820
Lu et al. / J Zhejiang Univ-Sci B (Biomed & Biotechnol) 2014 15(9):820-829
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) ISSN 1673-1581 (Print); ISSN 1862-1783 (Online) www.zju.edu.cn/jzus; www.springerlink.com E-mail: [email protected]
Effects of hypoxic exercise training on microRNA expression and lipid metabolism in obese rat livers* Ying-li LU§1, Wen JING§2, Lian-shi FENG†‡1, Li ZHANG1, Jian-fang XU1, Tong-jian YOU3, Jing ZHAO†‡4 (1Biology Center, China Institute of Sport Science, Beijing 100061, China) (2Sport Science Research Center of Shandong Province, Jinan 250102, China) 3
( Department of Exercise and Health Sciences, University of Massachusetts Boston, Boston, MA 02125, USA) (4School of Life Sciences, Shandong University, Jinan 250100, China) †
E-mail: [email protected]; [email protected]
Received Feb. 21, 2014; Revision accepted July 8, 2014; Crosschecked Aug. 15, 2014
Abstract: To investigate the effects of hypoxic exercise training on microRNA (miRNA) expression and the role of miRNA expression in regulating lipid metabolism, 20 dietary-induced obese SD rats were divided into a normoxic sedentary group (N, n=10) and a hypoxic exercise training group (H, n=10). After four weeks, measurements were taken of body weight, body length, fat mass, serum lipid concentration, miRNAs differentially expressed in rat liver, and gene and protein expression levels of peroxisome proliferator activated receptor α (PPARα), fatty acid synthetase (FAS), and carnitine palmitoyl transferase 1A (CPT1A) in rat liver. Body weight, Lee’s index, fat mass, fat/weight ratio, and serum levels of total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) were all significantly lower in the H group than in the N group (P32, the miRNA was considered detectable (Chen et al., 2012). Significance analysis of microarrays was performed to identify the significantly altered miRNA. The miRNA with an absolute fold change (H/C group) >1.5 and P
Lu et al. / J Zhejiang Univ-Sci B (Biomed & Biotechnol) 2014 15(9):820-829
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) ISSN 1673-1581 (Print); ISSN 1862-1783 (Online) www.zju.edu.cn/jzus; www.springerlink.com E-mail: [email protected]
Effects of hypoxic exercise training on microRNA expression and lipid metabolism in obese rat livers* Ying-li LU§1, Wen JING§2, Lian-shi FENG†‡1, Li ZHANG1, Jian-fang XU1, Tong-jian YOU3, Jing ZHAO†‡4 (1Biology Center, China Institute of Sport Science, Beijing 100061, China) (2Sport Science Research Center of Shandong Province, Jinan 250102, China) 3
( Department of Exercise and Health Sciences, University of Massachusetts Boston, Boston, MA 02125, USA) (4School of Life Sciences, Shandong University, Jinan 250100, China) †
E-mail: [email protected]; [email protected]
Received Feb. 21, 2014; Revision accepted July 8, 2014; Crosschecked Aug. 15, 2014
Abstract: To investigate the effects of hypoxic exercise training on microRNA (miRNA) expression and the role of miRNA expression in regulating lipid metabolism, 20 dietary-induced obese SD rats were divided into a normoxic sedentary group (N, n=10) and a hypoxic exercise training group (H, n=10). After four weeks, measurements were taken of body weight, body length, fat mass, serum lipid concentration, miRNAs differentially expressed in rat liver, and gene and protein expression levels of peroxisome proliferator activated receptor α (PPARα), fatty acid synthetase (FAS), and carnitine palmitoyl transferase 1A (CPT1A) in rat liver. Body weight, Lee’s index, fat mass, fat/weight ratio, and serum levels of total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) were all significantly lower in the H group than in the N group (P32, the miRNA was considered detectable (Chen et al., 2012). Significance analysis of microarrays was performed to identify the significantly altered miRNA. The miRNA with an absolute fold change (H/C group) >1.5 and P
