Pltotorhemslrv and Photobiology, 1978, Vol. 27, pp. 363-365.
Pergamon Press. Printed in Great Britain
RESEARCH NOTE
EFFECT OF COLD STORAGE ON INACTIVATION OF E S C H E R I C H I A COLI BY 313 nm IRRADIATION C. NEWMAN* Argonne National Laboratory, Division of Biological and Medical Research, Argonne, IL 60439, U.S.A.
(Received 15 July 1977; accepted 16 September 1977) Abstract-Data are presented showing that the inactivation response of exponential phase Escherichia coli to 313nm irradiation is affected by previous storage of cells at 3°C in M9 buffer. A similar effect of cold storage on the far-UV inactivation response was not observed. Cold storage alone causes exponential loss in cell viability. After 0.95 days, cell viability is only about 15% of that observed immediately after exposure of cells to cold incubation. However, irradiation at 313 nm causes reactivation of these cold-inactivated bacteria.
INTRODUCTION
filter blocked short wavelength (
Pergamon Press. Printed in Great Britain
RESEARCH NOTE
EFFECT OF COLD STORAGE ON INACTIVATION OF E S C H E R I C H I A COLI BY 313 nm IRRADIATION C. NEWMAN* Argonne National Laboratory, Division of Biological and Medical Research, Argonne, IL 60439, U.S.A.
(Received 15 July 1977; accepted 16 September 1977) Abstract-Data are presented showing that the inactivation response of exponential phase Escherichia coli to 313nm irradiation is affected by previous storage of cells at 3°C in M9 buffer. A similar effect of cold storage on the far-UV inactivation response was not observed. Cold storage alone causes exponential loss in cell viability. After 0.95 days, cell viability is only about 15% of that observed immediately after exposure of cells to cold incubation. However, irradiation at 313 nm causes reactivation of these cold-inactivated bacteria.
INTRODUCTION
filter blocked short wavelength (
