Int J Clin Exp Med 2014;7(3):540-548 www.ijcem.com /ISSN:1940-5901/IJCEM1401007

Original Article Effect of Cbfa1 on osteogenic differentiation of mesenchymal stem cells under hypoxia condition Zhang-Hua Li1*, Wen Liao2*, Qiang Zhao3, Tang Huan1, Pan Feng1, Xia Wei3, Yang Yi4, Ning-Sheng Shao3 Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China; 2Hubei University of Chinese Medicine, Wuhan 430065, China; 3Laboratory of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China; 4Wuhan Sports University, Wuhan 430079, China. *Equal contributors. 1

Received January 3, 2014; Accepted February 10, 2014; Epub March 15, 2014; Published March 30, 2014 Abstract: Objective: To observe the effect of Cbfa1 on biological characteristics of marrow mesenchymal stem cells under hypoxia. Methods: The second passage of the MSCs were transfected with Cbfa1 and then cultured in 20% O2 and 3% O2 condition individually. The biological features of the cultured MSCs were assessed by the Real-time PCR. Results: After transfected with Cbfa1, the morphology of MSCs was no significant difference between two oxygen concentrations; The RT-PCR examination revealed that the expression of Cbfa1, BMP, OPN and VEGF in MSCs was higher than those before Cbfa1 transfection (P

Effect of Cbfa1 on osteogenic differentiation of mesenchymal stem cells under hypoxia condition.

To observe the effect of Cbfa1 on biological characteristics of marrow mesenchymal stem cells under hypoxia...
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