary function. The only exception was the cell distributions in patients with C O L D , as in this group a slight negative correlation (r=-0.71) between fibronectin and values of pulmonary function tests was observed. In this group (COLD), patients (n=15) with FEV,
