HHS Public Access Author manuscript Author Manuscript
Proc SPIE Int Soc Opt Eng. Author manuscript; available in PMC 2017 August 23. Published in final edited form as: Proc SPIE Int Soc Opt Eng. 2014 ; 9037: . doi:10.1117/12.2044443.
eeDAP: An Evaluation Environment for Digital and Analog Pathology Brandon D. Gallasa, Wei-Chung Chenga, Marios A. Gavrielidesa, Adam Ivanskya, Tyler Keaya, Adam Wunderlicha, Jason Hippb, and Stephen M. Hewittb
Author Manuscript
aDivision
of Imaging and Applied Mathematics, OSEL/CDRH/FDA, Silver Spring, MD bLaboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland
Abstract Purpose—The purpose of this work is to present a platform for designing and executing studies that compare pathologists interpreting histopathology of whole slide images (WSI) on a computer display to pathologists interpreting glass slides on an optical microscope.
Author Manuscript
Methods—Here we present eeDAP, an evaluation environment for digital and analog pathology. The key element in eeDAP is the registration of the WSI to the glass slide. Registration is accomplished through computer control of the microscope stage and a camera mounted on the microscope that acquires images of the real time microscope view. Registration allows for the evaluation of the same regions of interest (ROIs) in both domains. This can reduce or eliminate disagreements that arise from pathologists interpreting different areas and focuses the comparison on image quality. Results—We reduced the pathologist interpretation area from an entire glass slide (≈10–30 mm)2 to small ROIs
Proc SPIE Int Soc Opt Eng. Author manuscript; available in PMC 2017 August 23. Published in final edited form as: Proc SPIE Int Soc Opt Eng. 2014 ; 9037: . doi:10.1117/12.2044443.
eeDAP: An Evaluation Environment for Digital and Analog Pathology Brandon D. Gallasa, Wei-Chung Chenga, Marios A. Gavrielidesa, Adam Ivanskya, Tyler Keaya, Adam Wunderlicha, Jason Hippb, and Stephen M. Hewittb
Author Manuscript
aDivision
of Imaging and Applied Mathematics, OSEL/CDRH/FDA, Silver Spring, MD bLaboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland
Abstract Purpose—The purpose of this work is to present a platform for designing and executing studies that compare pathologists interpreting histopathology of whole slide images (WSI) on a computer display to pathologists interpreting glass slides on an optical microscope.
Author Manuscript
Methods—Here we present eeDAP, an evaluation environment for digital and analog pathology. The key element in eeDAP is the registration of the WSI to the glass slide. Registration is accomplished through computer control of the microscope stage and a camera mounted on the microscope that acquires images of the real time microscope view. Registration allows for the evaluation of the same regions of interest (ROIs) in both domains. This can reduce or eliminate disagreements that arise from pathologists interpreting different areas and focuses the comparison on image quality. Results—We reduced the pathologist interpretation area from an entire glass slide (≈10–30 mm)2 to small ROIs
