JCM Accepted Manuscript Posted Online 7 January 2015 J. Clin. Microbiol. doi:10.1128/JCM.02648-14 Copyright © 2015, American Society for Microbiology. All Rights Reserved.
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TITLE: Early detection of the dengue virus using reverse transcription-recombinase
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polymerase amplification
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RUNNING TITLE: Rapid dengue diagnosis by RT-RPA
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Boon-Teong Teoh1, Sing-Sin Sam1, Kim-Kee Tan1, Mohammed Bashar Danlami1,
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Meng-Hooi Shu1, Jefree Johari1, Poh-Sim Hooi1, David Brooks2, Olaf Piepenburg2,
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Oliver Nentwich2, Annelies Wilder-Smith3,4, Leticia Franco5, Antonio Tenorio5, and
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Sazaly AbuBakar1,*
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1
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of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur,
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Malaysia
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2
15
Kingdom
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3
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Health, Umeå University, Umeå, Sweden
18
4
Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore
19
5
Arbovirus and Imported Viral Diseases, National Centre for Microbiology, Institute
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of Health Carlos III, Madrid, Spain
Tropical Infectious Diseases Research and Education Centre (TIDREC), Department
TwistDx Ltd., Minerva Building, Babraham Research Campus, Cambridge, United Department of Public Health and Clinical Medicine, Epidemiology and Global
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*Corresponding author
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Sazaly AbuBakar
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Email: [email protected]
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WORD COUNT
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Abstract: 239/250 words
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Importance: 129/150 words
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Main text: 3778 words
30
1
31
ABSTRACT
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A method for the rapid diagnosis of early dengue virus (DENV) infection is highly
33
needed. Here, a prototype reverse transcription-recombinase polymerase
34
amplification (RT-RPA) assay was developed. The assay detected DENV RNA in
35
1
TITLE: Early detection of the dengue virus using reverse transcription-recombinase
2
polymerase amplification
3 4
RUNNING TITLE: Rapid dengue diagnosis by RT-RPA
5 6
Boon-Teong Teoh1, Sing-Sin Sam1, Kim-Kee Tan1, Mohammed Bashar Danlami1,
7
Meng-Hooi Shu1, Jefree Johari1, Poh-Sim Hooi1, David Brooks2, Olaf Piepenburg2,
8
Oliver Nentwich2, Annelies Wilder-Smith3,4, Leticia Franco5, Antonio Tenorio5, and
9
Sazaly AbuBakar1,*
10 11
1
12
of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur,
13
Malaysia
14
2
15
Kingdom
16
3
17
Health, Umeå University, Umeå, Sweden
18
4
Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore
19
5
Arbovirus and Imported Viral Diseases, National Centre for Microbiology, Institute
20
of Health Carlos III, Madrid, Spain
Tropical Infectious Diseases Research and Education Centre (TIDREC), Department
TwistDx Ltd., Minerva Building, Babraham Research Campus, Cambridge, United Department of Public Health and Clinical Medicine, Epidemiology and Global
21 22
*Corresponding author
23
Sazaly AbuBakar
24
Email: [email protected]
25 26
WORD COUNT
27
Abstract: 239/250 words
28
Importance: 129/150 words
29
Main text: 3778 words
30
1
31
ABSTRACT
32
A method for the rapid diagnosis of early dengue virus (DENV) infection is highly
33
needed. Here, a prototype reverse transcription-recombinase polymerase
34
amplification (RT-RPA) assay was developed. The assay detected DENV RNA in
35
