Special issue paper Published online in Wiley Online Library
(wileyonlinelibrary.com) DOI 10.1002/bmc.3193
Dried saliva spot as a sampling technique for saliva samples Abbi Abdel-Rehima and Mohamed Abdel-Rehimb* ABSTRACT: For the first time, dried saliva spot (DSS) was used as a sampling technique for saliva samples. In the DSS technique 50 μL of saliva was collected on filter paper and the saliva was then extracted with an organic solvent. The local anesthetic lidocaine was used as a model compound, which was determined in the DSS using liquid chromatography and mass spectrometry. The results obtained for the determination of lidocaine in saliva using DSS were compared with those from a previous study using a microextraction by packed sorbent syringe as the sampling method for saliva. This study shows that DSS can be used for the analysis of saliva samples. The method is promising and very easy in terms of sampling and extraction procedures. The results from this study are in good agreement with those from our previous work on the determination of lidocaine in saliva. DSS can open a new dimension in the saliva handling process in terms of sampling, storing and transport. Copyright © 2014 John Wiley & Sons, Ltd. Keywords: saliva; dried saliva spot; lidocaine; LC/MS/MS
Introduction
Instrumentation and experiments
Drugs and metabolites can be available in human saliva. Saliva is faster and less invasive to sample from patients than plasma, making it suitable for children and elderly adults. Saliva offers unique advantages such as ease of sampling, management and transport compared with plasma or blood. Another important advantage is that there is a lower concentration of proteins in saliva than in plasma, thus reducing potential drug binding to proteins. Human saliva consists mainly of water (>99%), while the enzymes, glycoproteins and antibacterial components constitute
(wileyonlinelibrary.com) DOI 10.1002/bmc.3193
Dried saliva spot as a sampling technique for saliva samples Abbi Abdel-Rehima and Mohamed Abdel-Rehimb* ABSTRACT: For the first time, dried saliva spot (DSS) was used as a sampling technique for saliva samples. In the DSS technique 50 μL of saliva was collected on filter paper and the saliva was then extracted with an organic solvent. The local anesthetic lidocaine was used as a model compound, which was determined in the DSS using liquid chromatography and mass spectrometry. The results obtained for the determination of lidocaine in saliva using DSS were compared with those from a previous study using a microextraction by packed sorbent syringe as the sampling method for saliva. This study shows that DSS can be used for the analysis of saliva samples. The method is promising and very easy in terms of sampling and extraction procedures. The results from this study are in good agreement with those from our previous work on the determination of lidocaine in saliva. DSS can open a new dimension in the saliva handling process in terms of sampling, storing and transport. Copyright © 2014 John Wiley & Sons, Ltd. Keywords: saliva; dried saliva spot; lidocaine; LC/MS/MS
Introduction
Instrumentation and experiments
Drugs and metabolites can be available in human saliva. Saliva is faster and less invasive to sample from patients than plasma, making it suitable for children and elderly adults. Saliva offers unique advantages such as ease of sampling, management and transport compared with plasma or blood. Another important advantage is that there is a lower concentration of proteins in saliva than in plasma, thus reducing potential drug binding to proteins. Human saliva consists mainly of water (>99%), while the enzymes, glycoproteins and antibacterial components constitute
