Accepted Manuscript Title: Effect of High Fluoride and High Fat on Serum Lipid Levels and Oxidative Stress in Rabbits Author: Liyan Sun Yanhui Gao Wei Zhang Hui Liu Dianjun Sun PII: DOI: Reference:
S1382-6689(14)00246-4 http://dx.doi.org/doi:10.1016/j.etap.2014.10.010 ENVTOX 2105
To appear in:
Environmental Toxicology and Pharmacology
Received date: Revised date: Accepted date:
7-7-2014 9-10-2014 13-10-2014
Please cite this article as: Sun, L., Gao, Y., Zhang, W., Liu, H., Sun, D.,Effect of High Fluoride and High Fat on Serum Lipid Levels and Oxidative Stress in Rabbits, Environmental Toxicology and Pharmacology (2014), http://dx.doi.org/10.1016/j.etap.2014.10.010 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Highlights： 1. High fluoride and high fat increased serum TC and LDL-C levels both individually and synergistically and this would aggravate hypercholesterolemia in experimental animals.
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2. High fat significantly elevated LPO and MDA levels and lowered T-AOC levels in serum of rabbits.
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3. High fluoride significantly increased LPO and MDA levels in serum of rabbits.
1
Page 1 of 21
Title page Title: Effect of High Fluoride and High Fat on Serum Lipid Levels and Oxidative
Authors:
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Liyan Suna, Yanhui Gaoa, Wei Zhanga, Hui Liu, Dianjun Sun*
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Stress in Rabbits
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Affiliations:
Center for Endemic Disease Control, Chinese Center for Disease Control and
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Prevention, Harbin Medical University; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504),
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Harbin 150081, China. *Corresponding author:
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Dianjun Sun, Pro, Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, 157 Baojian Road,
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Harbin 150081, China. Tel: +86 451 86612695; fax: +86 451 86657674; E-mail: [email protected] (D. Sun). a
These authors contributed equally to this work.
2
Page 2 of 21
Abstract: The purpose of this study was to explore the effects of high fluoride and high fat on triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), total antioxidant
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capacity (T-AOC), lipid peroxide (LPO) and malondialdehyde (MDA) in rabbits. A factorial experimental design was used, with two factors (fluoride and fat) and three
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levels. Seventy-two male rabbits were randomly assigned into nine groups according
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to initial weight and serum lipid levels. The rabbits were fed with basic feed, moderate fat feed or high fat feed and drank tap water, fluoridated water at levels of
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50 and 100 mg fluorion/L freely. Biological materials were collected after 5 months, and serum lipid, T-AOC, LPO, and MDA levels were then measured. Using these data,
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the separate and interactive effects of high fluoride and high fat were analyzed. High fluoride and high fat both increased serum levels of TC, HDL-C and LDL-C
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significantly (P0.05) (Figure 3).
us
higher than those in group A. Unlike cholesterol, there was no significant difference
an
Considering weight as the covariate, the results of covariance analysis showed that high fluoride significantly affected TC, HDL-C and LDL-C levels (P0.05). Unlike high fluoride, high fat notly affected all four serum lipid levels (P
S1382-6689(14)00246-4 http://dx.doi.org/doi:10.1016/j.etap.2014.10.010 ENVTOX 2105
To appear in:
Environmental Toxicology and Pharmacology
Received date: Revised date: Accepted date:
7-7-2014 9-10-2014 13-10-2014
Please cite this article as: Sun, L., Gao, Y., Zhang, W., Liu, H., Sun, D.,Effect of High Fluoride and High Fat on Serum Lipid Levels and Oxidative Stress in Rabbits, Environmental Toxicology and Pharmacology (2014), http://dx.doi.org/10.1016/j.etap.2014.10.010 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Highlights： 1. High fluoride and high fat increased serum TC and LDL-C levels both individually and synergistically and this would aggravate hypercholesterolemia in experimental animals.
ip t
2. High fat significantly elevated LPO and MDA levels and lowered T-AOC levels in serum of rabbits.
Ac ce p
te
d
M
an
us
cr
3. High fluoride significantly increased LPO and MDA levels in serum of rabbits.
1
Page 1 of 21
Title page Title: Effect of High Fluoride and High Fat on Serum Lipid Levels and Oxidative
Authors:
cr
Liyan Suna, Yanhui Gaoa, Wei Zhanga, Hui Liu, Dianjun Sun*
ip t
Stress in Rabbits
us
Affiliations:
Center for Endemic Disease Control, Chinese Center for Disease Control and
an
Prevention, Harbin Medical University; Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504),
M
Harbin 150081, China. *Corresponding author:
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d
Dianjun Sun, Pro, Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, 157 Baojian Road,
Ac ce p
Harbin 150081, China. Tel: +86 451 86612695; fax: +86 451 86657674; E-mail: [email protected] (D. Sun). a
These authors contributed equally to this work.
2
Page 2 of 21
Abstract: The purpose of this study was to explore the effects of high fluoride and high fat on triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), total antioxidant
ip t
capacity (T-AOC), lipid peroxide (LPO) and malondialdehyde (MDA) in rabbits. A factorial experimental design was used, with two factors (fluoride and fat) and three
cr
levels. Seventy-two male rabbits were randomly assigned into nine groups according
us
to initial weight and serum lipid levels. The rabbits were fed with basic feed, moderate fat feed or high fat feed and drank tap water, fluoridated water at levels of
an
50 and 100 mg fluorion/L freely. Biological materials were collected after 5 months, and serum lipid, T-AOC, LPO, and MDA levels were then measured. Using these data,
M
the separate and interactive effects of high fluoride and high fat were analyzed. High fluoride and high fat both increased serum levels of TC, HDL-C and LDL-C
d
significantly (P0.05) (Figure 3).
us
higher than those in group A. Unlike cholesterol, there was no significant difference
an
Considering weight as the covariate, the results of covariance analysis showed that high fluoride significantly affected TC, HDL-C and LDL-C levels (P0.05). Unlike high fluoride, high fat notly affected all four serum lipid levels (P
