J Huazhong Univ Sci Technol [Med Sci] 34(4):554-558,2014 DOI 10.1007/s11596-014-1314-0 J Huazhong Univ Sci Technol［Med Sci］ 34(4):2014
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E-cadherin-transfected Neural Stem Cells Transplantation for Spinal Cord Injury in Rats Chen ZHANG (张 晨)1, Feng TU (凃 峰)1, Ji-yin ZHANG (张积银)2, Lin SHEN (沈 霖)3# Department of Orthopedics, Wuhan Traditional Chinese and Western Medicine Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2 Wuhan MingRen Hospital of Traditional Chinese Medicine, Wuhan 430040, China 3 Department of Traditional Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 1
© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2014
Summary: The effects of E-cadherin-transfected neural stem cells (NSCs) transplantation for spinal cord injury (SCI) in rats were investigated. Sixty SD rats were randomly divided into model control group, NSCs group, empty plasmid group and E-cadherin overexpression group (n=15 each). The animal SCI model was established by using the modified Allen’s method. NSCs were cultured. Rats in NSCs group were subjected to NSCs transplantation. E-cadherin gene eucaryotic expression vector and pcDNA3.1-E-cadherin were respectively transfected into cultured NSCs, serving as empty plasmid group and E-cadherin overexpression group respectively. At 7th day after transplantation, neurological function of all rats was assessed by Tarlov score. After rats were sacrificed in each group, the number of BrdU and Nestin positive cells was counted by immunohistochemistry. Immumofluorescence method was used to detect the expression of neurofilament protein (NF) and glial fibrillary acidic protein (GFAP). As compared with model control group, the Tarlov score and the number of of BrdU and Nestin positive cells, and the expression of NF and GFAP in NSCs group, empty plasmid group, and E-cadherin overexpression group were increased significantly (P
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E-cadherin-transfected Neural Stem Cells Transplantation for Spinal Cord Injury in Rats Chen ZHANG (张 晨)1, Feng TU (凃 峰)1, Ji-yin ZHANG (张积银)2, Lin SHEN (沈 霖)3# Department of Orthopedics, Wuhan Traditional Chinese and Western Medicine Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2 Wuhan MingRen Hospital of Traditional Chinese Medicine, Wuhan 430040, China 3 Department of Traditional Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 1
© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2014
Summary: The effects of E-cadherin-transfected neural stem cells (NSCs) transplantation for spinal cord injury (SCI) in rats were investigated. Sixty SD rats were randomly divided into model control group, NSCs group, empty plasmid group and E-cadherin overexpression group (n=15 each). The animal SCI model was established by using the modified Allen’s method. NSCs were cultured. Rats in NSCs group were subjected to NSCs transplantation. E-cadherin gene eucaryotic expression vector and pcDNA3.1-E-cadherin were respectively transfected into cultured NSCs, serving as empty plasmid group and E-cadherin overexpression group respectively. At 7th day after transplantation, neurological function of all rats was assessed by Tarlov score. After rats were sacrificed in each group, the number of BrdU and Nestin positive cells was counted by immunohistochemistry. Immumofluorescence method was used to detect the expression of neurofilament protein (NF) and glial fibrillary acidic protein (GFAP). As compared with model control group, the Tarlov score and the number of of BrdU and Nestin positive cells, and the expression of NF and GFAP in NSCs group, empty plasmid group, and E-cadherin overexpression group were increased significantly (P
