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Effect of Pentagastrin, Histalog, Glucagon, Secretin, and Perchlorate on the Gastric Handling of 99mTc Pertechnetate in Mice 1
Nuclear Medicine
Jayant Khettery, M.D., Eric Elfmann, M.D.,2 Richard J. Grand, M.D.,3 and Salvador Treves, M.D. Pentagastrin, histalog, glucagon, secretin, and perchlorate were intravenously injected into mice in order to investigate the hormonal regulation of 99mrc pertechnetate uptake by gastric mucosa. Pentagastrin significantly increased uptake; the effect of histalog was weaker. Secretin did not alter the uptake. Glucagon produced some diminution, and perchlorate significantly inhibited gastric uptake. Hormones may playa role in improving the sensitivity and specificity of diagnostic tests for Meckel's diverticulum. INDEX TERMS: ·Glucagon. (Meckel's Diverticulum, 74.1493) • Pentagastrin • Perchlorate • Secretin • Stomach. mucosa • Stomach, radionuclide studies
Radiology 120:629-631, September 1976
OLLOWING intravenous injection, technetium99m pertechnetate is rapidly taken up by the stomach, salivary glands, mucosal lining of the nasal sinus, thyroid gland, and choroid plexus; it is excreted via the kidneys (1, 2). Harper et al. demonstrated that about 25 % of the injected dose of 99mTc pertechnetate localizes in the stomach (1). Using microautoradiography, Meier-Ruge and Fridrich (3) demonstrated that 99mTc as sodium pertechnetate is selectively taken up by the parietal cells of the gastric mucosa, whereas 131 1 as sodium iodine was absorbed and secreted by the gastric chief and mucosal cells. It was therefore suggested that the selective accumulation of 99mTC pertechnetate in the parietal cells may represent a selective metabolic pathway for its secretion (3). In an effort to elucidate the role of hormones in the gastric uptake and secretion of 99mTc, we studied the effects of histalog, pentagastrin, secretin, glucagon, and perchlorate on this process.
to 60 min. and later; the stomach was exposed and tied at the esophageal and duodenal ends, removed, weighed, and counted for radioactivity in a well counter (In-V-tron, Nuclear Systems, Texas). The stomach was subsequently opened, washed, weighed, and counted for radioactivity. The fractional per cent uptake (per cent of injected dose of radioactivity in the stomach) for the whole stomach, as well as for the washed empty stomach, was computed. Radioactivity per gram of stomach, calculated as per cent of injected dose, was also determined. A separate set of control values was obtained with each experiment.
F
RESULTS
Thirty minutes following injection, pentagastrin produced a 40% increase in the fractional uptake of 99mTc pertechnetate by the whole stomach compared to control (Fig. 1). The uptake by the washed stomach was also higher, as was uptake per gram of tissue in the whole and washed stomach. Because of the borderline statistical significance of the increase in uptake at 30 min. (p
Effect of Pentagastrin, Histalog, Glucagon, Secretin, and Perchlorate on the Gastric Handling of 99mTc Pertechnetate in Mice 1
Nuclear Medicine
Jayant Khettery, M.D., Eric Elfmann, M.D.,2 Richard J. Grand, M.D.,3 and Salvador Treves, M.D. Pentagastrin, histalog, glucagon, secretin, and perchlorate were intravenously injected into mice in order to investigate the hormonal regulation of 99mrc pertechnetate uptake by gastric mucosa. Pentagastrin significantly increased uptake; the effect of histalog was weaker. Secretin did not alter the uptake. Glucagon produced some diminution, and perchlorate significantly inhibited gastric uptake. Hormones may playa role in improving the sensitivity and specificity of diagnostic tests for Meckel's diverticulum. INDEX TERMS: ·Glucagon. (Meckel's Diverticulum, 74.1493) • Pentagastrin • Perchlorate • Secretin • Stomach. mucosa • Stomach, radionuclide studies
Radiology 120:629-631, September 1976
OLLOWING intravenous injection, technetium99m pertechnetate is rapidly taken up by the stomach, salivary glands, mucosal lining of the nasal sinus, thyroid gland, and choroid plexus; it is excreted via the kidneys (1, 2). Harper et al. demonstrated that about 25 % of the injected dose of 99mTc pertechnetate localizes in the stomach (1). Using microautoradiography, Meier-Ruge and Fridrich (3) demonstrated that 99mTc as sodium pertechnetate is selectively taken up by the parietal cells of the gastric mucosa, whereas 131 1 as sodium iodine was absorbed and secreted by the gastric chief and mucosal cells. It was therefore suggested that the selective accumulation of 99mTC pertechnetate in the parietal cells may represent a selective metabolic pathway for its secretion (3). In an effort to elucidate the role of hormones in the gastric uptake and secretion of 99mTc, we studied the effects of histalog, pentagastrin, secretin, glucagon, and perchlorate on this process.
to 60 min. and later; the stomach was exposed and tied at the esophageal and duodenal ends, removed, weighed, and counted for radioactivity in a well counter (In-V-tron, Nuclear Systems, Texas). The stomach was subsequently opened, washed, weighed, and counted for radioactivity. The fractional per cent uptake (per cent of injected dose of radioactivity in the stomach) for the whole stomach, as well as for the washed empty stomach, was computed. Radioactivity per gram of stomach, calculated as per cent of injected dose, was also determined. A separate set of control values was obtained with each experiment.
F
RESULTS
Thirty minutes following injection, pentagastrin produced a 40% increase in the fractional uptake of 99mTc pertechnetate by the whole stomach compared to control (Fig. 1). The uptake by the washed stomach was also higher, as was uptake per gram of tissue in the whole and washed stomach. Because of the borderline statistical significance of the increase in uptake at 30 min. (p
