Korean J Physiol Pharmacol 2017;21(2):153-160 https://doi.org/10.4196/kjpp.2017.21.2.153
Original Article
Effect of subcutaneous treatment with human umbilical cord blood-derived multipotent stem cells on peripheral neuropathic pain in rats Min Ju Lee1,#, Tae Gyoon Yoon2,#, Moonkyu Kang1, Hyun Jeong Kim1,*, and Kyung Sun Kang3,* 1
Department of Dental Anesthesiology and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, 2Department of Anesthesiology and Pain Medicine, Konkuk University Medical Center, Research Institute of Medical Science, Konkuk University School of Medicine, Seoul 05030, 3Adult Stem Cell Research Center, Laboratory of Stem Cell and Tumor Biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
ARTICLE INFO Received July 1, 2016 Revised August 23, 2016 Accepted November 29, 2016
*Correspondence Hyun Jeong Kim E-mail: [email protected]
Kyung Sun Kang E-mail: [email protected]
Key Words Cord blood stem cell transplantation Neuropathic pain Rat subcutaneous injection Tissue inhibitor of metalloproteinase 2 #These authors contributed equally to this work.
ABSTRACT In this study, we aim to determine the in vivo effect of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs) on neuropathic pain, using three, principal peripheral neuropathic pain models. Four weeks after hUCBMSC transplantation, we observed significant antinociceptive effect in hUCB-MSC– transplanted rats compared to that in the vehicle-treated control. Spinal cord cells positive for c-fos, CGRP, p-ERK, p-p 38, MMP-9 and MMP 2 were significantly decreased in only CCI model of hUCB-MSCs-grafted rats, while spinal cord cells positive for CGRP, p-ERK and MMP-2 significantly decreased in SNL model of hUCBMSCs-grafted rats and spinal cord cells positive for CGRP and MMP-2 significantly decreased in SNI model of hUCB-MSCs-grafted rats, compared to the control 4 weeks or 8weeks after transplantation (p
Original Article
Effect of subcutaneous treatment with human umbilical cord blood-derived multipotent stem cells on peripheral neuropathic pain in rats Min Ju Lee1,#, Tae Gyoon Yoon2,#, Moonkyu Kang1, Hyun Jeong Kim1,*, and Kyung Sun Kang3,* 1
Department of Dental Anesthesiology and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, 2Department of Anesthesiology and Pain Medicine, Konkuk University Medical Center, Research Institute of Medical Science, Konkuk University School of Medicine, Seoul 05030, 3Adult Stem Cell Research Center, Laboratory of Stem Cell and Tumor Biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
ARTICLE INFO Received July 1, 2016 Revised August 23, 2016 Accepted November 29, 2016
*Correspondence Hyun Jeong Kim E-mail: [email protected]
Kyung Sun Kang E-mail: [email protected]
Key Words Cord blood stem cell transplantation Neuropathic pain Rat subcutaneous injection Tissue inhibitor of metalloproteinase 2 #These authors contributed equally to this work.
ABSTRACT In this study, we aim to determine the in vivo effect of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs) on neuropathic pain, using three, principal peripheral neuropathic pain models. Four weeks after hUCBMSC transplantation, we observed significant antinociceptive effect in hUCB-MSC– transplanted rats compared to that in the vehicle-treated control. Spinal cord cells positive for c-fos, CGRP, p-ERK, p-p 38, MMP-9 and MMP 2 were significantly decreased in only CCI model of hUCB-MSCs-grafted rats, while spinal cord cells positive for CGRP, p-ERK and MMP-2 significantly decreased in SNL model of hUCBMSCs-grafted rats and spinal cord cells positive for CGRP and MMP-2 significantly decreased in SNI model of hUCB-MSCs-grafted rats, compared to the control 4 weeks or 8weeks after transplantation (p
