Int J Clin Exp Med 2014;7(3):540-548 www.ijcem.com /ISSN:1940-5901/IJCEM1401007
Original Article Effect of Cbfa1 on osteogenic differentiation of mesenchymal stem cells under hypoxia condition Zhang-Hua Li1*, Wen Liao2*, Qiang Zhao3, Tang Huan1, Pan Feng1, Xia Wei3, Yang Yi4, Ning-Sheng Shao3 Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China; 2Hubei University of Chinese Medicine, Wuhan 430065, China; 3Laboratory of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China; 4Wuhan Sports University, Wuhan 430079, China. *Equal contributors. 1
Received January 3, 2014; Accepted February 10, 2014; Epub March 15, 2014; Published March 30, 2014 Abstract: Objective: To observe the effect of Cbfa1 on biological characteristics of marrow mesenchymal stem cells under hypoxia. Methods: The second passage of the MSCs were transfected with Cbfa1 and then cultured in 20% O2 and 3% O2 condition individually. The biological features of the cultured MSCs were assessed by the Real-time PCR. Results: After transfected with Cbfa1, the morphology of MSCs was no significant difference between two oxygen concentrations; The RT-PCR examination revealed that the expression of Cbfa1, BMP, OPN and VEGF in MSCs was higher than those before Cbfa1 transfection (P