Author's Accepted Manuscript
Exercise training combined with antioxidant supplementation prevents the antiproliferative activity of single treatment in prostate cancer through inhibition of redox adaptation Jordan Gueritat, Luz Lefeuvre-Orfila, Sophie Vincent, Armel Cretual, Jean-Luc Ravanat, Arlette Gratas-Delamarche, Françoise RannouBekono, Amélie Rebillard www.elsevier.com/locate/freeradbiomed
PII: DOI: Reference:
S0891-5849(14)00428-6 http://dx.doi.org/10.1016/j.freeradbiomed.2014.09.009 FRB12146
To appear in:
Free Radical Biology and Medicine
Received date: 6 February 2014 Revised date: 2 September 2014 Accepted date: 3 September 2014 Cite this article as: Jordan Gueritat, Luz Lefeuvre-Orfila, Sophie Vincent, Armel Cretual, Jean-Luc Ravanat, Arlette Gratas-Delamarche, Françoise RannouBekono, Amélie Rebillard, Exercise training combined with antioxidant supplementation prevents the antiproliferative activity of single treatment in prostate cancer through inhibition of redox adaptation, Free Radical Biology and Medicine, http://dx.doi.org/10.1016/j.freeradbiomed.2014.09.009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Exercise training combined with antioxidant supplementation prevents the antiproliferative activity of single treatment in prostate cancer through inhibition of redox adaptation 1
1
1
1
2
Jordan Gueritat , Luz Lefeuvre-Orfila , Sophie Vincent , Armel Cretual , Jean-Luc Ravanat , Arlette 1
1
1,
Gratas-Delamarche , Françoise Rannou-Bekono and Amélie Rebillard * 1
EA 1274, Laboratoire "Mouvement, Sport, Santé", Université de Rennes 2-ENS Cachan Antenne de
Bretagne ; Bruz, 35170, France, 2
Laboratoire « Lésions des Acides Nucléiques », Université Joseph Fourier – Grenoble 1 / CEA /
Institut Nanoscience et Cryogénie / SCIB, UMR-E3, Grenoble France * To whom correspondence should be addressed. Tel: (33) (0)2 90 09 15 87; Fax: (33) (0)2 90 09 15 80; Email: [email protected]
Abstract In preclinical models, exercise training ET or pomegranate juice PJ individually prevents prostate cancer progression. Here, we hypothesize that physical exercise combined with antioxidants could induce synergistic effects through oxidative stress modulation. Forty male Copenhagen rats with prostate tumors were divided into four groups: Control, PJ, ET, PJ+ET. Rats from PJ group consumed daily 750 μl of PJ, rats from ET group run on a treadmill 5 days per week and PJ+ET rats received the combined treatments. Each week, tumor growth was evaluated. After four weeks of treatment, rats were euthanized and blood, muscles and tumors were collected. Tumor Ki67, Extracellular signalRegulated Kinase ERK activation, Bcl2 expression, enzymatic and non-enzymatic antioxidant defenses as well as oxidative stress markers (oxidized base, lipid peroxidation, protein carbonylation) were measured. PJ or ET significantly decreased prostate tumor proliferation (Ki67 staining, p
Exercise training combined with antioxidant supplementation prevents the antiproliferative activity of single treatment in prostate cancer through inhibition of redox adaptation Jordan Gueritat, Luz Lefeuvre-Orfila, Sophie Vincent, Armel Cretual, Jean-Luc Ravanat, Arlette Gratas-Delamarche, Françoise RannouBekono, Amélie Rebillard www.elsevier.com/locate/freeradbiomed
PII: DOI: Reference:
S0891-5849(14)00428-6 http://dx.doi.org/10.1016/j.freeradbiomed.2014.09.009 FRB12146
To appear in:
Free Radical Biology and Medicine
Received date: 6 February 2014 Revised date: 2 September 2014 Accepted date: 3 September 2014 Cite this article as: Jordan Gueritat, Luz Lefeuvre-Orfila, Sophie Vincent, Armel Cretual, Jean-Luc Ravanat, Arlette Gratas-Delamarche, Françoise RannouBekono, Amélie Rebillard, Exercise training combined with antioxidant supplementation prevents the antiproliferative activity of single treatment in prostate cancer through inhibition of redox adaptation, Free Radical Biology and Medicine, http://dx.doi.org/10.1016/j.freeradbiomed.2014.09.009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Exercise training combined with antioxidant supplementation prevents the antiproliferative activity of single treatment in prostate cancer through inhibition of redox adaptation 1
1
1
1
2
Jordan Gueritat , Luz Lefeuvre-Orfila , Sophie Vincent , Armel Cretual , Jean-Luc Ravanat , Arlette 1
1
1,
Gratas-Delamarche , Françoise Rannou-Bekono and Amélie Rebillard * 1
EA 1274, Laboratoire "Mouvement, Sport, Santé", Université de Rennes 2-ENS Cachan Antenne de
Bretagne ; Bruz, 35170, France, 2
Laboratoire « Lésions des Acides Nucléiques », Université Joseph Fourier – Grenoble 1 / CEA /
Institut Nanoscience et Cryogénie / SCIB, UMR-E3, Grenoble France * To whom correspondence should be addressed. Tel: (33) (0)2 90 09 15 87; Fax: (33) (0)2 90 09 15 80; Email: [email protected]
Abstract In preclinical models, exercise training ET or pomegranate juice PJ individually prevents prostate cancer progression. Here, we hypothesize that physical exercise combined with antioxidants could induce synergistic effects through oxidative stress modulation. Forty male Copenhagen rats with prostate tumors were divided into four groups: Control, PJ, ET, PJ+ET. Rats from PJ group consumed daily 750 μl of PJ, rats from ET group run on a treadmill 5 days per week and PJ+ET rats received the combined treatments. Each week, tumor growth was evaluated. After four weeks of treatment, rats were euthanized and blood, muscles and tumors were collected. Tumor Ki67, Extracellular signalRegulated Kinase ERK activation, Bcl2 expression, enzymatic and non-enzymatic antioxidant defenses as well as oxidative stress markers (oxidized base, lipid peroxidation, protein carbonylation) were measured. PJ or ET significantly decreased prostate tumor proliferation (Ki67 staining, p
