83
Short Communications Horm. Metab. Res. 11 (1979) 83-84
Effect of Osteotropic Hormones on Cartilage Metabolism S. Havelka, A. Babicky, J. Musilova, D. Rohozkova and B. Tesarek Research Institute of Rheumatology, and Isotope Laboratory, Biological Inst., es. Academy of Sciences, Prague, Cechoslavakia
200
The influences of calcitonin, vitamin 03 and parathormone on parameters of cartilage metabolism were studied in rabbits.
The results are shown in Table land Figure 1. The two signi· ficant differences ~ainst contro1s appeared to be: increased incorporation of Sinto jOint eartilage after PTH; and depressed extraetibility, after proteoglycan elimination, of joint cartilage collagen by pronase after vitamin 0 3 , literary data on effeet of osteotropic hormones on other cartilage than epiphyseal are sean!. Thickening of rat joint cartilage after protraeted CT was reported by Tarsoly and Bucher (1973). Composition of rat costal eartilage d id not change after 3 wecks of PTH and/or CT treatment (Denis and Franchimont 1971). Our results suggesting an anabolie influence of PTH on joint cartilage eould somehow be conn·
150
..
~c; 0
... 0
100
0
~
50
o
J X
J X A
CT 2U/kQ
CT30U/kQ
(n=IO)
(n = 10)
J XA
J X A
*
032400U/kQ PTH 200U/kQ (n = 10)
(n = 6)
J - joint cartilaQe X - xiphoid cartilaQe
A - auricular cartllaQe
*-
0·05 >p >0·01 (evaluated by t test)
Activity of 3S S in rabbit poolcd cartilage specimens after a single dose of calcitonin, vitamin 0 3 , and parathormone in vivo (expressed as % of activity of relevant control groups.)
ected with known positive effects of PTH or its fragments on bone and cartilage formation (Gaillard, Herrmann·Erlee and Hekkelman 1976; Reeve, Hesp, Williams, Hulme, Klener· man, Zanelli, Darby, Tregear and Parsons 1976; Walker 1973).
Tab. I Acute in vivo effects of calcitonin and vitamin 0 3 on protein and uronie acid content and on collagen extraetibility by pronase Rabbit cartilage (pooled specimens)
(n = 10)
(n = 10)
13,5 mg/g wet tissue
11, I mg/g w.!.
10,4 mg/g w.!.
10,2 mg/g W.t
uronic acids
6,4 mg/g wet tissue
4,4 mg/g W.t.
4,4 mg/g w.!.
4,4 mg/g w.!.
collagen extraeted by pronase
15,4 mg/g sampie
15,9 mg/g sampie
3,7 mg/g sampIe
21,6 mg/g sampie
protein
Received: 31 Jan. 1978
0018-5043/79
0132-0083
controls
controls
Vit.0 3 2400 u./kg (n = 10)
CT 30 u./kg (n = 10)
Accepted: 7 Nov. 1978 S 05.00
© 1979
Georg Thieme Publishers
Downloaded by: Universite Laval. Copyrighted material.
In the first experiment 10 male chinchilla rabbits, about 2,5 kg weight, were given a single i.m. injection of synthetic salmon calcitonin (CT) (Sandoz), 2 u. per kg body weight, followed immediately by intraperitoneal administration of 0.5 ml of S3Ssolution in saline with more than 100 /lCi S3S per mt. The animals were saerificed after 24 hrs with i.v, Pentothal. Knee joint eartilage and xiphoid cartilage were gained. The cartilage was then dissolved in a small amount of concentrated HC I 0 4 and after the addition of H2 O2 incinera· ted on a sand bath. The residue was dissolved in 0,5 ml N HCl and 5 ml of Bray's scintillation solution was added. Standard solution and blank were prepared in a similar way. The measured values were expressed as per mille of the given dose per I gof wet tissue, as mean values of groups investigated. The experiment was repeated with a dosage of 30 u.CT per kg weight, including an assessment of auricular cartilage. Ouring a third experiment 10 rabbits were given i.m. vitamin 03 (Vi-03 Wand er) 2400 u.per kg weight, the other condi· tions being the same as above. Again, all three types of eartilage were examined. In a fourth experiment 6 rabbits received bovine parathormone rPTH) (Para-thor-mone Eli lilly) 200 u.per kg weight i.m. Pooled cartilage sampies from the 2nd and 3rd experiments were studies also for protein and glycosaminoglycan content and extractibility of collagen by pronase.
84
Congress-Announcements
Indication of a protective action of vitamin D3 on joint cartilage collagen reminds of its stabilizing effect on collagen of young bones (Mechanic 1976). The present results and our previous unpublished data on enzyme activities in joint cartilage after CT and 1,25-dihydroxycholecalciferol administration (Cerny, Kl{mova, Havel· ka, Roho~kova and Tesllrek 1975) are suggestive 01' a rapid accessibility of joint cartilage by parenteral route.
References Denis, F., P. Franchimont: Europ. J. c1in. biol. Res. 16: 6570 (1971). - Gaillard, P.J., M.P.M. Herrmann-Erlee, J. W. Hekkelman: Calc. Tiss. Res. Suppl. To Vol. 21: 70 74 (1976). - Mechanic, G.L.: Ca\cif. Tiss. Res. Suppl. to Vol. 21: 177184 (1976). - Reeve, J., R. Hesp, D. Williams, P. Hulme, L. Klenerman, J.M. Zanelli, A.J. Darby, G. W. Tregear, J.A. Par· sons: Lancet 1: 1035 -1038 (1976), - Tarsoly, E., O. Bucher: Acta chir. Acad. Sei. hung. 14: 231 237 (1973). - Walker, D.G.: Clin. Orthop. 97: 158-174 (1973). Requcsts for reprints should be addressp.d. to: Dr. S. Havelka. Res. Inst. of Rheumatol. 12850 Prague (CSSR)
April 10-12, 1979
Brussels (Belgium): European Workshop on the Biochemistry and Biophysics of the Pancreatic B-Cell I nforma tion: Drs. A. Herchuelz and A. Sener Laboratory of Experimental Medicine, 115 boulevard de Waterloo, B-I 000 Brussels, Belgium
May 29-31, 1980
L 'Aquila (Italy): International Symposium on Oligozoospermia Information: G. Frajese, Clinica Medica V- Policlinico Umberto I 1-00100 - Roma, Italia, Tel.: 06/490465 - 490530
July 22-27 1980
Brussels (Belgium): 4tH International Cyclic Nucleotide Conference Information: Dr. J.E. Dumont, I.R.I.B.H.N. School ofMedicine, 2, rue Evers B-IOOO Brussels, Belgium
-
Downloaded by: Universite Laval. Copyrighted material.
CONGRESS-ANNOUNCEMENTS
Short Communications Horm. Metab. Res. 11 (1979) 83-84
Effect of Osteotropic Hormones on Cartilage Metabolism S. Havelka, A. Babicky, J. Musilova, D. Rohozkova and B. Tesarek Research Institute of Rheumatology, and Isotope Laboratory, Biological Inst., es. Academy of Sciences, Prague, Cechoslavakia
200
The influences of calcitonin, vitamin 03 and parathormone on parameters of cartilage metabolism were studied in rabbits.
The results are shown in Table land Figure 1. The two signi· ficant differences ~ainst contro1s appeared to be: increased incorporation of Sinto jOint eartilage after PTH; and depressed extraetibility, after proteoglycan elimination, of joint cartilage collagen by pronase after vitamin 0 3 , literary data on effeet of osteotropic hormones on other cartilage than epiphyseal are sean!. Thickening of rat joint cartilage after protraeted CT was reported by Tarsoly and Bucher (1973). Composition of rat costal eartilage d id not change after 3 wecks of PTH and/or CT treatment (Denis and Franchimont 1971). Our results suggesting an anabolie influence of PTH on joint cartilage eould somehow be conn·
150
..
~c; 0
... 0
100
0
~
50
o
J X
J X A
CT 2U/kQ
CT30U/kQ
(n=IO)
(n = 10)
J XA
J X A
*
032400U/kQ PTH 200U/kQ (n = 10)
(n = 6)
J - joint cartilaQe X - xiphoid cartilaQe
A - auricular cartllaQe
*-
0·05 >p >0·01 (evaluated by t test)
Activity of 3S S in rabbit poolcd cartilage specimens after a single dose of calcitonin, vitamin 0 3 , and parathormone in vivo (expressed as % of activity of relevant control groups.)
ected with known positive effects of PTH or its fragments on bone and cartilage formation (Gaillard, Herrmann·Erlee and Hekkelman 1976; Reeve, Hesp, Williams, Hulme, Klener· man, Zanelli, Darby, Tregear and Parsons 1976; Walker 1973).
Tab. I Acute in vivo effects of calcitonin and vitamin 0 3 on protein and uronie acid content and on collagen extraetibility by pronase Rabbit cartilage (pooled specimens)
(n = 10)
(n = 10)
13,5 mg/g wet tissue
11, I mg/g w.!.
10,4 mg/g w.!.
10,2 mg/g W.t
uronic acids
6,4 mg/g wet tissue
4,4 mg/g W.t.
4,4 mg/g w.!.
4,4 mg/g w.!.
collagen extraeted by pronase
15,4 mg/g sampie
15,9 mg/g sampie
3,7 mg/g sampIe
21,6 mg/g sampie
protein
Received: 31 Jan. 1978
0018-5043/79
0132-0083
controls
controls
Vit.0 3 2400 u./kg (n = 10)
CT 30 u./kg (n = 10)
Accepted: 7 Nov. 1978 S 05.00
© 1979
Georg Thieme Publishers
Downloaded by: Universite Laval. Copyrighted material.
In the first experiment 10 male chinchilla rabbits, about 2,5 kg weight, were given a single i.m. injection of synthetic salmon calcitonin (CT) (Sandoz), 2 u. per kg body weight, followed immediately by intraperitoneal administration of 0.5 ml of S3Ssolution in saline with more than 100 /lCi S3S per mt. The animals were saerificed after 24 hrs with i.v, Pentothal. Knee joint eartilage and xiphoid cartilage were gained. The cartilage was then dissolved in a small amount of concentrated HC I 0 4 and after the addition of H2 O2 incinera· ted on a sand bath. The residue was dissolved in 0,5 ml N HCl and 5 ml of Bray's scintillation solution was added. Standard solution and blank were prepared in a similar way. The measured values were expressed as per mille of the given dose per I gof wet tissue, as mean values of groups investigated. The experiment was repeated with a dosage of 30 u.CT per kg weight, including an assessment of auricular cartilage. Ouring a third experiment 10 rabbits were given i.m. vitamin 03 (Vi-03 Wand er) 2400 u.per kg weight, the other condi· tions being the same as above. Again, all three types of eartilage were examined. In a fourth experiment 6 rabbits received bovine parathormone rPTH) (Para-thor-mone Eli lilly) 200 u.per kg weight i.m. Pooled cartilage sampies from the 2nd and 3rd experiments were studies also for protein and glycosaminoglycan content and extractibility of collagen by pronase.
84
Congress-Announcements
Indication of a protective action of vitamin D3 on joint cartilage collagen reminds of its stabilizing effect on collagen of young bones (Mechanic 1976). The present results and our previous unpublished data on enzyme activities in joint cartilage after CT and 1,25-dihydroxycholecalciferol administration (Cerny, Kl{mova, Havel· ka, Roho~kova and Tesllrek 1975) are suggestive 01' a rapid accessibility of joint cartilage by parenteral route.
References Denis, F., P. Franchimont: Europ. J. c1in. biol. Res. 16: 6570 (1971). - Gaillard, P.J., M.P.M. Herrmann-Erlee, J. W. Hekkelman: Calc. Tiss. Res. Suppl. To Vol. 21: 70 74 (1976). - Mechanic, G.L.: Ca\cif. Tiss. Res. Suppl. to Vol. 21: 177184 (1976). - Reeve, J., R. Hesp, D. Williams, P. Hulme, L. Klenerman, J.M. Zanelli, A.J. Darby, G. W. Tregear, J.A. Par· sons: Lancet 1: 1035 -1038 (1976), - Tarsoly, E., O. Bucher: Acta chir. Acad. Sei. hung. 14: 231 237 (1973). - Walker, D.G.: Clin. Orthop. 97: 158-174 (1973). Requcsts for reprints should be addressp.d. to: Dr. S. Havelka. Res. Inst. of Rheumatol. 12850 Prague (CSSR)
April 10-12, 1979
Brussels (Belgium): European Workshop on the Biochemistry and Biophysics of the Pancreatic B-Cell I nforma tion: Drs. A. Herchuelz and A. Sener Laboratory of Experimental Medicine, 115 boulevard de Waterloo, B-I 000 Brussels, Belgium
May 29-31, 1980
L 'Aquila (Italy): International Symposium on Oligozoospermia Information: G. Frajese, Clinica Medica V- Policlinico Umberto I 1-00100 - Roma, Italia, Tel.: 06/490465 - 490530
July 22-27 1980
Brussels (Belgium): 4tH International Cyclic Nucleotide Conference Information: Dr. J.E. Dumont, I.R.I.B.H.N. School ofMedicine, 2, rue Evers B-IOOO Brussels, Belgium
-
Downloaded by: Universite Laval. Copyrighted material.
CONGRESS-ANNOUNCEMENTS
