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Original paper
Effects and mechanisms of auricular electroacupuncture on visceral pain induced by colorectal distension in conscious rats Han Li,1 Shasha Hu,1 Jianbin Zhang,2 Jingzhu Zhou,1 Hongxing Ran,1 Yichun Tang,2 Jiande Chen,3 Yinping Wang1
1
Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China 2 Department of Acuology, The Second Clinical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China 3 Division of Gastroenterology, University of Texas Medical Branch, Galveston, Texas, USA Correspondence to Professor Yinping Wang, Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Nanjing Medical University, No 300 Guangzhou Road, Gulou district, Nanjing, Jiangsu Province, China; [email protected] HL, SH, JZhang, JZhou are considered as the first coauthors. Received 3 April 2014 Revised 16 July 2014 Accepted 16 August 2014
To cite: Li H, Hu S, Zhang J, et al. Acupunct Med Published Online First: [ please include Day Month Year] doi:10.1136/acupmed-2014010575
ABSTRACT Objective To investigate the effects and mechanisms of action of auricular electroacupuncture (AEA) on visceral pain induced by colorectal distension (CRD). Methods Twenty-nine female Sprague-Dawley rats were randomly divided into four groups: control; untreated CRD; CRD+AEA; and CRD+sham electroacupuncture (SEA). An electromyogram (EMG) was recorded for 120 min in the conscious state. After a 30 min baseline recording, CRD was performed in untreated CRD, AEA and SEA groups and lasted for 90 min. AEA and SEA were started at 30 min and lasted for 30 min. The EMG was recorded and analysed to evaluate the severity of visceral pain, indicated by the magnitude of the vasomotor response (VMR). mRNA expression of the 5-hydroxytryptamine 1a (5-HT1a) receptor was measured separately in the colon and raphe nuclei using real-time fluorescent quantitative PCR. Results No differences were seen in the baseline EMG among the four groups (p>0.05). During prestimulation, VMR magnitude in the CRD, AEA and SEA groups increased compared with that in the control group (p
Original paper
Effects and mechanisms of auricular electroacupuncture on visceral pain induced by colorectal distension in conscious rats Han Li,1 Shasha Hu,1 Jianbin Zhang,2 Jingzhu Zhou,1 Hongxing Ran,1 Yichun Tang,2 Jiande Chen,3 Yinping Wang1
1
Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China 2 Department of Acuology, The Second Clinical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China 3 Division of Gastroenterology, University of Texas Medical Branch, Galveston, Texas, USA Correspondence to Professor Yinping Wang, Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Nanjing Medical University, No 300 Guangzhou Road, Gulou district, Nanjing, Jiangsu Province, China; [email protected] HL, SH, JZhang, JZhou are considered as the first coauthors. Received 3 April 2014 Revised 16 July 2014 Accepted 16 August 2014
To cite: Li H, Hu S, Zhang J, et al. Acupunct Med Published Online First: [ please include Day Month Year] doi:10.1136/acupmed-2014010575
ABSTRACT Objective To investigate the effects and mechanisms of action of auricular electroacupuncture (AEA) on visceral pain induced by colorectal distension (CRD). Methods Twenty-nine female Sprague-Dawley rats were randomly divided into four groups: control; untreated CRD; CRD+AEA; and CRD+sham electroacupuncture (SEA). An electromyogram (EMG) was recorded for 120 min in the conscious state. After a 30 min baseline recording, CRD was performed in untreated CRD, AEA and SEA groups and lasted for 90 min. AEA and SEA were started at 30 min and lasted for 30 min. The EMG was recorded and analysed to evaluate the severity of visceral pain, indicated by the magnitude of the vasomotor response (VMR). mRNA expression of the 5-hydroxytryptamine 1a (5-HT1a) receptor was measured separately in the colon and raphe nuclei using real-time fluorescent quantitative PCR. Results No differences were seen in the baseline EMG among the four groups (p>0.05). During prestimulation, VMR magnitude in the CRD, AEA and SEA groups increased compared with that in the control group (p
