Accepted Manuscript Effects of exposure to multiple heavy metals on biochemical and histopathological alterations in common carp, Cyprinus carpio L. Sivakumar Rajeshkumar, Yang Liu, Junguo Ma, Hong Ying Duan, Xiaoyu Li PII:
S1050-4648(17)30474-6
DOI:
10.1016/j.fsi.2017.08.013
Reference:
YFSIM 4755
To appear in:
Fish and Shellfish Immunology
Received Date: 18 May 2017 Revised Date:
26 July 2017
Accepted Date: 11 August 2017
Please cite this article as: Rajeshkumar S, Liu Y, Ma J, Duan HY, Li X, Effects of exposure to multiple heavy metals on biochemical and histopathological alterations in common carp, Cyprinus carpio L., Fish and Shellfish Immunology (2017), doi: 10.1016/j.fsi.2017.08.013. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT
1
Effects of exposure to multiple heavy metals on biochemical and histopathological alterations in
2
common carp, Cyprinus carpio L.
3
Sivakumar Rajeshkumar, Yang Liu, Junguo Ma, Hong Ying Duan, Xiaoyu Li*
RI PT
4 5 6
College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China
SC
7 8
M AN U
9 10 11 12
16 17 18 19 20 21
EP
15
AC C
14
TE D
13
22 23 24
*Corresponding author. Tel.: +86-373-3329390; Fax: +86-373-3329390 E-mail address: [email protected] (Xiaoyu Li).
ACCEPTED MANUSCRIPT
25
Abstract
26
Heavy metals are frequently encountered as mixtures of essential and non-essential
28
elements. Therefore, evaluation of their toxic effects individually does not offer a realistic
29
estimate of their impact on biological processes. We studied effects of exposure to mixtures of
30
essential and toxic metals (Cr, Cd and Pb) on biochemical, immunotoxicity level and
31
morphological characteristics of the various tissues of a biomarker freshwater fish common carp
32
using environmentally relevant concentrations. Fish were exposed to metal mixture through tank
33
water for 7, 15 and 30 days, under controlled laboratory conditions. Tissue accumulation of the
34
metals was measured using Atomic Absorption Spectrophotometric techniques. Chromium,
35
cadmium and lead accumulation in muscle, gills, liver, kidney and intestine, tissue of common
36
carp exposed to mixture metals for 30 days increased significant compared with control group
37
(p
S1050-4648(17)30474-6
DOI:
10.1016/j.fsi.2017.08.013
Reference:
YFSIM 4755
To appear in:
Fish and Shellfish Immunology
Received Date: 18 May 2017 Revised Date:
26 July 2017
Accepted Date: 11 August 2017
Please cite this article as: Rajeshkumar S, Liu Y, Ma J, Duan HY, Li X, Effects of exposure to multiple heavy metals on biochemical and histopathological alterations in common carp, Cyprinus carpio L., Fish and Shellfish Immunology (2017), doi: 10.1016/j.fsi.2017.08.013. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT
1
Effects of exposure to multiple heavy metals on biochemical and histopathological alterations in
2
common carp, Cyprinus carpio L.
3
Sivakumar Rajeshkumar, Yang Liu, Junguo Ma, Hong Ying Duan, Xiaoyu Li*
RI PT
4 5 6
College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China
SC
7 8
M AN U
9 10 11 12
16 17 18 19 20 21
EP
15
AC C
14
TE D
13
22 23 24
*Corresponding author. Tel.: +86-373-3329390; Fax: +86-373-3329390 E-mail address: [email protected] (Xiaoyu Li).
ACCEPTED MANUSCRIPT
25
Abstract
26
Heavy metals are frequently encountered as mixtures of essential and non-essential
28
elements. Therefore, evaluation of their toxic effects individually does not offer a realistic
29
estimate of their impact on biological processes. We studied effects of exposure to mixtures of
30
essential and toxic metals (Cr, Cd and Pb) on biochemical, immunotoxicity level and
31
morphological characteristics of the various tissues of a biomarker freshwater fish common carp
32
using environmentally relevant concentrations. Fish were exposed to metal mixture through tank
33
water for 7, 15 and 30 days, under controlled laboratory conditions. Tissue accumulation of the
34
metals was measured using Atomic Absorption Spectrophotometric techniques. Chromium,
35
cadmium and lead accumulation in muscle, gills, liver, kidney and intestine, tissue of common
36
carp exposed to mixture metals for 30 days increased significant compared with control group
37
(p
