(‘ELLI’LAK
ILIMINOLOGY
Divergent
40, 154-163
(1978)
Appearance of Complement Receptors and Fc Receptors on T Cells in a Murine Mammary Tumor System
The emergence of novel T cells during nmnmary tumorig-enesis has been previously described. T cells with surfxe markers usually associated with B cells, i.e. complement receptors (CR), appear in the spleens from tumor hearing mice. \Ve now report on the appearance of Fc receptor (FcR) positive T cells in the spleens from the same animals. The kinetics of appearance of the two kinds of cells are similar. Based on evidence from double and triple label assays, it was concluded that FcR and CR arc not coexpressed on the same T cell and that the two kinds of T cells which emerge do so in an independent fashion. Furthermore. they appear to represent a branch in the differentiation process influenced by tumor growth. The development of CR+ T cells represents an irreversible process as evidenced hy the lack of change in the cells’ representation following surgical procedures. In contrast the development of FcR’ T cells appears to he quite flexible in nature since mere surgical trauma as Lvell as tumor mass removal can effect a decrease in the proportion of such cells.
During growth of mammary tumors, the host is confronted with a variety of antigenic challenges which can initiate changes in cells comprising the immune system. Differentiation then proceeds whereby virgin immune cells are sequestered and blastogenesis occurs leading to the production of immunologically active cells. Our laboratory has tlescrihetl the appearance of novel T-like splenocytes with complement receptors (CR) during tumor development ( 1 ) Their differentiation pattern appears to be altered hecause in atltlition to 1)ecoming active in cell mediated immunity they also develop smface receptors not usually associated with T lymphocytes. The emergence of these cells stimulated further interest in the charactcrimtion of spleen cells from tumor hearing mice. Previous studies revealed a significant increase in cytotoxic activity ineasuretl by antilmdy dependent cellular cytotaxicity (ADCC) (2) f a reaction mediated by Fc receptors (FcK) (3-5). The present work attempts to elucitkde the nature of the relationship lletbveen tumor burden and the appearance of lymphocyte memlxane markers which may in reality represent distinct sulq~opulations of T lymphocytes. To this end. surgical removal of tumor was performed and the resulting changes in splenocyte s~~bpop~~lations was assessed. 1.53 OOO8-8749/78/0401-0154$02.00/O Copyright 0 1978 by Academic Press, Inc. All rights of reproduction in any form reserved
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ILIMINOLOGY
Divergent
40, 154-163
(1978)
Appearance of Complement Receptors and Fc Receptors on T Cells in a Murine Mammary Tumor System
The emergence of novel T cells during nmnmary tumorig-enesis has been previously described. T cells with surfxe markers usually associated with B cells, i.e. complement receptors (CR), appear in the spleens from tumor hearing mice. \Ve now report on the appearance of Fc receptor (FcR) positive T cells in the spleens from the same animals. The kinetics of appearance of the two kinds of cells are similar. Based on evidence from double and triple label assays, it was concluded that FcR and CR arc not coexpressed on the same T cell and that the two kinds of T cells which emerge do so in an independent fashion. Furthermore. they appear to represent a branch in the differentiation process influenced by tumor growth. The development of CR+ T cells represents an irreversible process as evidenced hy the lack of change in the cells’ representation following surgical procedures. In contrast the development of FcR’ T cells appears to he quite flexible in nature since mere surgical trauma as Lvell as tumor mass removal can effect a decrease in the proportion of such cells.
During growth of mammary tumors, the host is confronted with a variety of antigenic challenges which can initiate changes in cells comprising the immune system. Differentiation then proceeds whereby virgin immune cells are sequestered and blastogenesis occurs leading to the production of immunologically active cells. Our laboratory has tlescrihetl the appearance of novel T-like splenocytes with complement receptors (CR) during tumor development ( 1 ) Their differentiation pattern appears to be altered hecause in atltlition to 1)ecoming active in cell mediated immunity they also develop smface receptors not usually associated with T lymphocytes. The emergence of these cells stimulated further interest in the charactcrimtion of spleen cells from tumor hearing mice. Previous studies revealed a significant increase in cytotoxic activity ineasuretl by antilmdy dependent cellular cytotaxicity (ADCC) (2) f a reaction mediated by Fc receptors (FcK) (3-5). The present work attempts to elucitkde the nature of the relationship lletbveen tumor burden and the appearance of lymphocyte memlxane markers which may in reality represent distinct sulq~opulations of T lymphocytes. To this end. surgical removal of tumor was performed and the resulting changes in splenocyte s~~bpop~~lations was assessed. 1.53 OOO8-8749/78/0401-0154$02.00/O Copyright 0 1978 by Academic Press, Inc. All rights of reproduction in any form reserved
.\SI)
( Ii
ITK
~I.\'I‘I'I:I.\I.s
.\Kli
anti-(‘31~1
ascities ;\ltl.
(~Kmiillgtoll.
The
Ig5l
C‘ortlis
fracticm
Ih)vine
rctl
of
I.;~l~oratori~s F:LI,',
ral)l)it
\Jdical
Sclmol.
S.\'.
])rc\~iousl!.
l,;~l~s,
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