J Anesth DOI 10.1007/s00540-015-2026-8
SHORT COMMUNICATION
Effects of levobupivacaine on isolated rat tracheal smooth muscle Hung‑Chi Chang1,2 · Shin‑Yan Chen1 · Yu‑Feng Huang1 · Feng‑Lin Liu1 · Yih‑Giun Cherng1,3 · Hsing‑Won Wang4,5
Received: 22 September 2014 / Accepted: 30 April 2015 © Japanese Society of Anesthesiologists 2015
Abstract Levobupivacaine has been developed as a safer alternative to bupivacaine because of its reduced systemic toxicity. However, the effect of directly delivering levobupivacaine into tracheal smooth muscle has not been adequately explored. We performed this study to determine the in vitro effects of levobupivacaine on isolated rat tracheal smooth muscle. A portion of rat trachea 5 mm in length was mounted in 30 ml of Krebs solution in a muscle bath at 37 °C. The following effects of levobupivacaine were assessed: (1) the effect on tracheal smooth muscle resting tension (n = 6), (2) the effect on contraction caused by 10−6 M methacholine (n = 6) and (3) the effect on electrically induced tracheal smooth muscle contractions (n = 6). Levobupivacaine caused dose-dependent relaxation in the trachealis muscle precontracted with 10−6 M methacholine. Contraction inhibition was statistically significant when 10−5 and 10−4 M levobupivacaine were applied, compared Y.-G. Cherng and H.-W. Wang contributed equally to this work. * Hsing‑Won Wang [email protected] Yih‑Giun Cherng [email protected] 1
Department of Anesthesiology, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan
2
Graduate Institute of Translational and Interdisciplinary Medicine, National Central University, Taoyuan, Taiwan
3
Department of Anesthesiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
4
Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
5
Department of Otolaryngology, Shuang Ho Hospital, Taipei Medical University, No. 291, Jhongjheng Rd., Jhonghe, Taipei 23561, Taiwan
with the contraction inhibition that occurred in the control groups (p
SHORT COMMUNICATION
Effects of levobupivacaine on isolated rat tracheal smooth muscle Hung‑Chi Chang1,2 · Shin‑Yan Chen1 · Yu‑Feng Huang1 · Feng‑Lin Liu1 · Yih‑Giun Cherng1,3 · Hsing‑Won Wang4,5
Received: 22 September 2014 / Accepted: 30 April 2015 © Japanese Society of Anesthesiologists 2015
Abstract Levobupivacaine has been developed as a safer alternative to bupivacaine because of its reduced systemic toxicity. However, the effect of directly delivering levobupivacaine into tracheal smooth muscle has not been adequately explored. We performed this study to determine the in vitro effects of levobupivacaine on isolated rat tracheal smooth muscle. A portion of rat trachea 5 mm in length was mounted in 30 ml of Krebs solution in a muscle bath at 37 °C. The following effects of levobupivacaine were assessed: (1) the effect on tracheal smooth muscle resting tension (n = 6), (2) the effect on contraction caused by 10−6 M methacholine (n = 6) and (3) the effect on electrically induced tracheal smooth muscle contractions (n = 6). Levobupivacaine caused dose-dependent relaxation in the trachealis muscle precontracted with 10−6 M methacholine. Contraction inhibition was statistically significant when 10−5 and 10−4 M levobupivacaine were applied, compared Y.-G. Cherng and H.-W. Wang contributed equally to this work. * Hsing‑Won Wang [email protected] Yih‑Giun Cherng [email protected] 1
Department of Anesthesiology, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan
2
Graduate Institute of Translational and Interdisciplinary Medicine, National Central University, Taoyuan, Taiwan
3
Department of Anesthesiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
4
Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
5
Department of Otolaryngology, Shuang Ho Hospital, Taipei Medical University, No. 291, Jhongjheng Rd., Jhonghe, Taipei 23561, Taiwan
with the contraction inhibition that occurred in the control groups (p
